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Download Protrans Sequencing Testkits (SBT)
Transcript
Protrans HLA-DPB1 Testkit Tubes Sequencing Primers Exon 2 forward (blue) Exon 2 reverse (red) S1 x x Agarose-Gel Loading Buffer (colourless) Tube DPBE2F DPBE2R LB S1 µl I 1x 360 -20°C 1x 360 -20°C 400 4-8°C 1x = Content of the Sequencing Unit components Sequencing Primers 5’ and 3’-primers Loading Buffer Gel Loading Solution Note Refer to the Primer Mix Specificity Tables (Attachment) delivered with the kits to select the appropriate PCR products and Sequencing Primers. Make sure that the version of the kit and the version of the Primer Mix Specificity Tables are identical Note All reagents supplied with the Protrans HLA Sequencing Kits should be used Amplification Primer Version specific and Sequencing Primer LOT specific Amplification Primer can not be mixed between different Versions as indicated on the Attachment of each Kit Sequencing Primer can not be mixed between different LOT´s as indicated as indicated on the Attachment of each Kit Note In case of damaged tubes or boxes malfunctions cannot be excluded. Those Kits must not be used. Protrans = User Manual SBT Version 2010 07 01 17
