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Matchmaker™ Co-IP Kit User Manual
I. Introduction continued
Bolster your Co-IP results with other Matchmaker™ assays
The protein interactions identified in a yeast two-hybrid screen and by Co-IP
assay may represent biologically significant associations. After confirming the
association between a pair of mammalian proteins in vitro, you can go on to
verify that these same proteins interact in vivo, in their native environments, using
either the Matchmaker Mammalian Two-Hybrid Assay Kit (Cat. No. 630301) or
the Matchmaker Mammalian Assay Kit 2 (Cat. No. 630305) to perform a twohybrid analysis in mammalian cells, or the pCMV-Myc and pCMV-HA Vector Set
(Cat. No. 631604) to perform an in vivo coimmunoprecipitation in mammalian
cells. These products allow you to evaluate the extent to which posttranslational
modifications and cellular conditions occurring in mammalian systems affect the
interaction between a given pair of proteins.
To map the domains and residues required for protein-protein association, we
recommend our Matchmaker BioSensor Kit (Cat. No. 630446). It provides a
simple fluorescence-based assay for detecting two-hybrid interactions. The
assay is performed in our patented 96-well BioSensor Plate, which measures
yeast growth under selective conditions. After subcloning different variants or
fragments of your cDNA clone(s), you can use the BioSensor Plates to quickly
identify those expression products that interact with your primary bait—each kit
provides sufficient materials to perform over 450 two-hybrid assays.
Clontech Laboratories, Inc.
www.clontech.com
Protocol No. PT3323-1
Version No. PR631435