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Table of Contents Contents and Storage ........................................................................................................................................... v Accessory Products.............................................................................................................................................. vi Introduction ................................................................................................................... 1 Product Information ..............................................................................................................................................1 Methods ......................................................................................................................... 2 General Information ..............................................................................................................................................2 Thawing Cells.........................................................................................................................................................4 Subculturing Cells .................................................................................................................................................5 Freezing Cells .........................................................................................................................................................7 Transfecting Cells ..................................................................................................................................................8 Appendix........................................................................................................................ 9 Map of pCMVSPORT6TAg.neo...........................................................................................................................9 Technical Support ................................................................................................................................................10 Purchaser Notification ........................................................................................................................................11 References .............................................................................................................................................................12 iii