Download ScanArray Express Microarray Analysis System User Manual

System Settings
Chapter 7
8.
!
Note: Using the rainbow palette for the simple images provides more visual data by
providing a spread of intensities instead of limited shades of just one color. This allows
you to differentiate more spots than you can with a single color.
9.
7.4
Click the Palette button to display the palette list. Select the desired palette and click
OK.
Click OK to close the Fluorophore dialog box and save the new fluorophore.
Configuring Barcodes
With an optional barcode reader installed, the ScanArray Express can read barcodes on the
microarrays. Using barcodes as unique identifiers on individual substrates provides a
valuable way for tracking samples and workflow, and automating analysis of microarray
experiments. Using barcodes allows a gene sample to be tracked through the entire
microarray process including printing, hybridization, scanning, quantitation, and
visualization.
For systems that are integrated with ArrayInformatics, unique barcodes on microarrays are
required and allow you to retrieve information from and automatically save data to the
ArrayInformatics database.
7.4.1
Supported Barcodes
The ScanArray Express barcode reader supports Code 128, Code 39, and Interleaved 2 of
5 (ITF) symbologies by default. The ScanArray Express barcode reader may be
configured to support additional symbologies with help from technical support. The
maximum number of digits supported by each symbology depends on the minimum
element width (the barcode “pitch”) in the printed barcode. Alphabetic and numeric digits
can be combined, but alphabetic digits take more space than numbers, thus decreasing the
maximum number of allowed digits.
Table 7-1 lists the maximum number of alphanumeric characters for substrate barcodes.
Table 7-1: Substrate Barcodes
Element Width
User Manual
Number of Characters
Inches
Mm
Code 128
Code 39
ITF
.010”
.254 mm
6
2
6
.0075”
.19 mm
12
4
10
.005”
.127 mm
22
8
16
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