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7. A typical Bradford assay result from a commercial plate reader is illustrated in
Figure 6 on the next page.
Figure 6. Print out from a Bradford plate-based protein assay.
C. Using a NanoDropTM to Measure Antibody Concentration
If the antibody sample is free of protein-based carriers (e.g. BSA, gelatin) or
certain interfering preservatives such as thimerosal, then a simple nondestructive scan of the IgG sample on a NanoDropTM spectrophotometer can
be used to accurately determine its concentration.
To estimate antibody concentration on a NanoDropTM spectrophotometer,
proceed as follows:
Catalog # A-9001-006
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