Download Total RNA Isolation

Total RNA Isolation
Table of contents
1
2
Components
4
1.1 Kit contents
4
1.2 Reagents, consumables, and equipment to be supplied by user
5
1.3 About this User Manual
5
Product description
6
2.1 The basic principle
6
2.2 Kit specifications
6
2.3 Handling, preparation, and storage of starting materials
8
2.4 Elution procedures
10
2.5 Stability of isolated RNA
10
3
Storage conditions and preparation of working solutions
11
4
Safety instructions – risk and safety phrases
13
5
Protocols
15
5.1 Total RNA purification from cultured cells, laser captured cells,
or microdissected cryosections with NucleoSpin® RNA XS
15
5.2 Total RNA purification from tissue with NucleoSpin RNA XS
19
®
5.3 Clean-up and concentration of RNA with NucleoSpin RNA XS
23
5.4 Support protocol NucleoSpin® RNA XS: rDNase digestion in the eluate
26
Appendix
28
6.1 Troubleshooting
28
6.2 Ordering information
32
6.3 References
33
6.4 Product use restriction / warranty
33
®
6
MACHEREY-NAGEL – 01 / 2010, Rev. 04
3