Download DNA Database GeneMapper ID Procedure

Procedure for GeneMapper® ID
DNA Database Section
Issued by DNA Database Forensic Scientist Manager and DNA Technical Leader
Version 2
Effective Date: 04/18/2014
5.2.3
Select “File” and “Add samples to project.”
5.2.4
Locate the run folder containing the samples to be added to the project under the “Files”
tab. Highlight the run folder (or select the specific samples to be analyzed) and select
“Add to List.” The file(s) then appears on the right-hand side. Select “Add.”
5.2.5
In the Samples pane, the category “Sample Type” shall have the samples labeled as
follows when amplified with Identifiler™:
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5.2.6
The remaining categories shall be filled out as follows:
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5.2.7
Ladder: Allelic Ladder
9947A (Positive Amplification Control): Positive Control
Negative Amplification Control: Negative Control
All other samples shall be “Samples”
Note: Sample File names appear by well number (i.e., A12) followed by the sample
name as entered by the DNA Database Forensic Scientist prior to electrophoresis.
Also, until the samples are analyzed, the “Status” icon to the left of the Sample File
name has the unanalyzed icon (
) present next to it. If no “Status” category or
icons are present, verify that the table setting (in top center of the main GMID
window) is set to “AmpFLSTR Table.”
Specimen Category: no export
Analysis Method: G5_Advanced or NCSBI Robot (for Robot runs)
Panel: Identifiler_v2
Size Standard: GS500LIZ_Advanced
Instrument Type: ABI3130, or similar (note: automatically filled in by the software
and instrument)
Once all samples and categories are properly labeled and identified, select the “Analyze”
icon ( ) to analyze the data. When prompted, create a project name for the set of
samples and select “Save.”
5.2.7.1 Note: GMID auto-saves data after every 10 samples have been processed.
5.2.7.2 Note: The Genotypes tab becomes available in the main GMID window once
analysis is complete.
5.2.7.3 Note: If any sample displays a solid red “stop” sign icon ( ) on the right-hand
side of the GMID window, assess the data as described in 5.3.
5.2.8
Analysis and Plot Settings
5.2.8.1 Access the Analysis Method Editor by selecting “Analysis” from the menu in the
main GMID window, and then select “Analysis Method Editor.” Note that the
user must have a single sample in the main GMID window selected in order to
access the Editor.
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