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,*>;@OO&,N)B*)N><@=&!gNQR)C+
(Y7D?F@)V
B*>;@OO&l&[.,&m&(,]&h&'&&&&&&(1)
Having 3 controls (NC, PC, HC), supposing to calculate the Cut-off
according to the optical density values average of the negative and
positive controls:
[NC = negative control ]
[PC = positive control ]
[HC = high positive control]
Considering that formula (1) can be written as following:
B*>;@OO = NC / 2 + PC / 2
comparing this formula with the general one provided by the instrument:
B*>;@OO = a ' C1 + b ' C2 + c (2)
it is possible to calculate the cut-off value as required by the
method, assigning to the coefficients “a”, “b” and “c” the following
values :
N&= 0, 5 S&=&0, 5
B&=&0;
The negative control has to be positioned in C1 position and the
positive control in C2 position.
The high positive control may be positioned in any other position.
(Y7D?F@)]
B*>;@OO&l&.,&m&`&'
'&(,
( K = Factor)
In this case the instrument calculates the cut-off as indicated by the
method, using the formula (2) and by choosing the following values
for the coefficients:
N&= 1
S&= K
B&= 0;
N=K
S&=&0
&&B&= 0;
The negative control has to be positioned in C1 position and the
positive control in C2 position.
(Y7D?F@)\
B*>;@OO&l&(,&'
'&`
( K = Factor )
In this case the instrument calculates the cut-off according to the
method requirements using the formula (2) and by choosing the
following values for the coefficients:
The positive control has to be positioned in C1 position .
(Y7D?F@)^
B*>;@OO&l&`
( K = constant )
In this case the instrument calculates the cut-off according to the
method requirements, using the formula (2) and by choosing the
following values for the coefficients:
N=0
S&=&0
B&= K.
(Y7D?F@)[
44/74
/PC&B*>;@OO&<+&NVN<)NS)C
[NC = negative control ]
[CO = Cut-off ]
[PC = positive control ]
Having 3 controls (NC, CO, PC), supposing to put the Cut-off in C2
position, choose the following values for the coefficients:
N=0
S&=&1
B&= 0.
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