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Contents Kit Contents 4 Shipping and Storage 4 Product Use Limitations 4 Product Warranty and Satisfaction Guarantee 5 Technical Assistance 5 Safety Information 5 Product Specifications 6 Quality Control 7 Introduction 8 Equipment and Reagents to Be Supplied by User 10 Protocols PCR Using PyroMark PCR Master Mix 11 PCR Using PyroMark PCR Master Mix and Q-Solution 15 Troubleshooting Guide 20 Appendix A: Starting Template 25 Appendix B: Assay and Primer Design, Handling and Storage of Primers 26 Appendix C: Control of Contamination 29 Ordering Information 31 PyroMark PCR Handbook 05/2009 3