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Download - 1 - Chapter: DNA Based Testing Section: Application - ASHI-U
Transcript
One Lambda LABType SSO Plate Layout 1 2 3 4 5 6 7 8 9 10 11 A B C D E F G H Pos QC ID:_____________________ Batch File Name:_________________________ Tech Name:____________________ PCR Set Up Denaturation/Hybridization/Labeling (per 1 sample per allele) (per 1 sample) 13.8 ul D-Mix Denaturation buffer - 2.5 ul SAPE stock - 0.5 ul 4ul Primer Neutralization buffer - 5 ul SAPE buffer - 49.5 2 ul DNA Hybridization buffer- 34 ul Bead Mixture - 4 ul 0.2 ul Taq Wash buffer - 480 ul - 32 - 12
