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SG500 instructions
The Hoefer® SG500 gradient maker is designed
for producing linear gradients of aqueous
solutions ranging in volume from 100–500 ml.
The SG500 can be used to create convex and
concave exponential gradients with the addition
of a one-holed rubber stopper, a piece of rigid
tubing and a piece of flexible tubing. The unit
is fabricated from cast acrylic plastic sheet and
tubing. It is suitable for casting acrylamide pore
gradient gels and delivering salt gradients for
low pressure chromatography systems.
reservoir (back) chamber
mixing (front) chamber
Fig 1. Hoefer SG500
gradient maker.
Included but not shown:
Hose clamps (4)
outlet connector
(4 mm ID tubing)
slide stop
push-pull valve
connector channel
• p1