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tmd012.0508.qxp 9/16/2008 3:48 PM Page 55 IX.H. Composition of Buffers and Solutions 10% ammonium persulfate Add 0.05g of ammonium persulfate to 500μl of deionized water. Blue Dextran Loading Solution 88.25% 15mg/ml 4.1mM formamide blue dextran EDTA (pH 8.0) ethidium bromide solution (10mg/ml) 1.0g ethidium bromide Dissolve ethidium bromide in 100ml of deionized water. Wrap in aluminum foil or transfer the solution to a dark bottle, and store at room temperature. ! Caution: Ethidium bromide is a powerful mutagen. Wear gloves when working with the dye, and wear a mask when weighing it. ★R 10X Buffer Gold ST★ 500mM 100mM 15mM 1% 2mM 1.6mg/ml KCl Tris-HCl (pH 8.3 at 25°C) MgCl2 Triton® X-100 each dNTP BSA TAE 50X buffer (pH 7.2) 242g 57.1ml 100ml Tris base glacial acetic acid 0.5M EDTA stock Add Tris base and EDTA stock to 500ml of deionized water. Add glacial acetic acid. Bring the volume to 1 liter with deionized water. TBE 10X buffer 107.8g 7.44g ~55.0g Tris base EDTA (Na2EDTA • 2H2O) boric acid Dissolve Tris base and EDTA in 800ml of deionized water. Slowly add the boric acid, and monitor the pH until the desired pH of 8.3 is obtained. Bring the final volume to 1 liter with deionized water. TE-4 buffer (10mM Tris-HCl, 0.1mM EDTA [pH 8.0]) 2.21g 0.037g Tris base EDTA (Na2EDTA • 2H2O) Dissolve Tris base and EDTA in 900ml of deionized water. Adjust to pH 8.0 with HCl. Bring the final volume to 1 liter with deionized water. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Revised 5/08 Part# TMD012 Page 55
