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5/3/2012
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7.B. GeneMapper® ID Analysis Software
Symptoms
Alleles not called
5685TA
Off-ladder alleles
Causes and Comments
To analyze samples with GeneMapper ® ID software, the
analysis parameters and size standard must both have “Basic
or Advanced” as the analysis type. If they are different, an
error is obtained (Figure 11).
An insufficient number of ILS 600 fragments was defined. Be
sure to define at least one ILS 600 fragment smaller than the
smallest sample peak or allelic ladder peak and at least one
ILS 600 fragment larger than the largest sample peak or allelic
ladder peak.
Run was too short, and larger peaks in ILS were not captured.
Not all ILS 600 peaks defined in the size standard were
detected during the run.
• Create a new size standard using the internal lane standard
fragments present in the sample.
• Re-run samples using a longer run time.
An allelic ladder from a different run than the samples was
used. Re-analyze samples with an allelic ladder from the
same run.
The GeneMapper® ID software requires that the allelic ladder
be imported from the same folder as the sample. Be sure that
the allelic ladder is in the same folder as the sample. Create a
new project and re-analyze, as described in Section 6.B or 6.C.
Panels text file selected for analysis was incorrect for the STR
system used. Assign correct panels file that corresponds to the
STR system used for amplification.
The allelic ladder was not identified as an allelic ladder in the
Sample Type column.
The wrong analysis type was chosen for the analysis method.
Be sure to use the HID analysis type.
The internal lane standard was not properly identified in the
sample. Manually redefine the sizes of the size standard
fragments in the sample.
Figure 11. The error message that appears in the GeneMapper® ID software when the
analysis parameters and size standard have different analysis types.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TMD018
Page 44
Printed in USA.
Revised 5/12