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CHAPTER 1 Eukaryotic Target Preparation 4. Mix thoroughly and spin down to collect the liquid at the bottom of the tube. 5. Add 2 µL of the Second Dilution to 18 µL of Poly-A Control Dil Buffer to prepare the Third Dilution (1:10). 6. Mix thoroughly and spin down to collect the liquid at the bottom of the tube. 7. Add 2 µL of this Third Dilution to 5 µg of sample total RNA. Eukaryotic The First Dilution of the poly-A RNA controls can be stored up to six weeks in a nonfrost-free freezer at -20°C and frozen-thawed up to eight times. 2.1.15
