1
Download GeneChip Expression Analysis Technical Manual
Transcript
SECTION 2 Eukaryotic Sample and Array Processing Antibody Solution Table 2.3.2 Antibody Solution Mix Components Volume Final Concentration 2X Stain Buffer 300.0 µL 1X 50 mg/mL BSA 24.0 µL 2 mg/mL 10 mg/mL Goat IgG Stock 6.0 µL 0.1 mg/mL 0.5 mg/mL biotinylated antibody 3.6 µL 3 µg/mL 266.4 µL — DI H20 Total Volume 600 µL Table 2.3.3 Fluidics Scripts for 11 µm Feature Size Eukaryotic Arrays* Format 49 64 100 169 400 Using GeneChip® IVT Labeling Kit EukGE-WS2v5 EukGE-WS2v5 Midi_euk2v3 Mini_euk2v3 Micro_1v1 Using all other labeling kits EukGE-WS2v4 EukGE-WS2v4 Midi_euk2v3 Mini_euk2v3 Micro_1v1 * When using the Fluidics Station 450 or 250, add _450 at the end of the fluidics script’s name. Table 2.3.4 Fluidics Scripts for ≥ 18 µm Feature Size Eukaryotic Arrays* Format 49 64 100 169 400 Using GeneChip® IVT Labeling Kit EukGE-WS2v4 EukGE-WS2v4 Midi_euk2v3 Mini_euk2v3 Micro_1v1 Using all other labeling kits EukGE-WS2v4 EukGE-WS2v4 Midi_euk2v3 Mini_euk2v3 Micro_1v1 * When using the Fluidics Station 450 or 250, add _450 at the end of the fluidics script’s name. 2.3.10
