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Table of Contents Pump Information..........................................................................................................................25 Power Up Running ........................................................................................................................26 Syringe Loading ..................................................................................................................................27 Changing the bracket for small (<30ml) vs large (>30ml) syringes: ......................................28 Using The PHD ULTRA™ ....................................................................................................29 Pump Operation Overview ..............................................................................................................29 Overview of Methods ........................................................................................................................29 Method Types ......................................................................................................................................30 EZ PRO™ Quick Start Methods ..................................................................................................30 EZ PRO™ Preloaded Methods......................................................................................................30 EZ PRO™ User-Defined Methods ..............................................................................................30 Running The Pump ............................................................................................................................31 Controlling The Pump ..................................................................................................................31 Basic Method Operations..............................................................................................................31 Viewing Current Method Parameters ....................................................................................31 Syringe Selection ........................................................................................................................32 Syringe Gang Operation............................................................................................................33 Controlling The Pump Using A Stored Method ......................................................................35 Using an EZ PRO™ Quick Start Method ........................................................................................39 Basic Operation ..............................................................................................................................39 Quick Start Method Variables ......................................................................................................40 Infuse Rate Select/Withdraw Rate Select ..............................................................................40 Target Volume/Time Select ....................................................................................................41 Using an EZ PRO™ Preloaded/User-Defined Method ................................................................42 Preloaded/User-Defined Method Workflow ............................................................................43 Setting A Time Delay For Method Operation ..........................................................................45 Creating an EZ PRO™ New Method................................................................................................46 Modifying a Preloaded/User-Defined Method..........................................................................48 Changing the Order of Steps in a Method ............................................................................48 Deleting a Step From a Method ..............................................................................................48 Duplicating a Step In a Method ..............................................................................................49 Defining Method Step Parameters ..................................................................................................49 Pump Operation Profiles ..............................................................................................................50 Constant Rate Profile ................................................................................................................51 Ramp Profile ..............................................................................................................................52 Stepped Profile ..........................................................................................................................54 Pulse Profile ................................................................................................................................56 Publication 5419-002-Rev-E Harvard Apparatus PHD ULTRA™ Syringe Pump Series User’s Manual 3