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1 System Description
Optical System
The optical system includes a xenon flash lamp, the monochromators, excitation
bandpass filters, emission cut-off filters, and a PMT detector.
There are a number of cables and fibers that exit the optics bench and enter the read
chamber. They are the excitation fiber (thin and black, has a collar and pins), emission
fiber (fatter, with attached electrical cord), electrical connector to the read head (black
with brass fitting).
Caution: Optical fibers are very fragile, especially the excitation fiber. Handle cables with
extreme care. Do not flex, twist, bend, or stretch the optical cables.
Excitation
Monochromator
Collimation
Mirror
Emission
Monochromator
Photomultiplier
Tube
Microplate
Flash Lamp
Flash Lamp
Elliptical Focusing
Mirrors
Optical System
Overview of Optical System
1 The excitation light source is a xenon-arc flash lamp. (Note that the lamp is off when
luminescence mode is selected.)
2 The light passes through a band-pass filter which reduces the amount of stray light to the
excitation monochromator.
3 The holographic diffraction grating monochromator selects the desired excitation
wavelength.
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FlexStation II Operator’s Manual – Rev. D