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Over ĚŝŐĞƐƟŽŶ
Wash the cells with PBS 2-3 ƟŵĞƐ to reŵove
ƐĞƌƵŵ prior to tryƉƐŝŶŝzaƟoŶ (seruŵ will ŝŶŚŝďŝƚ
the fuŶcƟŽŶ of trLJƉƐŝŶͿ͘
Cell aging
Cells show
spontaneous
ĚŝīĞƌĞŶƟĂƟŽŶ
/ŶĞīĞĐƟǀĞ
ŝŶĚƵĐƟŽŶ of Đell
ĚŝīĞƌĞŶƟĂƟŽŶ
CoŶtrol the diŐĞƐƟoŶ ƟŵĞ͘
The passagiŶŐ Ɵŵe is ŶŽt
appropriate
The cells should ďĞ ƐƵďĐƵůƚured ǁŚĞŶ
rĞĂĐŚŝŶŐ 80-90% cŽŶŇƵĞŶĐLJ iŶ order to avoid
coŶtact ŝŶŚŝďŝƟŽŶ͘
DMSO is ŶŽt coŵpletely
ƌĞŵŽǀĞĚ ĚƵƌŝŶŐ cell recovery
Wash the cells with pre-warŵed ŵĞĚŝƵŵ 2-3
ƟŵĞƐ ĚƵƌŝŶŐ recovery͘
ŝīĞƌĞŶƟĂƟŽŶ ƌĞĂŐĞŶƚƐ Ŷeed
to ďĞ opƟŵized
Cell passage is too high
Use CyagĞŶ tailor-ŵĂĚĞ diīereŶƟaƟŽŶ
ŵĞĚŝĂ͘
Use cells at a low ŽƌŝŐŝŶĂů passage ŶƵŵďĞƌ͘
Related Products
Product
Catalog Number
0.25%Trypsin-0.04%EDTA
TEDTA-10001
Phosphate-Buffered Saline (1xPBS)
PBS-10001
OriCellTM Wistar Rat Adipose-Derived Mesenchymal
Stem Cells
RAWMD-01001
OriCellTM Rat Adipose-Derived Mesenchymal Stem Cell
Growth Medium
RAXMD-90011
OriCellTM Mesenchymal Stem Cell Osteogenic
Differentiation Medium
GUXMX-90021
OriCellTM Mesenchymal Stem Cell Adipogenic
Differentiation Medium
GUXMX-90031
OriCellTM Mesenchymal Stem Cell Chondrogenic
Differentiation Medium
GUXMX-90041
OriCellTM NCR Protein-Free Cryopreservation Medium
NCPF-10001
REFENRENCES
JM Gimble, and F Guilak. (2003) Adipose-derived adult stem cells: isolation,
characterization, and differentiation potential. ISCT 5: 362-369.
Cyagen Biosciences reserves all rights on the technical documents of its OriCellTM cell
culture products. No part of this document may be reproduced or adapted for other
purposes without written permission from Cyagen Biosciences.
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KYUHO江坂ビル8階
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http://www.dspbio.co.jp/　[email protected]
IMPI0056A3 RAWMD-01001
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