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SFM-3/4 User’s Manual (ver 1.1) Figure 60 – SFM Syringe Filling Procedure Panel 1 Panel 2 Panel 3 R R R C C C Panel 4 Panel 5 R R C C 12.4 SFM Cleaning and Storage After each day’s experiments the SFM should be cleaned. A thorough cleaning of the SFM will ensure that it has a long functional life and diminish any chance of sample contamination for the next user of the instrument. The procedure below is the recommended daily cleaning procedure to be done before shutting off the instrument. 1) Remove and remaining samples or buffer from the syringes. 2) Wash the syringes and flow lines 2 – 3 times with water. This is done by filling each syringe with water to a volume at least equal to the sample volume used for experiments. With the syringe valve handles set to (C), empty the syringes completely. Since the liquid will exit via the exit valve, it will wash the flow lines and exit valve as well as the syringes. 3) Wash the syringes and flow lines one time with 70 – 100% ethanol. Use the same procedure as in step 2). 12–76