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Download kit manual - Alere Technologies GmbH
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www.alere-technologies.com www.alere.com REAGENTS AND DEVICES Kit Components, Storage and Stability All reagents are provided in surplus (see below). If necessary, all components may be ordered separately. Please refer to the catalogue reference numbers (Cat#) at the end of this manual. For pricing please contact your local representative or our customer service, respectively. The expiry date can be found on each bottle and on the outer packaging. All components were tested for short term shipment (< 1 week) at ambient temperature (< 37 °C). The assay components with limited stability are D1 and C3. The other kit components proved to be stable six months after post expiry. Cell Lysis (optional order) A1: Lysis Buffer (Cat# 245101000) Store at 18 to 28 °C (ambient temperature). Surplus: 50 %. A2: Lysis Enhancer (lyophilised, Cat# 245102000) Store at 18 to 28 °C (ambient temperature). Centrifuge A2 tubes shortly prior to opening. Add 200 µl Buffer A1 to Lysis Enhancer before use. Mix well and store for less than 1 week at 2-8 °C. Sufficient for 96 isolations. DNA Labelling and Amplification B1+: Labelling Buffer, Store at 2-8 °C. Surplus: 40 %. B2: Labelling Enzyme, Store at 2-8 °C. Surplus: 100 %. B3Ec01: lyophilised Labelling Primermix, two tubes, dilute each in 70 µl molecular grade water. Store at -20 °C. Surplus: 50% E. coli SeroGenoTyping AS-1 Kit 05_16_04_0016_V01_Manual E. coli SeroGenoTyping AS-1 Kit 4
