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Chapter 5 Experiments and Results Extra Peaks in the electropherogram 5 Table 3 Marker-specific stutter filter percentages for Yfiler® Plus Kit loci Locus designation % Minus stutter† % Plus stutter† %Minus-2 /%Plus-2 nt stutter† DYS576 15.15 3.38 ‡ DYS389I 9.16 3.45 ‡ DYS635 13.38 3.3 ‡ DYS389II 18.79 3.73 ‡ DYS627 15.18 2.62 2.71 DYS460 11.65 4.27 ‡ DYS458 15.31 2.52 ‡ DYS19 12.68 3.72 10.1/3.42 YGATAH4 11.53 2.27 ‡ DYS448 4.68 2.29 ‡ DYS391 9.99 3.41 ‡ DYS456 15.36 3.74 ‡ DYS390 13.58 3.51 ‡ DYS438 5.86 2.76 ‡ DYS392 16.94 11 ‡ DYS518 25.5 4.85 ‡ DYS570 15.65 2.88 ‡ DYS437 8.13 1.65 ‡ DYS385 18.32 3.7 ‡ DYS449 23.24 4.2 ‡ DYS393 14.07 4.95 ‡ DYS439 9.89 3.39 ‡ DYS481 28.55 5.59 9.55 DYF387S1 15.71 NA ‡ 12 4.6 1.88 DYS533 † The stutter filters are displayed as percentages in the GeneMapper ID-X Yfiler_Plus_Stutter.txt file. ‡ Undetermined IMPORTANT! The values in Table 3 were determined by Life Technologies during the developmental validation studies. We recommend that laboratories perform their own internal validation studies to determine the appropriate values to use. Yfiler® Plus PCR Amplification Kit User Guide 71
