Download AmpFLSTR Yfiler PCR Amplification Kit User`s Manual (PN 4358101C)

Chapter 6 Experiments and Results
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Figure 6-13 Multiplex amplification of DNA samples incubated
with DNAseI. The top panel corresponds to 1 ng of DNA with no
DNAseI added; panels 2-6 correspond to 2 ng of DNA incubated
with DNAseI for 1, 2, 4, 8, and 12 minutes respectively.
Effect of
Inhibitors
Heme compounds have been identified as PCR inhibitors in DNA
samples extracted from bloodstains (DeFranchis et al. 1988; Akane et
al., 1994). It is believed that the inhibitor is co-extracted and copurified with the DNA and subsequently interferes with PCR by
inhibiting polymerase activity.
To examine the effects of hematin on the amplification results
obtained by the AmpFlSTR Yfiler kit, DNA samples were amplified
using the AmpFlSTR Yfiler kit reagents (including the BSAcontaining PCR reaction mix) in the presence of increasing
concentrations of purified hematin. The concentrations of hematin
used were 0 µM, 10 µM, 12 µM, 16 µM, 20 µM, and 24 µM. No
preferential amplification was observed in the presence of increasing
amounts of hematin.
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AmpFlSTR Yfiler PCR Amplification Kit User’s Manual
August 7, 2006 8:20 am, ExperimentsResults.fm