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Flocel
Table of Contents
Minimum System Requirements ____________________________________________3
Package Contents _______________________________________________________3
Customer Support _______________________________________________________3
Introduction ____________________________________________________________4
The Nine Basic Steps in Setting up The Flocel System for Use ____________________4
Flocel System Setup _____________________________________________________5
Flocel System Hardware Details ____________________________________________6
DIV-BBB Cartridge _______________________________________________________6
Pulsatile Pump (Quad Pump) ______________________________________________6
TEER Measurement Unit __________________________________________________7
Flocel Measurement Software-LabVIEW Application Program _____________________8
Flocel Software Installation ________________________________________________8
Flocel System Operation ___________________________________________________9
Cartridge Setup Menu ___________________________________________________10
Main Screen Details _____________________________________________________11
Viewing Data __________________________________________________________16
Appendix I – System Specifications ________________________________________17
Appendix II – Flocel USB Driver Installation Guide _____________________________18
Appendix III – Theory of Operation _________________________________________22
Appendix IV – Sterilization of Flocel System Components _______________________24
Appendix V – Preparing the Flocel DIV-BBB Cartridge for Cell Culture _____________25
Setting up the Flocel DIV-BBB Cartridge ____________________________________26
Pre-coating DIV-BBB cartridges ___________________________________________26
Loading Endothelial Cells ________________________________________________27
Daily Maintenance Schedule ______________________________________________28
Expected Impedances ___________________________________________________28
Flow Rate and Shear Stress ______________________________________________28
FAQs ________________________________________________________________29
Cell Extravasation Testing ________________________________________________30
Note: images may not show how current product looks as some minor details have been updated.
Flocel Inc. and DIV-BBB are trademarks of Flocel Inc., Cleveland, OH.
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