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Introduction
Each year pharmaceutical companies spend millions of dollars to develop and test drugs using in
vitro models of the blood brain barrier (BBB). Flocel’s Dynamic In Vitro Blood-Brain Barrier (DIVBBB) Model is practical and more accurately reflects true BBB characteristics. The DIV-BBB
model works with the Trans Epithelial Electrical Resistance (TEER) measurement system which
provides a quick and easy evaluation of the integrity of the Blood-Brain Barrier model by measuring
the impedance across its threshold.
The Flocel System enables more accurate pharmacokinetic and toxicological studies and reflects
in vivo blood-brain barrier properties. It mimics important endothelial cell-astrocyte interaction and
does so by use of its dynamic system.
The researcher can utilize real human cells in this system. Tighter junctions between cells are
formed within the cartridge’s dynamic model, which cannot be obtained by other existing static
models.
This system will substantially reduce drug development cost and experimental time as four
independent tests can be run simultaneously using the Flocel System.
The Nine Basic Steps in setting up the Flocel System for use:
1. Check Package Contents (Pg. 3)
2. Flocel System set up (Pg. 5)
3. Flocel Software Installation (Pg. 8)
4. USB driver installation (Pg. 18)
5. Sterilization of the Flocel System (Pg. 24)
6. Preparing the Flocel System for cell culture (Pg. 25)
7. Loading Endothelial Cells into the Flocel System (Pg. 27)
8. Open Flocel program and set experiment parameters (Pg. 9)
9. Viewing Data (Pg. 16)
Flocel Inc. and DIV-BBB are trademarks of Flocel Inc., Cleveland, OH.
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