Download Perkin Elmer Lambda 12-14-14P Operation manual

Concentration 2
Determining the Measurement Wavelengths (Peak areas)
1) To determine the wavelengths:
a) Record the spectrum of the sample {see SCAN method, page
5-8).
b) Select a strong peak and note the wavelength at its start
(WAV. MAX) and end (WAV. MIN).
A
A
...or...
WAV.MIN
WAV. MAX
WAV.MIN
WAV. MAX
Determining the Measurement Wavelengths (2nd derivative)
1) To determine the wavelengths:
a) Record the spectrum of the sample {see SCAN method, page
5-8).
b) Select a strong peak and note the wavelength at its start
(WAV. MAX) and end (WAV. MIN).
c) Record the spectrum of the same sample using the 2nd
derivative (D2 mode) over the wavelength range
determined in step b above.
d) From the 2nd derivative spectrum determine the
wavelength at the peak maximum and peak minimum.
Use these values for CALC.WAV 1 and CALC. WAV 2.
D2
WAV.MIN
B2161.20
Release 2.0
CALC.WAV 2
(Peak minimum)
CALC.WAV 1
(Peak maximum)
WAV. MAX
5-19