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Chapter 4 | Water Analysis Test Menu 7. Add the reagent or reagents to the vial. Close the vial tightly with the cap and mix the contents. Wipe the exterior of the vial. 8. Place the vial into the holder in the sample chamber and close the chamber door. 9. Press the Measure Rgnt Blank function key to measure the reagent blank. 10. Open the sample chamber door and remove the vial from the holder. 11. Empty and rinse the vial and then fill the vial with sample. Add the reagent or reagents to the vial. Close the vial tightly with the cap and mix the contents. Wipe the exterior of the vial. 12. Place the vial into the holder in the sample chamber and close the chamber door. 13. Press the Measure Sample function key to display the results. Thermo Scientific Orion AquaMate Spectrophotometer User Manual | 43