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Chapter 5 | Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate
ACR007 Nitrate Reaction Tube Test
Chromotropic Acid Method
1 – 30 mg/l N (Nitrate as Nitrogen)
1. Load and run the ACR007 method.
2. Open one 16 mm reaction vial (Reagent A) and add 1 ml of deionized water (this is the
blank vial).
3. Open a second 16 mm reaction vial (Reagent A) and add 1 ml of sample (this is the
sample vial).
4. Add the contents of one Nitrate Chromotropic Powder Pack straight from the foil into each
vial.
5. Close the vials tightly with the caps and invert gently about 10 times to mix contents. Some
solids may not dissolve. Wipe the exteriors of the vials.
6. Wait for a reaction period of 5 minutes.
7. Place the blank vial into the holder in the sample chamber and close the chamber door.
8. Press the Measure Blank function key to measure the blank.
9. Open the sample chamber door. Remove the blank vial from the holder.
10. Place the sample vial into the holder in the sample chamber and close the chamber door.
11. Press the Measure Sample function key to display the result in mg/l nitrate as N.
Notes:
Thermo Scientific
•
Some solids may not dissolve.
•
Conversion: mg/l NO3 = mg/l N x 4.43
Orion AquaMate Spectrophotometer User Manual | 89