Download A Parylene Real Time PCR Microdevice

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4.5 Device Performance
4.5.1 Real Time Polymerase Chain Reaction Components
The same RTPCR mixture was used as in the previous version. Please see chapter 3 for
more details.
4.5.2 Thermal Cycling Protocol
The same thermal recipe was used as in the previous version. Please see chapter 3 for
more details.
4.5.3 Optical Detection Protocol
The same optical protocol was used except a 4X objective (instead of 10X) was used, at
a shutter speed of 350 ms. The same normalization scheme was also used. Please see
Chapter 3 for details.