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TABLE OF CONTENTS TABLE OF CONTENTS SAFETY SECTION 1. Chapter 1.0 1.1 1.2 1.3 1.4 1.5 SYSTEM OVERVIEW Introduction ...............................................................................................................1-1 Overview .....................................................................................................................1-1 Features ......................................................................................................................1-2 Unpacking ...................................................................................................................1-3 System Configurations ................................................................................................1-4 Quick Start Procedure.................................................................................................1-5 Chapter 2.0 Description of System Components .......................................................................2-1 2.1 Controller and USB Bitbus Communicator .................................................................2-2 2.1.1 Controller........................................................................................................2-2 2.1.2 USB Bitbus Communicator ............................................................................2-5 2.2 Workstation .................................................................................................................2-6 2.3 BioLogic Maximizer™ Valve System ..........................................................................2-11 2.4 Mixers..........................................................................................................................2-18 2.4.1 MX-1 Mixer.....................................................................................................2-18 2.4.2 Maximizer Mixer .............................................................................................2-19 2.4.3 Changing Mixer Capacity ...............................................................................2-20 2.5 Detection Systems ......................................................................................................2-21 2.5.1 UV Detector....................................................................................................2-21 2.5.2 Conductivity Monitor.......................................................................................2-23 2.5.3 BioLogic QuadTec™ UV/Vis Detector............................................................2-23 2.5.4 pH Monitor......................................................................................................2-25 2.5.5 Other Detectors..............................................................................................2-25 2.6 Valves..........................................................................................................................2-26 2.6.1 AVR7-3 Sample Inject Valve ..........................................................................2-26 2.6.2 AVR9-8 Stream Select Valve .........................................................................2-29 2.6.3 SV5-4 Buffer Select and Automated Sample Loading Valve .........................2-31 2.6.4 SVT3-2 Diverter Valve....................................................................................2-33 2.7 Fraction Collectors ......................................................................................................2-35 2.7.1 BioFrac™ Fraction Collector ..........................................................................2-35 2.7.2 Model 2110 Fraction Collector .......................................................................2-37 2.7.3 Model 2128 Fraction Collector .......................................................................2-38 2.7.4 Generic Fraction Collectors............................................................................2-39 2.8 Sample Loading Options.............................................................................................2-40 2.8.1 DynaLoop Large Volume Sample Injection Loop...........................................2-40 2.8.2 Model EP-1 Econo Pump...............................................................................2-42 2.8.3 EGP Econo Gradient Pump ...........................................................................2-43 2.8.4 Other Gradient Pumps ...................................................................................2-44 2.9 System Peripherals .....................................................................................................2-45 2.9.1 System Rack ..................................................................................................2-45 2.9.2 Starter Kit .......................................................................................................2-47 2.9.3 Fittings Kit, including Tubing Kit .....................................................................2-47 2.9.4 Fittings Tightener............................................................................................2-48 2.9.5 Backpressure Regulator.................................................................................2-49 2.9.6 Signal Import Module (SIM) ...........................................................................2-50 2.9.7 Pump Kits.......................................................................................................2-51 iii