Download MACSQuantify™ Software guide Basics of flow cytometric analysis

positive fraction is shown. A total of 1.56x105 viable monocytes were enriched from the
original PBMC fraction, which accounted for an enrichment rate of 69.7%. The analysis
template “CD14” automatically generated the “gating” strategy and associated statistics.
8.8 Reading reagents with the code reader in Express
mode
The 2D code reader (“barcode reader”) is used to scan reagent vials. Reagent vials are
automatically recognized and logged by the MACSQuantify Software. WARNING! Read
the chapter 1 of the user manual before using the 2D Code Reader.
To scan reagents perform the following:
1) Click the activate code reader icon,
. The code reader will being blinking.
2) Present the reagent vial in front of the 2D code reader. Ensure the 2D code is
facing the blinking code-reader light. The optimal reading distance is 0.5–2.5
cm from the code reader cover, tilt the vial as depicted in Figure 8.18.
Figure 8.18 Scanning a reagent using the MACSQuant Instrument 2D code reader.
3) Scanned reagents are reported by a MACSQuantify Software dialog box.
Note:
When scanning MACSQuant Calibration Beads the instrument will
Note:
When scanning MACS Reagents the MACSQuantify Software will
Note:
Contact your administrator if the code reader fails to recognize a
prompt to initiate the calibration procedure:
prompt the user to place the vial(s) on the MACS Reagent Rack.
reagent vial.
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