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CARPHA Laboratory Procedure Manual
Section: QSE 05 – Process Control
Title: Laboratory User Manual
Page 29 of 58
Effective date: 01.07.2013
Doc. No: GUL-Q05-001
Review date: 01.07.2014
Edition: 1
B.1.3 ASPIRATES
PROCEDURE
STEP
ACTION
1
 Nasopharyngeal secretions are aspirated through a catheter connected to a mucus trap
and fitted to a vacuum source.
2
 The nasal aspirates are collected by introducing a few ml of saline into the nose with a
syringe fitted with a fine tubing or catheter.
3
 The catheter is inserted into a nostril parallel to the palate.
 The vacuum is then applied and the catheter is slowly withdrawn with a rotation motion.
4
 Mucus from the other nostril is collected with the same catheter in a similar manner.
5
 After mucus has been collected from both nostrils, the catheter is flushed into a screw
cap vial with 3 ml viral transport media
6
 Label the vial with patient’s name type of specimen and date of collection
B.2.0 LOWER RESPIRATORY TRACT SPECIMENS
B.2.1 SPUTUM
PROCEDURE
STEP
1
ACTION
 Instruct patient to take a deep breath and cough up sputum directly into a wide mouth
sterile container.
 Avoid saliva or postnasal discharge.
 Minimum volume should be about 1 ml
2
 Label the specimen containers
3
 Complete the laboratory request forms