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2) Click the Experiment tab and select the Chill 5 tube rack format from the Rack pull-down menu. Figure 3.23 Selecting Chill 5 tube rack using the Experiment tab Note: The Chill 15 and Chill 50 rack do not need to be calibrated separately. The calibration of the Chill 5 will also ensure for the appropriate calibration of the Chill 15 and Chill 50 rack. Note: If the Chill rack 5 is not selected the following error will be reported: “Specified rack must be specified in the experiment settings”. 3) Load a Chill 5 rack with empty 5 mL tubes in the position D6 (see Figure 3.24). Figure 3.24 Position D6 (marked ‘X’) is used for calibration of Chill Rack 5. 4) Place the loaded rack onto the MACS® MiniSampler. 5) Click Start calibration. The needle arm will automatically insert the needle into a tube located at rack position D6. Figure 3.25 Calibration of the uptake unit is underway. The yellow closed circle indicates that the needle arm may be freely moved for manual calibration. 50