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General Guidelines, Continued Appropriate Controls When performing RNAi analysis, it is important to include proper positive and negative controls to help evaluate your results. The BLOCK-iT™ Fluorescent Oligo and Stealth™ RNAi Negative Control Duplex are included in the BioModule™ Transfection and Control Unit for your convenience. Use the BLOCK-iT™ Fluorescent Oligo as an indicator of transfection efficiency with Stealth™ RNAi or siRNA using any fluorescence microscope with a standard FITC filter set. Be sure to include your assay specific controls and controls for mock or untransfected cells. 13