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Table of Contents Table of Contents ................................................................................................................................................. iii Experienced Users Procedure ............................................................................................................................. v Kit Contents and Storage .................................................................................................................................... vi Introduction ................................................................................................................... 1 Overview.................................................................................................................................................................1 Description of Components..................................................................................................................................5 Experimental Overview ........................................................................................................................................8 Methods ....................................................................................................................... 10 General Guidelines ..............................................................................................................................................10 Transfecting Cells ................................................................................................................................................14 Expected Results ..................................................................................................................................................20 Troubleshooting ...................................................................................................................................................22 Appendix...................................................................................................................... 24 Technical Service..................................................................................................................................................24 Product Qualification ..........................................................................................................................................25 Accessory Products..............................................................................................................................................26 Purchaser Notification ........................................................................................................................................28 References .............................................................................................................................................................30 iii