Download cDNA Isolation Kit

Transcript
Table of Contents
1. Introduction -----------------------------------------------------------------------------2. List of Components -------------------------------------------------------------------3. Reagent and Equipment to Be Supplied by User -----------------------------4. Storage Conditions -------------------------------------------------------------------5. First-strand cDNA Synthesis -------------------------------------------------------6. PCR reaction for RACE
A. Primer design ----------------------------------------------------------------------B. PCR for 5’ and 3’-RACE ---------------------------------------------------------7. Isolation of Full-length target cDNA ----------------------------------------------8. Troubleshooting Guide --------------------------------------------------------------Appendix A. Expected Result of 5’-RACE -----------------------------------------Appendix B. Expected Result of 3’- RACE ----------------------------------------Appendix C. Isolation of Full-length cDNA from RACE Fragments ---------Related Products -------------------------------------------------------------------------Seegene’s Distributors -------------------------------------------------------------------
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