Download cDNA Isolation Kit
Transcript
Table of Contents 1. Introduction -----------------------------------------------------------------------------2. List of Components -------------------------------------------------------------------3. Reagent and Equipment to Be Supplied by User -----------------------------4. Storage Conditions -------------------------------------------------------------------5. First-strand cDNA Synthesis -------------------------------------------------------6. PCR reaction for RACE A. Primer design ----------------------------------------------------------------------B. PCR for 5’ and 3’-RACE ---------------------------------------------------------7. Isolation of Full-length target cDNA ----------------------------------------------8. Troubleshooting Guide --------------------------------------------------------------Appendix A. Expected Result of 5’-RACE -----------------------------------------Appendix B. Expected Result of 3’- RACE ----------------------------------------Appendix C. Isolation of Full-length cDNA from RACE Fragments ---------Related Products -------------------------------------------------------------------------Seegene’s Distributors ------------------------------------------------------------------- www.see-gene.com 4 7 7 7 8 10 11 12 16 17 17 17 18 19 3