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USER GUIDE
Countess™ II Automated Cell Counter
For brightfield applications
Catalog Number AMQAX1000
Publication Number MAN0014293
Revision A.0
For Research Use Only. Not for use in diagnostic procedures.
Online Specials
Information in this document is subject to change without notice.
DISCLAIMER
TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE
FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN
CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information
These products may be covered by one or more Limited Use Label Licenses. By use of these products, you
accept the terms and conditions of all applicable Limited Use Label Licenses.
© 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher
Scientific and its subsidiaries unless otherwise specified.
ii
Contents
About this guide ............................................................................................................ 3
1. Product information ................................................................................................ 5
Product description ......................................................................................................................................... 5
Instrument exterior components ................................................................................................................... 6
2. Getting started ........................................................................................................ 7
Installation ........................................................................................................................................................ 7
Load profile ...................................................................................................................................................... 9
3. Cell count and cell viability assays ........................................................................ 13
Prepare sample .............................................................................................................................................. 13
Count cells ...................................................................................................................................................... 14
4. Results .................................................................................................................. 16
View results .................................................................................................................................................... 16
Identify the cells counted ............................................................................................................................. 17
Graph count results ....................................................................................................................................... 18
Gate count results .......................................................................................................................................... 19
5. Save results .......................................................................................................... 20
Save count results .......................................................................................................................................... 20
6. Instrument settings ............................................................................................... 23
Overview ........................................................................................................................................................ 23
Software update ............................................................................................................................................. 24
Date/Time ...................................................................................................................................................... 25
7. Maintenance .......................................................................................................... 27
Instrument care .............................................................................................................................................. 27
Clean the Countess™ II Automated Cell Counter ..................................................................................... 28
Set nominal focus .......................................................................................................................................... 29
Appendix A: Troubleshooting...................................................................................... 31
Appendix B: Product specifications ............................................................................ 33
Technical specifications ................................................................................................................................ 33
Appendix C: Ordering information .............................................................................. 34
Countess™ II Automated Cell Counter ....................................................................................................... 34
Accessory products ....................................................................................................................................... 34
Appendix D: CSV file format........................................................................................ 35
CSV file format, explained ........................................................................................................................... 35
1
Appendix E: Safety ...................................................................................................... 36
Safety conventions used in this document................................................................................................. 36
Symbols on instruments ............................................................................................................................... 37
Safety labels on instruments ........................................................................................................................ 39
General instrument safety ............................................................................................................................ 40
Chemical safety .............................................................................................................................................. 41
Chemical waste safety................................................................................................................................... 42
Electrical safety .............................................................................................................................................. 43
Biological hazard safety ................................................................................................................................ 44
Safety and electromagnetic compatibility (EMC) standards ................................................................... 45
Documentation and support ....................................................................................... 46
Obtaining support ......................................................................................................................................... 46
2
About this guide
Audience
This user guide is for laboratory staff operating, maintaining, and analyzing data
using the Countess™ II Automated Cell Counter.
Revision history
Revision
Date
Description
A.0
August 2015
New user guide
™
User documentation The guides listed below are available for the Countess II Automated Cell
Counter.
Guide
Pub. no.
Countess II Automated Cell Counters User Guide
MAN0014293
Countess II and Countess II FL Automated Cell
Counters Quick Reference Card (QRC)
MAN0010826
™
™
™
Additional resources are available on the Countess™ Technical Resources page.
Go to www.thermofisher.com/countess to access protocols, application notes,
and tutorials.
Text and keyboard
conventions
Text and keyboard conventions used in this user guide are listed below. For safety
alert words and symbols used in this document, see page 4.
Convention
User attention
words
Use
Bold
Bold text indicates user action. For example:
Press More.

Right arrow symbol () indicates a menu choice, and
separates successive commands you execute or select from a
drop-down or shortcut menu. For example:
Select More  Adjust.
Two user attention words appear this document. Each word implies a particular
level of observation or action as described below.
Note: Provides information that may be of interest or help but is not critical
to the use of the product.
IMPORTANT! Provides information that is necessary for proper instrument
operation, accurate installation, or safe use of a chemical.
3
Safety alert words
Four safety alert words appear in this document at points where you need to be
aware of relevant hazards. Each alert word—IMPORTANT, CAUTION,
WARNING, DANGER—implies a particular level of observation or action, as
defined below:
IMPORTANT! – Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Except for IMPORTANT! safety alerts, each safety alert word in this document
appears with an open triangle figure that contains a hazard symbol. These hazard
symbols are identical to the hazard symbols that are affixed to the instruments
(see “Safety symbols” in Appendix E).
4
1. Product information
Product description
Product contents
The Countess™ II Automated Cell Counter is shipped with the components listed
below.
Component
Quantity
Countess II Automated Cell Counter (Cat. no. AMQAX1000)
1 each
Power Cord with 4 adaptor cords
(for U.S./Canada/Taiwan/Japan, Europe, or UK)
1 each
Countess™ Cell Counting Chamber Slides (50 slides/box)
1 box
Countess™ II Disposable Slide Holder
1 each
Countess™ II USB drive
1 each
Countess II Automated Cell Counter Quick Reference Card
1 each
™
™
Countess™ II
Automated Cell
Counter
Upon receiving the
instrument
The Countess™ II Automated Cell Counter is a fully automated cell counter and
assay platform that uses state-of-the-art optics and image analysis algorithms to
analyze trypan blue-stained cells in suspension.
•
The Countess™ II Automated Cell Counter offers an intuitive user interface,
and provides the option to save data and generate a report, which can then be
transferred to a PC using the USB drive supplied with the instrument or
available separately.
•
The cells to be counted are loaded into the instrument in disposable Countess™
Cell Counting Chamber Slides (page 13). Each chamber slide contains two
enclosed chambers to hold the sample to allow you to measure two different
samples or perform replicates of the same sample.
•
The Countess™ II Automated Cell Counter takes 10 seconds per sample for a
typical cell count and is compatible with a wide variety of eukaryotic cells. In
addition to cell count and viability, the instrument also provides information
on cell size.
Examine the instrument carefully for damage incurred during transit. Ensure that
all parts of the instrument, including accessories listed above, are included with the
product. Damage claims must be filed with the carrier; the warranty does not cover
in-transit damage.
See page 7 for instructions on installing the instrument.
Register your
instrument
Visit www.thermofisher.com/registercountess to register your instrument. You
will be asked to supply the serial number, your name, and your contact details.
Registering your instrument ensures that you will receive notifications of software
upgrades and information on new assays for use with the Countess™ II Automated
Cell Counter.
5
Instrument exterior components
Touch-screen display: The 7-inch capacitive touch-screen display is the main user interface of the
Countess™ II Automated Cell Counter. It contains the buttons for all instrument functions and displays
data from the cell count.
USB ports: The USB ports allow you to transfer and save the cell count data and image to an external
computer for record keeping and printing purposes. You can use the USB drive supplied with the
instrument or any other standard, FAT32-formatted USB drive for data transfer. If desired, you can plug
in a USB mouse into the rear USB port for instrument control.
Note: The USB ports located in the front and the back of the instrument function the same. However, the
first USB drive connected will be the preferred saving location and both USB drives cannot be accessed
at the same time.
Slide port: The slide port is used to insert the Countess™ Cell Counting Chamber Slide containing the
sample into the instrument.
Power switch: The ON/OFF rocker switch is the main power switch. It is not necessary to use the power
switch for day-to-day operation of the instrument.
Power input jack: The power input jack connects the instrument to an electrical outlet through the
supplied power cord and the appropriate plug, based on the electrical outlet configuration in your
country.
Note: The Countess™ II Automated Cell Counter (Cat. no. AMQAX1000) uses brightfield
illumination only and does not support the EVOS™ LED light cubes.
6
2. Getting started
Installation
Operating
environment
•
Place the instrument on a level surface away from vibrations emanating from
other pieces of equipment.
•
Allow at least 5 cm (2 in) free space at the back of the instrument to allow for
proper ventilation and prevent overheating of electronic components.
•
Set up the instrument away from direct light sources, such as windows.
Ambient room lighting can enter the imaging path and affect the image quality.
•
Operating temperature range: 4°–32°C (40°–90°F).
•
Relative humidity range: 30–90%.
IMPORTANT! Do not position the instrument so that it is difficult to turn off
the main power switch located on the back of the instrument (see page 6). In
case of an instrument malfunction, turn the main power switch to the OFF
position and disconnect the instrument from the wall outlet.
Install the
instrument
1. Unpack the instrument and place the instrument on a flat, level, dry surface.
2. Remove the thin plastic protector film from the touch-screen display.
3.
Plug one end of the power cord appropriate for your region into the instrument.
4. Plug the power cord into the electrical outlet. Be sure to use only the power
cord supplied with your instrument. Powering the instrument with an
unapproved power cord may damage the instrument.
7
Turn ON the
instrument
1. Turn on the instrument by flipping the power switch on the back of the
instrument (page 6) to the ON position.
The instrument initializes and displays the Home screen.
2. From the Home screen, you can proceed immediately to the assays by inserting
a slide (page 13).
Alternatively, you can change or add a profile (Step 3, below) or change
instrument settings (Step 4, below).
3. To change the current profile or to add a new profile to the instrument,
press the Profiles button in the upper left corner.
Profiles allow you to create customized count preferences (i.e., gate counts
based on cell size, brightness, and/or circularity) (page 9).
4. To change instruments settings, press the Instrument Settings button in
the upper right corner.
Instrument settings allow you to update the Countess™ II software and change
the date and time (page 23).
8
Load profile
Profiles screen
Profiles screen allows you to create and save up to 9 customized profiles. Each
custom profile defines the count parameters (size, brightness, and circularity) and
the Auto Lighting function for a consistent and streamlined workflow.
•
You can access the Profiles screen from the Home, Capture, Results, Advanced,
or Adjust screens.
•
The current profile is displayed on the upper left corner of
the Home, Capture, Results, Advanced, or Adjust screens.
•
The Auto Lighting function and count parameters are set in the Edit profile
screen (page 10).
•
The Default profile contains default count settings and cannot be edited.
•
The count parameters specified in the selected profile are applied to all new
cell counts.
•
If you have already performed a count, loading a new profile from the Results
screen applies the count preferences to the current counts results (total cells,
viability etc.) and to all new counts.
9
Edit profile screen
The Auto Lighting function and count parameters are set in the Edit profile screen.
Auto Lighting
Auto Lighting function automatically illuminates the sample in the brightfield
channel for increased sample-to-sample consistency and decreased user-to-user
variability. By default, the Auto Lighting function is turned on; to toggle it on and
off, use the Auto Lighting checkbox (see page 11).
Count parameters
Count parameters are set using the parameter sliders, which correspond to a single
channel that is selected via the channel selection radio buttons.
• Size: As you move the slider up, the algorithm includes larger objects in the
count. As you move the slider down, only the smaller objects are counted.
= larger objects
•
= smaller objects
Brightness: As you move the slider up, the algorithm includes the brighter
objects in the count. As you move the slider down, only the dimmest of objects
are counted.
= brighter objects
•
Circularity: As you move the slider up, the algorithm includes more objects
with shapes other than circular in the count. As you move the slider down,
only the objects that are perfect circles are counted.
= less circular
•
= dimmer objects
= more circular
Size and brightness sliders are range sliders.
To adjust the upper and lower boundaries without changing the data range,
drag the slider by its middle section (i.e., the slider bar).
To adjust only the upper or the lower boundary, move the upper or the lower
handle in the desired direction. This will also change the range of values
within which the cells are counted.
•
The circularity slider only sets a single threshold value; cells that fall below the
set value are counted, and cells that are beyond this range are excluded.
To adjust the threshold for circularity, drag the slider in the desired direction.
10
Load a profile
1. Press the Profiles button located on the upper left corner of the screen to
open the Profiles screen.
2. Press the desired profile to select, and then press Load.
The instrument will load the count parameters specified in the selected profile
and return to the previous screen.
3. To return to the previous screen without loading the new profile, press
the previous button.
The instrument will keep the saved profile, but return to the previous screen
without loading it.
Add/edit a profile
1. Press the Profiles button located on the upper left corner of the screen to
navigate to the Profiles screen.
2. To add or edit a new profile, select an empty or an existing profile, and then
press Edit. The Edit screen for the selected profile opens.
Note: The Default profile contains default count settings and cannot be edited.
3. Select or deselect the Auto Lighting checkbox to turn the Auto Lighting
function ON or OFF.
4. To define the new count parameters, select Live or Dead from the Trypan Blue
radio buttons.
5. Adjust the size, brightness, and circularity thresholds using the appropriate
parameter slider (page 10).
Note: Although the Auto FL Threshold checkbox and the FL channel
radio buttons are visible on the Edit profile screen, they are not
functional on Countess™ II Automated Cell Counters, which only
feature a single brightfield channel.
11
6. To assign a name to the new profile or to change the name of the existing
profile, press the Profile name text box. The alpha-numeric keypad opens.
7. Type in the desired profile name using the alpha-numeric keypad.
To enter symbols, press the symbol (@%&) key. To return to the alphanumeric keypad, press ABC.
8. Press Enter to save the name and return to the Edit profile screen.
To return to the Edit profile screen without saving the name, press the
close button.
9. Press Save to save the new profile, and then press Close in the confirmation
screen to return to the Profiles screen.
To return to the Profiles screen without saving, press Cancel.
10. On the Profiles screen, press Load. The instrument will load the count
parameters specified in the selected profile and return to the previous screen.
11. To return to the previous screen without loading the new profile, press
the previous button. The instrument will keep the saved profile, but
return to the previous screen without loading it.
12
3. Cell count and cell viability assays
Prepare sample
Recommendations
Load Countess™
Chamber Slide
To obtain the best results, follow these recommendations:
•
Ensure that the cell sample is homogeneously mixed.
•
The measurement range extends from 1 × 104–1 × 107 cells/mL, but the optimal
range is 1 × 105–4 × 106 cells/mL.
•
For accurate results in cell viability assays, ensure that the counting area is
covered with the cell suspension and count the cells immediately after staining
per the assay protocol.
•
Do not press the optical surfaces of the chamber slides. Hold the slides by the
edges.
•
Take care to avoid forming bubbles in the sample.
1. Prepare the sample by adding 10 μL of your cell suspension to 10 μL of 0.4%
trypan blue stain. Mix the sample mixture well by pipetting up and down a
few times.
2. Gently pipet 10 µL of the trypan blue-stained sample into the half moon-shaped
sample loading area. The sample is loaded into the chamber through capillary
action.
Note: Each chamber in the Countess™ Cell Counting Chamber Slide has a
10-µL sample capacity. Do not overfill the slide chambers.
3. Let the sample mixture settle in the chamber for 30 seconds, and then insert the
slide into the slide port (see page 6). You will hear a soft click, if the slide is
pushed in correctly.
4. To remove the slide, push the slide gently into the instrument until it "clicks"
and a spring pushes the slide out. Grasp the slide and pull it out the rest of the
way.
Note: After using the Countess™ Cell Counting Chamber Slides,
appropriately dispose of them as biohazardous waste. Do not reuse the
disposable chamber slides.
13
Count cells
Count procedure
1. Load the Countess™ Cell Counting Chamber Slide with your trypan bluestained sample and insert it into the slide port of the instrument as described
on page 13. You will hear a soft click, if the slide is pushed in correctly.
2.
When the slide is inserted, the instrument automatically illuminates the sample,
sets the intensity of brightfield illumination, and auto focuses on the cells.
Note: To turn off the Auto Lighting function, see page 10.
3. Optional: Press the Profiles button and load the desired profile
as described on page 11.
4. Optional: If desired, press the Focus button, and then use the Focus slider
to manually bring your sample into focus (see Note below).
If needed, Zoom in on the image while adjusting the focus.
Note: The auto focus algorithm is designed to highlight the differences
between live and dead cells. Therefore, the optimal focus level is where
the “live” cells have a light colored center and the “dead” cells are dark
throughout. To enable optimal auto focus functionality, you may need
to initially refine the focus by adjusting it manually and then setting it
as the nominal focus. For more information, see page 29.
5. Optional: Manually adjust exposure using the light source slider.
The light source slider controls the LED intensity, camera gain, and exposure
time and it is used for adjusting the image brightness.
14
6. Press Capture. The instrument temporarily captures the image and displays
the results (total concentration, percentage and concentration of live and dead
cells). For more information, see “Results”, page 16.
Next steps
•
To identify the objects (i.e., cells) counted as “live” or “dead”, press More to
navigate to the Advanced screen (page 17).
•
To see the distribution of live and dead cells in a graphical format, press the
Graph button (see page 18).
•
To gate the results by object size, brightness, or circularity, first press More to
open the Advanced screen, and then press Adjust to navigate to the Adjust
screen (page 19).
•
To permanently save the results, press Save (see page 20).
•
To perform a new count, remove the slide and reinsert it into the instrument to
count the sample in the second chamber.
15
4. Results
View results
Results screen
The Results screen for cell count and cell viability assays displays a composite
image of the objects counted and the results of the cell count and cell viability
calculations (total concentration, percentage and concentration of live and dead
cells).
Note: When performing brightfield (BF) cell counts and cell viability assays,
the counting algorithm assumes that you have diluted your cells 1:1 in
trypan blue and takes this dilution into account when calculating the total
cell concentration. The cell concentration displayed in the Results screen is
the original cell concentration before dilution into trypan blue.
16
Identify the cells counted
Advanced screen
The Advanced screen allows you to identify the objects (i.e., cells) counted in each
channel and included in the count results for further review. After reviewing the
marked objects, you can adjust the threshold for size, brightness, and/or circularity
as desired for your application.
Identify Live and
Dead cells
1. On the Results screen, click More. The Advanced screen opens.
2. To identify the cells that are included in the count as “live”, press the Live
button. “Live” cells will be circled in green on the screen.
To identify the cells that are included in the count as “dead”, press the Dead
button. “Dead” cells will be circled in red on the screen.
Note: You may select either or both options. In the example below, both Live
and Dead buttons are pressed and “live” and “dead” cells are marked with
green and red circles around them, respectively.
3. To unmark the cells identified as “live” (green) or “dead” (red) on the screen,
press the Live or the Dead button again, respectively.
17
Graph count results
View graph
For cell count and cell viability assays, you can view the distribution of cells (live
and/or dead) based on size in a graphical format.
Note: You can view the Graph on Results, Advanced, and Adjust screens.
1. To view the graph showing the distribution live and/or dead cells based
on cell size, press the Graph button.
2. To view the distribution of only the live or dead cells, check the corresponding
Live or Dead check box on the graph.
The graph will automatically update and display the distribution of cells based
on size only in the selected population.
3. Optional: Using the size, brightness, and circularity sliders, adjust the count
parameters. As you adjust the count parameters, the count results and the
graph will be automatically updated.
4. To close the graph, press the Graph button again.
18
Gate count results
Adjust screen
The Adjust screen contains the controls for gating count results based on size,
brightness, and circularity. You can adjust the count parameters before or after
performing a count.
Gate count results
1. On the Results screen, press More to open the Advanced screen.
2. Optional: Press the Live and/or the Dead button to identify the cells in the
selected population (see page 17).
3. On the Advanced screen, press Adjust to open the Adjust screen.
4. Optional: Press the Graph button to view the distribution of cells (live
and/or dead) based on size as you gate the count results (see page 18).
5. Select the channel (Live or Dead) you wish to gate.
6. Using the size, brightness, and circularity sliders, adjust the count parameters.
Note: For a description of the count parameters and count parameter controls
(i.e., parameter sliders), see page 10.
7. When finished, press Done to save the changes to count parameters and return
to the Advanced screen.
Press Cancel to return to the Results screen without saving the changes.
8. On the Advanced screen, press Count to recalculate your results with the new
count parameters.
9. To permanently save your results to an external computer using a USB flash
drive, see page 20.
19
5. Save results
Save count results
Save screen
The Countess™ II FL Automated Cell Counter allows you to save your data and
images using a USB flash drive. To save your experiment, you can choose from the
following options, in any combination:
•
Result: Saves the Results screen as it is displayed on the instrument, with or
without the Graph, in the selected image format (JPEG, BMP, PNG, or TIFF).
•
Images: Saves only the raw captured image in the selected image format
(JPEG, BMP, PNG, or TIFF).
•
Data: Saves the data from the experiment as a CSV file (comma separated
values). The CSV format allows for processing or re-displaying results with
any third party software or spreadsheet program. For more information on the
CSV file format, see “Appendix D: CSV file format”, page 35.
Note: If you wish to save your results with the Graph showing the
distribution of cells based on cell size or fluorescence intensity, make sure
that the desired graph is displayed on the Results screen.
Save procedure
1. To save your data, insert the Countess™ II USB drive (or equivalent) into an
available USB port on the instrument (see page 6).
Note: The USB ports located in the front and the back of the instrument
function the same. However, the first USB drive connected will be the
preferred saving location.
2. On the Results screen, press Save to navigate to the Save screen.
20
3. To assign a name to your experiment, press the
File name text field. The alpha-numeric keypad
opens.
4. Enter the file name using the alpha-numeric keypad.
To enter symbols, press the symbol (@%&) key. To return to the alphanumeric keypad, press ABC.
5. Press Enter to save the name and return to the Save screen.
To return to the Save screen without saving the name, press the close
button.
6. Select the desired mode(s) to save your experiment (Result, Images, Data).
You can select an individual mode (e.g., Result only) or any combination of
modes (e.g., Result, Images, and Data).
In the example below, Images and Data are selected.
21
7. By default, Result and Images are saved as JPEG files.
To choose a different file format, press the file type button.
Choose file type screen opens.
Note: Data can only be saved as a CSV file.
8. Press to select the desired file type. Available options are JPEG, BMP, PNG,
and TIFF.
After you make your selection, the instrument returns to the Save screen.
To return to the Save screen without changing the file format, press the
close button.
9. Press Save to save your experiment in the selected mode(s) in the USB drive.
10. Press Close and then transfer the USB drive to the desired location.
22
6. Instrument settings
Overview
Instrument Settings To access the Instrument Settings screen, press the Instrument Settings
button on the Home page (page 8).
screen
In the Instrument Settings screen, you can:
•
perform software update (page 24)
•
set the date and time (page 25)
Note: Although the Change Light Cube button is visible on the Instrument
Settings screen, this button is not functional on the Countess™ II Automated
Cell Counter, which is a brightfield-only instrument and does not support
EVOS™ light cubes.
23
Software update
Guidelines for
software update
•
The USB drive used for transferring the software update file must be FAT32
formatted; verify this before proceeding. If necessary, reformat the USB drive
to FAT32 following the recommended procedure for your operating system.
Note: Reformatting the USB drive will result in the loss of all files. Back up the
files in the USB drive prior to reformatting.
Update the
Countess™ II/II FL
software
•
The software update file must be saved on the top level of the USB drive, not
within a folder or a subfolder.
•
The software update file must be uncorrupted during transfer. Do not rename,
zip, or compress the software update file.
1. Go to www.thermofisher.com/countessupdate, and download the latest
Countess™ II/II FL software version to your desktop.
Note: The software update file has a version-specific name followed by the
extension .lft (e.g., Countess_II_v_1_0_202.lft for software version 1.0.202).
2. Copy the software update file onto the USB drive, making sure that it is saved
on the top level and not hidden within a folder.
3. Insert the USB drive into one of the USB ports of the instrument (see page 6).
4. Press the Instrument Settings button on the Home page (page 8) to open
the Instrument Settings screen (page 23).
5. Select Software Update from the Instrument Settings menu. The instrument
scans the USB drive for the latest software version.
6. When prompted, select Update Now.
7. Once the update has completed, restart the instrument.
24
Date/Time
Set the date and
time
1. Press the Instrument Settings button on the Home page (page 8) to open
the Instrument Settings screen.
2. Press Date/Time on the Instrument Settings menu to open the Date/Time screen.
3. Select the Date format you wish to use.
4. Press any Date text box (MM, DD, or YYYY) to open the Edit Date keypad.
5. Using the keypad, enter the date into Month, Day, and Year text boxes,
pressing Enter after each entry.
6. After you are finished entering the date, press the close button to return
to the Date/Time screen.
25
7. Select the Time format you wish to use. Available options are 12 Hour and
24 Hour formats.
8. Press any Time text box (Hours or Minutes) to open the Edit Time keypad.
9. Using the keypad, enter the time into Hours and Minutes text boxes, pressing
Enter after each entry.
10. After you are finished entering the time, press the close button to return
to the Date/Time screen.
11. If you have selected the 12 Hour format, select AM or PM.
12. Press Save to set the Time and Date and return to the Instrument Settings
screen.
Press Cancel to return to the Instrument Settings screen without saving your
changes.
26
7. Maintenance
Instrument care
General guidelines
for care
Power supply
•
Use the appropriate cleaning solutions for each component, as indicated in the
cleaning procedures on page 28.
•
If liquid spills on the instrument, turn off the power immediately and wipe dry.
Always use the correct power supply. The power adaptor specifications appear on
the serial number label (bottom of the instrument) and in the Technical
specifications section of this user guide (page 33). Damage due to an incompatible
power adaptor is not covered by warranty.
CAUTION! Never disassemble or service the instrument yourself. Do not
remove any covers or parts that require the use of a tool to obtain access to
moving parts. Operators must be trained before being allowed to perform
the hazardous operation. Unauthorized repairs may damage the
instrument or alter its functionality, which may void your warranty.
Contact your local distributor to arrange for service.
IMPORTANT! If you have any doubt about the compatibility of
decontamination or cleaning agents with parts of the equipment or with
material contained in it, contact Technical Support (page 46) or your local
distributor for information.
27
Clean the Countess™ II Automated Cell Counter
Introduction
We recommend cleaning the Countess™ II Automated Cell Counter periodically to
prevent the buildup of dust and dirt that might reduce its performance and cause
contamination.
CAUTION! To avoid electrical shock, always turn off the Countess™ II
Automated Cell Counter and unplug the power cord before cleaning or
decontaminating the instrument.
CAUTION! All biological samples and materials that come into contact with
them have the potential to transmit infectious diseases and are considered
biohazardous. Follow all applicable local, state/provincial, and/or national
regulations. Wear appropriate protective eyewear, clothing, and gloves.
IMPORTANT! Using a cleaning or decontaminating method other than that sp
manufacturer may result in damage to the instrument.
Clean the touchscreen
Clean the
instrument case
Decontaminate the
instrument
•
Wipe the touch-screen of the Countess™ II Automated Cell Counter using a soft,
lint-free cloth moistened with an LCD cleaning solution. Do not apply excessive
force during cleaning. Wipe the touch-screen dry immediately after cleaning.
•
Ensure that the cleaning solution does not enter the power button, the power
inlet, the slide port, or the USB ports.
•
Never pour or spray any liquids directly on the instrument to avoid electrical
shock when the instrument is plugged in.
•
Do not use abrasive cleaning solutions or material to prevent the touch-screen
from getting scratched.
•
Wipe the instrument case of the Countess™ II Automated Cell Counter using a
soft, lint-free cloth moistened with distilled water. Wipe the instrument dry
immediately after cleaning.
•
Ensure that water or other cleaning solutions do not enter the power button, the
power inlet, the slide port, or the USB ports.
•
Never pour or spray any liquids directly on the instrument to avoid electrical
shock when the instrument is plugged in.
•
Wipe the instrument case of the Countess™ II Automated Cell Counter using a
soft, lint-free cloth moistened with 70% alcohol. Wipe the instrument dry
immediately after cleaning.
•
Avoid using a bleach solution, because it may leave a residue of bleach crystals
on the instrument.
•
Ensure that water or other cleaning solutions do not enter the power button, the
power inlet, the slide port, or the USB ports.
•
Never pour or spray any liquids directly on the instrument to avoid electrical
shock when the instrument is plugged in.
28
Set nominal focus
Overview
Nominal focus is the Z-point (i.e., depth) around which the auto focus function
searches to provide fine focus to the sample.
The auto focus algorithm of the Countess™ II FL Automated Cell Counter is
designed to highlight the differences between live and dead cells in the brightfield
channel. The optimal focus level is where the “live” cells have a light colored center
and the “dead” cells are dark throughout (see examples below).
Focus is not optimal
Focus is optimal
To enable optimal auto focus functionality, you may need to initially refine the
brightfield focus by adjusting it manually and then setting the nominal focus. This
allows the auto focus function to have a set point from which to focus on the cells
in subsequent samples.
Set nominal focus
1. Prepare the sample by adding 10 μL of cell suspension to 10 μL of 0.4% trypan
blue stain. Mix the sample mixture well by pipetting up and down a few times.
2. Load 10 μL of the sample mixture into the Countess™ Cell Counting Chamber
Slide (page 13). Let the sample mixture settle for 30 seconds to ensure a uniform
focal plane.
3. Insert the sample slide into the slide port of the instrument, making sure that
the side containing the sample is inserted completely.
4. When the slide is inserted, the instrument automatically illuminates the sample,
sets the intensity of the brightfield light source, and auto focuses on the cells.
29
5. To manually adjust the focus, press the Focus button.
6. Use the Focus slider or the plus and minus buttons to refine the
brightfield focus.
Note: If needed, Zoom in on the image to adjust focus or lighting.
7. After finding the optimal focus, press Set to set the nominal focus.
Once the focus has been set, the Set button on the focus slider becomes
inactive, confirming that the focus setting has been stored.
30
Appendix A: Troubleshooting
Note: The software for the Countess™ II is updated regularly. If you are having any issues with
your experiments, first check the website to see if a new software version is available. You can
download the most recent version of the software from www.thermofisher.com/countessupdate.
You can also register your Countess™ II instrument at www.thermofisher.com/registercountess to
be informed of any future software updates.
Problem
Possible solutions
•
Autofocus does not seem to focus on
the cells very well
•
•
Some cells appear on the image but
are not included in the count
•
Adjust the size, brightness, and circularity gates for both live
and dead cells to include all of the cells in the count (page 18).
•
After including all of the cells in the count, you can narrow
the count criteria, if you wish to exclude cells of a certain size
or certain brightness.
When the gates are fully maximized, the CSV should indicate
0–60 for cell size and 0–255 for brightness.
•
Images are very bright and washed
out
Make sure there are no bubbles or debris visible on the screen
that could interfere with the autofocus and make it more
difficult to get the sample in the correct focal plane.
Ideally, the live cells should have bright centers compared to
the dead cells, which are dark throughout (page 14).
Setting the nominal focus will improve autofocus consistency
with future slides. To set the nominal focus, see page 29.
Enable Auto Lighting from the Profiles menu, or decrease the
bright field light intensity before counting the cells.
•
•
Getting incorrect concentration for the
Countess™ test beads
The beads can settle quickly in solution, which will affect the
concentration reading.
Vortex the bead stock on high for a full 30 seconds to resuspend,
and add 10 µL of the bead suspension to 10 µL of trypan blue
without delay.
•
Pipet the bead and trypan blue mixture up and down several
times to make sure it is well mixed, and immediately load
10 µL into the slide.
•
If you are pipetting different samples from the same cell
sample, the variability could be due to pipetting or mixing.
Use recently calibrated pipettors and make sure that the cells
are well suspended by pipetting up and down several times
before adding trypan blue.
•
Variable counts for the same sample of
cells
•
Pipet the bead and trypan blue mixture up and down several
times to make sure it is well mixed, and load 10 µL into the
slide without delay.
31
Problem
Possible solutions
•
If you are counting replicates of the exact same slide, visually
inspect that all cells are counted correctly in the image.
•
There may be a slightly different field of view each time a
slide is inserted. Depending on the concentration and
uniformity of the cells, this will cause some variability when
performing replicate counts of the same slide, although it
should be less than 10%.
•
When counting fewer cells, a small field of view change for
only a small number of cells can have a larger affect. Count
cells at a higher concentration to reduce variability.
•
Make sure that you do not shake or agitate the slide between
counts.
•
Ensure that the cells are focused correctly so that live cells
have bright centers and dead cells are dark throughout (see
page 14). If the cells are not well focused and look dark on the
screen, the Countess™ II will count them as dead cells.
•
If cells are well focused, have bright centers, and are being
counted as dead, confirm that they are within the appropriate
cell size range and try adjusting the settings.
If cells are exposed to trypan blue for a long period of time,
viability could be affected so slide should be prepared and
counted fresh each time for best results.
Gate out the debris using the size, brightness, and circularity
sliders.
Variable counts when performing
replicate counts of the same slide
Abnormally high percentage of dead
cells or live cells counted as dead
•
•
•
USB drive not recognized by the
instrument
•
Unable to update the Countess
software
The USB drive must be FAT32 formatted to be recognized by
the instrument. If it is not, reformat the USB drive to FAT32
(page 24).
Try another correctly formatted USB drive.
•
Make sure the USB drive is formatted to FAT32. If it is not,
reformat the USB drive to FAT32 before transferring the files
onto the USB drive for software update.
•
The update file must sit on the top level of the USB drive, not
within a folder or a subfolder.
•
•
File cannot be renamed in any way.
File cannot be zipped or compressed during distribution. It
must be uncorrupted during transfer and have a .lft suffix.
•
If needed, check that the USB port is functional by testing a
USB mouse.
™
32
Appendix B: Product specifications
Technical specifications
Physical
characteristics
Technical
specifications
Optics
Analysis slide
Instrument type:
Benchtop cell counter and suspension cell-based
assay platform
Instrument dimensions:
9 (W) × 5½ (D) × 9 inches (H)
Weight:
8 lbs
Operating power:
100–240 VAC, 0.58 A MAX
Frequency:
50/60 Hz
Electrical input:
12 VDC, 2 A
Installation site:
Indoor use only, Class A Environments
(i.e., non-residential or light industrial);
Pollution degree 2.
Operating temperature:
10–40°C
Operating humidity:
<80% (non-condensing)
Processing time:
~15 seconds
Sample concentration range:
1 × 104–1 × 107 cells/mL
Particle/cell diameter range:
4–60 µm (particles); 7–60 µm (cells)
Required sample volume:
10 µL
Firmware:
Countess™ Automated Cell Counting Platform
Software
USB Drive :
4 Gigabyte
Optics:
1 channel (brightfield)
Camera:
5 Mega pixels, 2.5× Optical Magnification
Material:
Poly(methyl methacrylate) (PMMA)
Dimensions:
25 mm (W) × 75 mm (D) × 1.7 mm (H)
Chamber volume:
10 µL
33
Appendix C: Ordering information
Countess™ II Automated Cell Counter
The Countess™ II instrument and instrument accessories are available from
Thermo Fisher Scientific. For more information, visit www.thermofisher.com or
contact Technical Support (page 46).
Product
Quantity
Cat. no.
Countess II Automated Cell Counter
1 each
AMQAX1000
Countess II power cord with four adapter
cords
1 each
AMEP4716
Countess™ II USB drive
1 each
A25751
Countess II Disposable Slide Holder
1 each
AMEP4745
™
™
™
Accessory products
The following products can be used with the Countess™ II Automated Cell
Counter and are available separately from Thermo Fisher Scientific. For more
information, visit www.thermofisher.com or contact Technical Support (page 46).
Product
Quantity
Cat. no.
Countess Cell Counting Chamber Slides,
50 Slides (100 counts)
1 box*
C10228
Countess™ Cell Counting Chamber Slides,
500 Slides (1000 Counts)
10 boxes*
C10312
Countess™ Cell Counting Chamber Slides,
1250 Slides (2500 Counts)
25 boxes*
C10313
Countess™ Cell Counting Chamber Slides,
2500 Slides (5000 Counts)
50 boxes*
C10314
Countess™ Cell Counting Chamber Slides,
5000 Slides (10,000 Counts)
100 boxes*
C10315
Countess™ Test Beads (1 × 106 beads/mL)
1 mL
C10284
2 × 1 mL
T10282
™
Trypan blue stain (0.4 %)
* Each box of Countess Cell Counting Chamber Slides contains 50 slides and
2 × 1 mL vials of trypan blue (0.4%), sufficient for 100 counts.
™
34
Appendix D: CSV file format
CSV file format, explained
Overview
Category
General
Trypan
Blue/Brightfield
General Details
Trypan
Blue/Brightfield
Count
Parameters
A comma-separated values (CSV) file stores tabular data (numbers and text) in plaintext form. Plain text means that the file is a sequence of characters, with no data that
has to be interpreted as binary numbers. A CSV file can be opened with any third
party software or spreadsheet program. The table below describes the categories of
the Countess™ II data saved as a CSV file and opened with a spreadsheet program.
Column
Name
Description
A
Number
Sequential sample run number
B
File Name
Name of file
C
Date & Time
Date and time of sample run
D
Mode
BF-Brightfield
E
Total Concentration
Concentration of the entire sample
F
Total cells counted
Total number of cells counted in the sample
G
Live concentration
Concentration of just the “live” portion of the sample
H
Live cells counted
Total number of “live” cells counted
I
Dead concentration
Concentration of just the “dead” portion of the sample
J
Dead cells counted
Total number of “dead” cells counted
K
Viability (%)
Percent viability of the sample based on trypan blue staining
L
Average size (um)
Average cell size in microns
X
Focus value
Focal position number
Y
BF Light intensity
Brightfield light intensity value from 0-100%
Z
Live Size min
Minimum size of “live” cells in microns
AA
Live Size max
Maximum size of “live” cells in microns
AB
Live Brightness min
“Live” adjustment slider value for minimum brightness
AC
Live Brightness max
“Live” adjustment slider value for maximum brightness
AD
Live Circularity
“Live” adjustment slider value for circularity
AE
Dead Size min
Minimum size of “dead” cells in microns
AF
Dead Size max
Maximum size of “dead” cells in microns
AG
Dead Bright min
“Dead” adjustment slider value for minimum brightness
AH
Dead Bright max
“Dead” adjustment slider value for maximum brightness
AI
Dead Circ
“Dead” adjustment slider value for circularity
35
Appendix E: Safety
Safety conventions used in this document
Safety alert words
Four safety alert words appear in this document at points where you need to be
aware of relevant hazards. Each alert word–IMPORTANT, CAUTION,
WARNING, DANGER–implies a particular level of observation or action:
IMPORTANT! Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not
avoided, may result in minor or moderate injury. It may also be used to
alert against unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Except for IMPORTANT! safety alerts, each safety alert word in the document
appears with an open triangle figure that contains a hazard symbol. These hazard
symbols are identical to the hazard icons that are affixed to the instrument (see
“Safety symbols”).
36
Symbols on instruments
Electrical symbols
The following table describes the electrical symbols that may be displayed.
Symbol
Description
Indicates the On position of the main power switch.
Indicates the Off position of the main power switch.
Indicates a standby switch by which the instrument is switched on to
the Standby condition. Hazardous voltage may be present if this
switch is on standby.
Indicates the On/Off position of a push-push main power switch.
Indicates a terminal that may be connected to the signal ground
reference of another instrument. This is not a protected ground
terminal.
Indicates a protective grounding terminal that must be connected to
earth ground before any other electrical connections are made to the
instrument.
Indicates a terminal that can receive or supply alternating current or
voltage.
Indicates a terminal that can receive or supply alternating or direct
current or voltage.
37
Safety symbols
The following table describes the safety symbols that may be displayed. Each
symbol may appear by itself or in combination with text that explains the relevant
hazard (see “Safety labels on instruments”). These safety symbols may also
appear next to DANGERS, WARNINGS, and CAUTIONS that occur in the text of
this and other product-support documents.
Symbol
Description
Indicates that you should consult the manual for further information
and to proceed with appropriate caution.
Indicates the presence of an electrical shock hazard and to proceed
with appropriate caution.
Indicates the presence of a hot surface or other high-temperature
hazard and to proceed with appropriate caution.
Indicates the presence of a laser inside the instrument and to proceed
with appropriate caution.
Indicates the presence of moving parts and to proceed with
appropriate caution.
Indicates the presence of a biological hazard and to proceed with
appropriate caution.
Indicates the presence of an ultraviolet light and to proceed with
appropriate caution.
Environmental
symbols
The following symbol applies to all Thermo Fisher Scientific electrical and
electronic products placed on the European market after August 13, 2005.
Symbol
Description
Do not dispose of this product as unsorted municipal waste. Follow
local municipal waste ordinances for proper disposal provisions to
reduce the environmental impact of waste electrical and electronic
equipment (WEEE).
European Union customers:
Call your Customer Service representative for equipment pick-up and
recycling. See www.thermofisher.com for a list of customer service
offices in the European Union.
38
Safety labels on instruments
The following CAUTION, WARNING, and DANGER statements may be
displayed Thermo Fisher Scientific instruments in combination with the safety
symbols described in the preceding section.
Hazard
symbol
English
Français
CAUTION! Hazardous chemicals. Read the
Safety Data Sheets (SDSs) before handling.
ATTENTION! Produits chimiques
dangereux. Lire les fiches techniques de
sûreté de matériels avant toute manipulation
de produits.
CAUTION! Hazardous waste. Refer to
SDS(s) and local regulations for handling and
disposal.
ATTENTION! Déchets dangereux. Lire les
fiches techniques de sûreté de matériels et la
régulation locale associées à la manipulation
et l’élimination des déchets.
DANGER! High voltage.
DANGER! Haute tension.
WARNING! To reduce the chance of
electrical shock, do not remove covers that
require tool access. No user-serviceable parts
are inside. Refer servicing to Thermo Fisher
Scientific qualified service personnel.
AVERTISSEMENT! Pour éviter les risques
d’électrocution, ne pas retirer les capots dont
l’ouverture nécessite l’utilisation d’outils.
L’instrument ne contient aucune pièce
réparable par l’utilisateur. Toute intervention
doit être effectuée par le personnel de service
qualifié venant de Thermo Fisher Scientific.
DANGER! Class 3B visible and/or invisible
laser radiation present when open. Avoid
exposure to beam.
DANGER! Rayonnement visible ou invisible
d’un faisceau laser de Classe 3B en cas
d’ouverture. Evitez toute exposition au
faisceau.
CAUTION! Moving parts. Crush/pinch
hazard.
ATTENTION! Pièces en mouvement, risque
de pincement et/ou d’écrasement.
39
General instrument safety
WARNING! PHYSICAL INJURY HAZARD. Use this product only as
specified in this document. Using this instrument in a manner not specified
by Thermo Fisher Scientific may result in personal injury or damage to the
instrument.
Operating the
instrument
Safety precautions
Ensure that anyone who operates the instrument has:
•
Received instructions in both general safety practices for laboratories and
specific safety practices for the instrument.
•
Read and understood all applicable Safety Data Sheets (SDSs). See
“Documentation and support” (page 46).
•
Do not install the instrument in heavy humidity such as a greenhouse or an
incubator to avoid a danger of electric shock. If water or other material enters
the instrument, the adaptor, or power inlet, disconnect the power cord and
contact a service person. For operating environment, refer to “Operating
environment” (page 7).
•
Do not press the main plug or power cord with wet hands.
•
Always ensure that the power supply input voltage matches the voltage
available in your location.
•
Do not install the instrument on a slant or a place prone to vibrations, which
induces the risk of instrument malfunction or damage of the instrument.
•
Never insert any objects into the air vents of the instrument as this could result
in electrical shock, personal injury, and equipment damage.
•
Plug the power cord firmly into the wall outlet and the instrument.
•
To avoid potential shock hazard, make sure that the power cord is properly
grounded.
•
Be sure to position the equipment such that it is easy to disconnect the
instrument.
•
Turn off the instrument before unplugging the power cord and/or moving the
instrument.
•
If the instrument is broken or dropped, disconnect the power cord and contact
a service person. Do not disassemble the instrument.
•
Use only authorized accessories (adaptor, power cord, and USB drive).
•
If the instrument emits smoke, disconnect the power cord from the wall outlet
and contact a service person.
Cleaning or
decontaminating the
instrument
CAUTION! Using cleaning or decontamination methods other than those
recommended by the manufacturer may compromise the safety or quality
of the instrument.
Removing covers or
parts of the
instrument
CAUTION! PHYSICAL INJURY HAZARD The instrument is to be
serviced only by trained personnel or vendor specified in the user guide.
40
Chemical safety
Chemical hazard
warning
WARNING! CHEMICAL HAZARD. Before handling any chemicals, refer
to the Safety Data Sheet (SDS) provided by the manufacturer, and observe
all relevant precautions.
WARNING! CHEMICAL STORAGE HAZARD. Never collect or store
waste in a glass container because of the risk of breaking or shattering.
Reagent and waste bottles can crack and leak. Each waste bottle should be
secured in a low-density polyethylene safety container with the cover
fastened and the handles locked in the upright position. Wear appropriate
eyewear, clothing, and gloves when handling reagent and waste bottles.
General safety
guidelines
To minimize the hazards of chemicals:
•
Read and understand the Safety Data Sheets (SDSs) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or
hazardous materials. See “Documentation and support” (page 46).
•
Minimize contact with chemicals. Wear appropriate personal protective
equipment when handling chemicals (for example, safety glasses, gloves, or
protective clothing). For additional safety guidelines, consult the SDS.
•
Minimize the inhalation of chemicals. Do not leave chemical containers open.
Use only with adequate ventilation (for example, fume hood). For additional
safety guidelines, consult the SDS.
•
Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturer’s cleanup procedures as recommended in the SDS.
•
Comply with all local, state/provincial, or national laws and regulations
related to chemical storage, handling, and disposal.
41
Chemical waste safety
Chemical waste
hazard
Chemical waste
safety guidelines
Waste disposal
CAUTION! HAZARDOUS WASTE. Refer to Safety Data Sheets (SDSs)
and local regulations for handling and disposal.
To minimize the hazards of chemical waste:
•
Read and understand the Safety Data Sheets (SDSs) provided by the
manufacturers of the chemicals in the waste container before you store,
handle, or dispose of chemical waste.
•
Provide primary and secondary waste containers. (A primary waste container
holds the immediate waste. A secondary container contains spills or leaks
from the primary container. Both containers must be compatible with the
waste material and meet federal, state, and local requirements for container
storage.)
•
Minimize contact with chemicals. Wear appropriate personal protective
equipment when handling chemicals (for example, safety glasses, gloves, or
protective clothing). For additional safety guidelines, consult the SDS.
•
Minimize the inhalation of chemicals. Do not leave chemical containers open.
Use only with adequate ventilation (for example, fume hood). For additional
safety guidelines, consult the SDS.
•
Handle chemical wastes in a fume hood.
•
After emptying the waste container, seal it with the cap provided.
•
Dispose of the contents of the waste tray and waste bottle in accordance with
good laboratory practices and local, state/provincial, or national
environmental and health regulations.
If potentially hazardous waste is generated when you operate the instrument, you
must:
•
Characterize (by analysis, if necessary) the waste generated by the particular
applications, reagents, and substrates used in your laboratory.
•
Ensure the health and safety of all personnel in your laboratory.
•
Ensure that the instrument waste is stored, transferred, transported, and
disposed of according to all local, state/provincial, and/or national
regulations.
IMPORTANT! Radioactive or biohazardous materials may require special
handling, and disposal limitations may apply.
42
Electrical safety
DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock can
result from operating the Countess™ II Automated Cell Counter without its
instrument panels in place. Do not remove instrument panels.
High‐voltage contacts are exposed when instrument panels are removed
from the instrument.
Fuses
WARNING! FIRE HAZARD. For continued protection against the risk of
fire, replace fuses only with fuses of the type and rating specified for the
instrument.
Power
DANGER! ELECTRICAL HAZARD. Grounding circuit continuity is vital
for the safe operation of equipment. Never operate equipment with the
grounding conductor disconnected.
DANGER! ELECTRICAL HAZARD. Use properly configured and
approved line cords for the voltage supply in your facility.
DANGER! ELECTRICAL HAZARD. Plug the system into a properly
grounded receptacle with adequate current capacity.
Overvoltage rating
The Countess™ II Automated Cell Counter has an installation (overvoltage)
category of II, and is classified as portable equipment.
43
Biological hazard safety
WARNING! BIOHAZARD. Biological samples such as tissues, body
fluids, and blood of humans and other animals have the potential to
transmit infectious diseases. Follow all applicable local, state/provincial,
and/or national regulations. Wear appropriate protective eyewear,
clothing, and gloves. Read and follow the guidelines in these publications.
ATTENTION! BIOHAZARD. Les échantillons biologiques tels que les
tissus, les fluides corporels et le sang des humains et d’autres animaux ont
la possibilité de transmettre des maladies infectieuses. Suivre tous les
règlements municipaux, provinciaux/provincial et / ou nationales en
vigueur. Porter des lunettes de protection approprié, des vêtements et des
gants.
In the U.S.:
•
U.S. Department of Health and Human Services guidelines published in
Biosafety in Microbiological and Biomedical Laboratories
(stock no. 017-040-00547-4;
www.cdc.gov/OD/ohs/biosfty/bmbl4/bmbl4toc.htm)
•
Occupational Safety and Health Standards, Bloodborne Pathogens
(29 CFR§1910.1030;
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html)
•
Your company’s/institution’s Biosafety Program protocols for working
with/handling potentially infectious materials.
•
Additional information about biohazard guidelines is available at:
www.cdc.gov
In the EU:
•
Check your local guidelines and legislation on biohazard and biosafety
precaution, and the best practices published in the World Health Organisation
(WHO) Laboratory Biosafety Manual, third edition
www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_
2004_11/en/
44
Safety and electromagnetic compatibility (EMC) standards
This section provides information on:
•
•
•
U.S. and Canadian
safety standards
U.S. and Canadian safety standards
European safety and EMC standards
Australian EMC standards
The CSA C/US Mark signifies that the product meets applicable U.S. and
Canadian standards, including those from CSA, CSA America, ANSI, ASME,
ASSE, ASTM, NSF and UL.
European safety and The CE Mark symbolizes that the product conforms to all applicable European
Community provisions for which this marking is required. Operation of the
EMC standards
instrument is subject to the conditions described in this manual.
The protection provided by the instrument may be impaired if the instrument is
used in a manner not specified by Thermo Fisher Scientific.
Australian EMC
standards
The C-Tick Mark indicates conformity with Australian and New Zealand
standards for electromagnetic compatibility.
45
Documentation and support
Obtaining support
Technical support
Visit www.thermofisher.com/support for the latest in services and support,
including:
•
Worldwide contact telephone numbers
•
Product support, including:
−
Product FAQs
−
Software, patches, and updates
•
Order and web support
•
Product documentation, including:
−
User guides, manuals, and protocols
−
Certificates of Analysis
−
Safety Data Sheets (SDSs; also known as MSDSs)
Note: For SDSs for reagents and chemicals from other manufacturers, contact the
manufacturer.
Limited product
warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set
forth in the Life Technologies’ General Terms and Conditions of Sale found on Life
Technologies’ website at www.lifetechnologies.com/termsandconditions.
If you have any questions, please contact Life Technologies at
www.lifetechnologies.com/support.
46
For support visit thermofisher.com/support
thermofisher.com
1 September 2015