Download Multidrop DW - User Manual

Thermo Scientific
Multidrop® DW
User Manual
Rev. 3.2
Thermo Scientific
Multidrop® DW
User Manual
Rev. 3.2
Table of Contents
Thermo Scientific Multidrop DW, Cat. no. 5840170 and 5840177
User Manual Rev. 3.2; November 2007, Cat. no. 1507060
Contents
1
Safety Symbols and Markings ....................................................................................................6
2
About the User Manual ...............................................................................................................7
3
Introduction to the Multidrop DW..............................................................................................8
4
Functional Description...............................................................................................................10
5
Installation ..................................................................................................................................15
6
Routine Operation......................................................................................................................20
7
Maintenance ...............................................................................................................................40
8
Technical Specifications ............................................................................................................64
9
Troubleshooting .........................................................................................................................70
10
Warranty Certificate ..................................................................................................................75
11
Ordering Information.................................................................................................................76
12
Glossary and Abbreviations......................................................................................................79
13
Index ...........................................................................................................................................81
14
Table of Figures..........................................................................................................................86
Appendix A. System Log......................................................................................................................88
Appendix B. Thermo Scientific Multidrop DW Brief User’s Guide ...................................................89
Appendix C. Thermo Scientific Multidrop DW Feedback Form........................................................90
Appendix D. Addresses ........................................................................................................................91
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
2
Table of Contents
Table of Contents
1
Safety Symbols and Markings .......................................................................................................6
2
About the User Manual...................................................................................................................7
3
Introduction to the Multidrop DW .................................................................................................8
3.1
3.2
3.3
4
INTENDED USE ..............................................................................................................................8
PRINCIPLE OF OPERATION...............................................................................................................8
ADVANTAGES OF USING THE MULTIDROP DW .................................................................................9
Functional Description ..................................................................................................................10
4.1
INSTRUMENT LAYOUT ..................................................................................................................10
4.1.1
Front view ........................................................................................................................10
4.1.2
Back view .........................................................................................................................13
4.2
MULTIDROP DW DEEP-WELL PLATE DISPENSER ..............................................................................14
5
Installation .....................................................................................................................................15
5.1
INSTALLATION CHECKLIST ............................................................................................................15
5.2
WHAT TO DO UPON DELIVERY.......................................................................................................15
5.2.1
How to unpack.................................................................................................................15
5.2.2
Checking delivery for completeness ..............................................................................15
5.2.3
Checking for damage during transport ..........................................................................16
5.2.4
Environmental requirements ..........................................................................................16
5.2.5
Things to avoid ................................................................................................................16
5.2.6
Technical prerequisites ...................................................................................................16
5.3
HOW TO ENSURE STARTUP...........................................................................................................17
5.4
OPERATIONAL CHECK ..................................................................................................................18
5.5
OPERATING PRECAUTIONS AND LIMITATIONS BEFORE OPERATION ....................................................19
6
Routine Operation .........................................................................................................................20
6.1
OPERATIONAL CHECKLIST ............................................................................................................20
6.2
SWITCHING ON ...........................................................................................................................20
6.3
HOW TO INSERT THE DISPENSING CASSETTE AND THE PRIMING VESSEL ............................................21
6.4
HOW TO PLACE DIFFERENT TYPES OF PLATES .................................................................................24
6.4.1
Without plate adapters ....................................................................................................24
6.4.2
With plate adapters .........................................................................................................25
6.5
HOW TO USE THE CONTROL PANEL ...............................................................................................30
6.5.1
Control panel indicators ..................................................................................................30
6.5.2
Control panel thumbwheels (digital switches)...............................................................30
6.5.3
Control panel keys ...........................................................................................................31
6.6
HOW TO PRIME ...........................................................................................................................31
6.7
HOW TO CHECK AND ADJUST THE PUMP ROTOR HEIGHT FOR 50 MM HIGH PLATES .............................32
6.8
DISPENSING ...............................................................................................................................36
6.8.1
How to dispense automatically.......................................................................................36
6.8.2
How to dispense manually into the specified column(s) ..............................................36
6.8.3
How to dispense multiple volumes ................................................................................37
6.8.3.1
Dispensing tenfold and hundredfold volumes......................................................................... 37
6.8.4
After use...........................................................................................................................38
6.9
SHUTDOWN ................................................................................................................................39
6.10
GUIDELINES FOR USE ...............................................................................................................39
7
Maintenance ..................................................................................................................................40
7.1
REGULAR AND PREVENTIVE MAINTENANCE ....................................................................................40
7.1.1
General .............................................................................................................................40
7.1.2
Immediate ........................................................................................................................40
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
3
Table of Contents
7.2
HOW TO WASH THE DISPENSING CASSETTE ...................................................................................41
7.3
HOW TO AUTOCLAVE THE DISPENSING CASSETTE ...........................................................................41
7.4
HOW TO REPLACE THE FUSES .......................................................................................................42
7.5
HOW TO RECALIBRATE THE DISPENSING CASSETTE .........................................................................44
7.5.1
Volumetric calibration .....................................................................................................44
7.5.2
Gravimetric calibration ....................................................................................................46
7.6
HOW TO CHANGE THE TUBING SET ................................................................................................49
7.6.1
Changing the tip band .....................................................................................................49
7.6.2
Fitting the new tubing set step by step ..........................................................................50
7.7
DISPOSAL OF MATERIALS .............................................................................................................61
7.8
DECONTAMINATION PROCEDURE ..................................................................................................61
7.9
MAINTAINING A SYSTEM LOG .......................................................................................................62
7.10
HOW TO PACK FOR SERVICE ......................................................................................................63
7.11
SERVICE CONTRACTS ...............................................................................................................63
7.12
DISPOSAL OF THE INSTRUMENT ................................................................................................63
8
Technical Specifications ...............................................................................................................64
8.1
GENERAL SPECIFICATIONS ...........................................................................................................64
8.2
SAFETY SPECIFICATIONS ..............................................................................................................65
8.2.1
Live parts..........................................................................................................................65
8.3
IN CONFORMITY WITH THE REQUIREMENTS ....................................................................................66
8.4
REMOTE CONTROL TO MULTIDROP DW ........................................................................................67
8.4.1
Hardware for the computer port.....................................................................................67
8.4.2
Multidrop DW PC interface .............................................................................................67
9
Troubleshooting ............................................................................................................................70
9.1
TROUBLESHOOTING GUIDE ...........................................................................................................70
9.2
FREQUENTLY ASKED QUESTIONS (FAQ) ABOUT THE MULTIDROP DW .............................................71
9.3
HAZARDS ...................................................................................................................................72
9.3.1
Electrical...........................................................................................................................72
9.3.2
Mechanical .......................................................................................................................72
9.3.3
Environmental..................................................................................................................72
9.3.4
Defects and abnormal stresses.......................................................................................73
9.4
SERVICE REQUEST PROTOCOL ......................................................................................................73
9.5
CERTIFICATE OF DECONTAMINATION.............................................................................................74
10
10.1
11
11.1
11.2
11.3
11.4
11.5
11.6
11.7
11.8
12
12.1
12.2
Warranty Certificate ..................................................................................................................75
WARRANTY LIMITATIONS .........................................................................................................75
Ordering Information.................................................................................................................76
INSTRUMENTS .........................................................................................................................76
DISPENSING CASSETTES ..........................................................................................................76
SPARE PARTS FOR DISPENSING CASSETTES ................................................................................77
CONSUMABLES .......................................................................................................................77
LIST OF ACCESSORIES ..............................................................................................................77
LIST OF PLATES (CONSUMABLES) ..............................................................................................77
LIST OF SPARE PARTS ..............................................................................................................78
LIST OF RECOMMENDED SPARE PARTS .......................................................................................78
Glossary and Abbreviations......................................................................................................79
KEYWORDS FOR WEB PAGES.....................................................................................................80
LITERATURE ............................................................................................................................80
13
Index ...........................................................................................................................................81
14
Table of Figures..........................................................................................................................86
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
4
Table of Contents
Appendix A. System Log......................................................................................................................88
Appendix B. Thermo Scientific Multidrop DW Brief User’s Guide ...................................................89
Appendix C. Thermo Scientific Multidrop DW Feedback Form........................................................90
Appendix D. Addresses ........................................................................................................................91
Copyright
Copyright © 2007 Thermo Fisher Scientific. All rights reserved. First edition published in 1997.
Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is
prohibited.
Patents
This product is protected by the following patent: US 4995432.
Trademarks (® and ™)
Immulon, Microfluor, Microtiter and Multidrop are registered trademarks of Thermo Fisher
Scientific.
Microlite is a trademark of Thermo Fisher Scientific.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to
incorporate technological developments. This manual is subject to change without prior notice as
part of a continuous product development.
Although this manual has been prepared with every precaution to ensure accuracy, Thermo
Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting
from the application or use of this information. This manual supersedes all previous editions.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or
inability to use this product.
Contact information
Thermo Fisher Scientific Oy
P.O. Box 100, FI-01621 Vantaa, Finland
Tel. +358-9-329 100, Fax +358-9-3291 0415
www.thermo.com
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
5
Chapter 1: Safety Symbols and Markings
1 Safety Symbols and Markings
These symbols are intended to draw your attention to essential information and alert you to the
presence of hazards as indicated.
SAFETY SYMBOLS USED ON THE MULTIDROP DW
Power ON
Power OFF
WEEE symbol
This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2002/96/EC.
WARNING MARKINGS USED IN THE DOCUMENTATION
Caution: risk of electric shock.
Caution: biohazard risk.
Caution: risk of personal injury to the operator or a safety hazard to the
surrounding area.
Caution: risk of damage to the instrument, other equipment or loss of
performance or function in a specific application.
)
Helpful hints, additional information.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
6
Chapter 2: About the User Manual
2 About the User Manual
This user manual has been written for the actual end user (for example, laboratory technician) and
provides information on the Thermo Scientific Multidrop DW deep-well plate dispenser, including
installation and operating instructions.
Read the manual in its entirety prior to operating the instrument.
This user manual has been designed to give you the information you need to:
•
•
•
•
•
•
Review safety precautions
Install the Multidrop DW
Carry out dispensing procedures
Recalibrate the dispensing cassette
Perform basic maintenance procedures
Troubleshoot the instrument performance.
This user manual also describes features and specifications of the Multidrop DW hardware and
onboard software.
Chapter 6 Routine Operation explains the dispensing principles and procedures.
In Chapter 9 Troubleshooting you will find a problem-solving guide. The user should be familiar
with the contents of Chapter 7 on maintenance.
For warranty and ordering information, refer to Chapters 10 Warranty Certificate and 11 Ordering
Information.
In an effort to produce useful and appropriate documentation, we appreciate your comments on
this document to your local Thermo Fisher Scientific representative.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
7
Chapter 3: Introduction to the Multidrop DW
3 Introduction to the Multidrop DW
There are two models of the Multidrop DW:
•
5840170
Multidrop DW, 220 ― 240 V 50/60 Hz
•
5840177
Multidrop DW, 100 ― 120 V 50/60 Hz
Each Multidrop DW comes with one dispensing cassette:
•
24072670 Dispensing cassette, extruded covers (with 40 cm tubing set)
3.1 Intended use
The Multidrop DW deep-well plate dispenser (Fig. 3.1) is intended for professional research use by
trained personnel. The instrument is intended for automated dispensing of up to eight different
reagents simultaneously into deep-well plates in 96-well format, tubes in a 96-channel rack and
96-well plates. Use for self-testing is excluded.
3.2 Principle of operation
The Multidrop DW is an automatic, programmable, eight-channel deep-well plate dispenser. It has
a peristaltic pump for rapid and continuous dispensing of liquids into deep-well plates, tubes in a
96-channel rack or 96-well plates. It can dispense one to eight different reagents from external
liquid reservoirs into different rows and can be used, for example, in the fields of drug discovery,
bacteriology, cell biology, hematology, immunology, toxicology and virology. It is particularly
useful in the pharmaceutical industry. The Multidrop DW is lightweight, robot compatible and
compact on a laboratory bench. It can dispense 20 µl into the entire 96-well microplate in
5 seconds or 900 µl into the entire deep-well plate in 74 seconds.
Fig. 3.1 Multidrop DW deep-well plate dispenser
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
8
Chapter 3: Introduction to the Multidrop DW
3.3 Advantages of using the Multidrop DW
The Multidrop DW deep-well plate dispenser provides several advantages relating mainly to the
principle of operation in that it has:
•
•
•
•
•
Dispensing for repetitive dispensing of large volumes
Superior accuracy and precision
A flexible volume range
High-speed continuous dispensing
An autoclavable, detachable and calibrateable dispensing cassette
The Multidrop DW is an automated deep-well plate dispenser designed for high-throughput
screening and microvolume dispensing into deep-well plates, tubes in a 96-channel rack or
standard 96-well plates.
The Multidrop DW can dispense up to eight different reagents with high accuracy, precision and
speed. It fills 20 µl into the entire 96-well microplate in 5 seconds or 900 µl into the entire deepwell plate in 74 seconds. With a volume range of 20 to 995 µl for deep-well plates, tubes in a
96-channel rack or 96-well plates, the Multidrop DW offers superior flexibility for a wide range of
applications.
All reagent lines can be backflushed to the reagent bottle, minimizing the loss of expensive
reagents. From 1 to 12 columns can be selected for deep-well plates, tubes in a 96-channel rack or
96-well plates.
To ensure sterility and avoid cross-contamination, the dispensing cassette is detachable and
autoclavable.
The Multidrop DW can be used as a standalone instrument or controlled with a PC via an RS-232
serial port.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
9
Chapter 4: Functional Description
4 Functional Description
4.1 Instrument layout
4.1.1 Front view
1
3
12
9
8
7
10
11
2
4
13
1
2
3
4
5
6
7
8
9
10
11
12
13
14
5
6
Instrument cover
Control panel
Thumbwheels for selecting the volume (volume
Plate carrier
Transfer rails
Priming vessel
Lower part slots
Upper part slots
Rest position slots of the upper part
Pump rotor
Rotor needles
Rotor cover not pulled over the rotor
Pump body
Plate adapter
14
µl) and number of columns (col)
Fig. 4.1 Multidrop DW front view (A)
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
10
Chapter 4: Functional Description
2
1
2
3
1
2
Mains switch (ON/OFF)
Instrument cover retaining screws
Cooling-air outlet
3
Fig. 4.2 Multidrop DW front view (B)
2
1
3
5
6
1
2
3
4
5
6
4
Rotor cover pulled over the rotor
Dispensing cassette attached
Deep-well plate
Plate carrier
Adjustment screws
Plate adapter lock
Fig. 4.3 Multidrop DW front view (C)
To adjust the plate size, loosen first the two attached plastic-covered screws on the right of the
plate carrier (Fig. 4.3). Push the microplate at the same time towards the left-hand top corner of
the plate adapter while you fasten the front screws.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
11
Chapter 4: Functional Description
3
2
4
1
5
8
6
7
1
2
3
4
5
6
7
8
Tips (8)
Lower part of dispensing cassette with tips
Tension limiting wires (2)
Upper part of dispensing cassette with tubes
Sight strips (8)
Tubes (A ― H)
Tubing weight, eight tubes
Calibration screw cover
Fig. 4.4 Close-up of the Multidrop DW dispensing cassette, extruded covers
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
12
Chapter 4: Functional Description
4.1.2 Back view
1
3
1
2
3
2
Mains input socket
Fuses in fuse holder cap
RS-232C serial interface connector
Fig. 4.5 Multidrop DW rear view
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
13
Chapter 4: Functional Description
4.2 Multidrop DW deep-well plate dispenser
The Multidrop DW deep-well plate dispenser (Fig. 4.6) automates dispensing into deep-well
plates. The microprocessor-based system permits volumes between 20 to 995 µl for deep-well
plates, tubes in a 96-channel rack or 96-well plates to be selected (in 5 µl increments) and then
dispensed simultaneously into eight wells (one column of a deep-well plate, tubes in a 96-channel
rack or a 96-well plate). Setting the volume to be dispensed and the number of columns per plate
(maximum 12 columns in deep-well plates, tubes in a 96-channel rack or 96-well plates) is carried
out using thumbwheels.
The movement of the microplate is automatic:
The columns with eight wells are filled starting from column 1 up to the column number set, after
which the plate carrier will return to the home position.
Normally, the deep-well plate moves through the instrument automatically, but a semiautomatic
mode is possible by using the step and drop keys, which control dispensing into any column of
the plate.
The dispensing cassette contains eight individual tubes. The dispensing cassette is detachable.
Each reagent can have a separate dispensing cassette. Alternatively, the same dispensing cassette
can be used with several reagents using the backflush feature. Refer to Section 11.2 Dispensing
cassettes. All dispensing cassettes can be autoclaved and Thermo Fisher Scientific guarantees
them up to 50 autoclavings. After this amount we recommend you replace the cassette.
To avoid damaging the tubes, follow the dispensing cassette maintenance, washing, autoclaving
and storage instructions carefully (see the instructions in Sections 6.8.4 After use, 7.2 How to
wash the dispensing cassette and 7.3 How to autoclave the dispensing cassette).
Fig. 4.6 Multidrop DW deep-well plate dispenser
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
14
Chapter 5: Installation
5 Installation
5.1 Installation checklist
The chapter on installation will contain an outline of the points mentioned in the checklist below.
Tick
Item
T
Unpack the Multidrop DW instrument carefully. Refer to 5.2.1. Keep the original packaging and
packing material for future transportation.
T
Check the delivery for completeness. Refer to 5.2.2.
T
Check for damage during transport. Refer to 5.2.3.
T
Place the dispenser on a normal laboratory bench taking into account both the environmental and
technical prerequisites. Refer to 5.2.4 and 5.2.6. Leave sufficient clearance on both sides and at the
rear of the unit.
T
Install the instrument.
T
Connect the mains supply cable to the mains input socket. Refer to 5.3.
T
Carry out the operational check. Refer to 5.4.
5.2 What to do upon delivery
5.2.1 How to unpack
Move the unpacked instrument to its site of operation. Unpack the Multidrop DW instrument and
accessories carefully with the arrows on the transport package pointing upwards. The following
notes and instructions are sent with the instrument and are immediately available when you open
the package:
•
•
•
Warranty Certificate card
packing instructions/packing list
Multidrop DW User Manual
Caution: Do not touch or loosen any screws or parts other than those specially
designated in the instructions. Doing so might cause misalignment and will invalidate
the instrument warranty.
Retain the original packaging and packing material for future transportation. The packaging is
designed to assure safe transport and minimize transit damage. Use of alternative packaging
materials may invalidate the warranty. Also retain all instrument-related documentation provided
by the manufacturer for future use.
5.2.2 Checking delivery for completeness
Check the enclosed packing list against order. In case of any deviations, contact your local
Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
15
Chapter 5: Installation
5.2.3 Checking for damage during transport
Visually inspect the transport package, the instrument and the accessories for any possible
transport damage.
If the carton has been damaged in transit, it is particularly important that you retain it for
inspection by the carrier in case there has also been damage to the instrument.
Neither the manufacturer nor its agents can be held responsible for any damage incurred in
transit, but the manufacturer will make every effort to help obtain restitution from the carrier.
Upon receipt of the carrier's inspection report, arrangements will be made for repair or
replacement.
Visually check all interconnections in the basic instrument. Check that there are no loose parts
inside the instrument.
If any parts are damaged, contact your local Thermo Fisher Scientific representative or Thermo
Fisher Scientific Oy.
5.2.4 Environmental requirements
When you set up your Multidrop DW, avoid sites of operation with excess dust, vibrations, strong
magnetic fields, direct sunlight, draft, excessive moisture or large temperature fluctuations.
•
•
•
•
Make sure the working area is flat, dry, clean and vibration-proof and leave additional room
for accessories, cables, reagent bottles, etc.
Make sure the ambient air is clean and free of corrosive vapors, smoke and dust.
Make sure the ambient temperature range is between +10°C (50°F) and +40°C (104°F).
Make sure relative humidity is between 10% and 80% (non-condensing).
Leave sufficient space (at least 10 cm) on both sides and at the back of the unit to allow adequate
air circulation.
The Multidrop DW does not produce operating noise at a level that would be harmful. No sound
level measurements are required after installation.
Warning: Do not operate the instrument in an environment where potentially
damaging liquids or gases are present.
5.2.5 Things to avoid
Do not smoke, eat or drink while using the Multidrop DW. Wash your hands thoroughly after
handling test fluids. Observe normal laboratory procedures for handling potentially dangerous
samples. Use proper protective clothing. Use disposable gloves. Be sure the working area is well
ventilated.
Never spill fluids in or on the equipment.
5.2.6 Technical prerequisites
Place the instrument on a normal laboratory bench. The net weight of the entire equipment is
approx. 6.2 kg [13.7 lbs.] and the dispensing cassette 124 g [0.3 lb.].
The instrument operates at voltages of 100 – 120 Vac and 220 – 240 Vac and the frequency range
50/60 Hz.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
16
Chapter 5: Installation
5.3 How to ensure startup
1. Warning: Ensure that the mains switch (Fig. 4.2) on the rear of the left side panel is
in the OFF position.
2. Connect the mains supply cable to the mains input socket (Fig. 4.3 and
Fig. 5.1) at the bottom of the back panel. If you need to use any other type of mains
supply cable than supplied, use only cables certified by the local authorities.
3. Connect the instrument to a correctly installed line power outlet that has a
protective conductor that is grounded.
Warning: Never operate your instrument from a power outlet that has no ground
connection. Never use a mains supply cable other than the Thermo Scientific
mains supply cable designed for your region.
Fig. 5.1 Connecting the mains supply cable
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
17
Chapter 5: Installation
5.4 Operational check
The following procedure should be completed without the dispensing cassette to confirm the
correct functioning of the dispenser prior to normal use.
1
Connect the mains supply cable (Fig. 5.1).
Switch the instrument on using the mains switch (Fig. 4.2).
•
If the instrument starts:
-
•
2
4
5
If the instrument does not start:
)Pull the rotor cover over the rotor
)Check the mains cable connection
Press prime/drop to prime
The pump rotor will rotate
•
If the pump rotor does not rotate:
)Pull the rotor cover over the rotor
•
3
The green power LED will light up
The plate carrier will return to the home position
If the red error LED lights up:
)Insert the priming vessel, press the stop key and prime again
Press start
Starts the dispensing cycle
•
If the dispensing cycle does not start:
)Pull the rotor cover over the rotor
•
If the red error LED lights up:
•
Advances the microplate by one column
If the microplate does not advance:
)Pull the rotor cover over the rotor
step
)Press the stop key, then press the prime key and finally press the start key again
)Insert the priming vessel, press the stop key, then press the prime key and finally press
the start key again
After step press prime/drop
To dispense a preselected volume into the column selected by steps
The plate carrier will return to the home position after reaching the last column
After step press start (partial filling of the tubing)
To dispense the selected volume automatically from the set column to the last column
6
7
empty
•
The pump rotor will rotate
If the pump rotor does not rotate:
)Pull the rotor cover over the rotor
-
Terminates the operation and returns the plate to the home position
stop
For more information on the control panel, refer to Section 6.5 How to use the control panel.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
18
Chapter 5: Installation
5.5 Operating precautions and limitations before operation
1.
Read this manual in its entirety, as it contains information necessary to ensure safe operation.
2.
Always ensure that the local supply voltage in the laboratory conforms to that specified on the
type label on the back of the instrument (Fig. 5.1).
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
19
Chapter 6: Routine Operation
6 Routine Operation
6.1 Operational checklist
The chapter on routine operation will contain an outline of the points mentioned in the checklist
below.
Tick
Item
T
Switch on the instrument. Refer to 6.2.
T
Insert the dispensing cassette and the priming vessel. Refer to 6.3.
T
Use the plate adapter if needed. Refer to 6.4.
T
Pull the rotor cover over the rotor. Refer to 6.3.
T
Familiarize yourself with the control panel. Refer to 6.5.
T
Prime all the eight channels so that there are no more air bubbles in the tubes. Refer to 6.6.
T
Adjust the rotor height for 50 mm high plates. Refer to 6.7.
T
Carry out dispensing either manually or automatically. Refer to 6.8.
T
After all the deep-well plates have been dispensed, press the
liquid from the tubes to the reservoir. Refer to 6.8.4.
T
Wash the dispensing cassette by priming it with deionized distilled water when not in use. Refer to
6.8.4 and 7.2.
T
After washing, store the dispensing cassette in the rest position. Refer to 6.8.4 and 7.2.
T
Maintain the instrument regularly. Refer to 7.
empty key to return the reagent or
Note: Keep the dispensing cassette in the rest position (Fig. 6.24) when the dispensing
cassette is not in use.
6.2 Switching on
Switch on the instrument by using the mains switch on the left-hand side of the instrument
(Fig. 4.2).
The green power LED will light up.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
20
Chapter 6: Routine Operation
6.3 How to insert the dispensing cassette and the priming vessel
1.
Take the lower part of the cassette into your right hand with the dispensing tips pointing
down and the upper part into your left hand (Fig. 6.1).
Fig. 6.1 Inserting the dispensing cassette
2.
Carefully place the eight tubes below the pump rotor and insert the lower part of the cassette
into the lower part slots of the pump body (Fig. 6.2 and Fig. 4.1, item 13). Check that the
tubes are freely placed below the rotor and the tension limiting wires (Fig. 4.4, item 3) below
the rotor shaft.
Lower part slots
Fig. 6.2 Inserting the lower part of the dispensing cassette into its slots
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
21
Chapter 6: Routine Operation
3.
Take a firm grip on the upper part of the cassette with your right hand and carefully pull the
tubes around the pump rotor until the upper part reaches and fits into the upper part slots of
the pump body (Fig. 6.3 and Fig. 4.1).
Fig. 6.3 Inserting the upper part of the dispensing cassette
Note: The tension limiting wires are designed to prevent the user from pulling the
tubes too much as excessive tension may damage the tubes or change the calibration
of the cassette.
4.
Double-check that both the upper and the lower parts are properly placed into their
corresponding slots (Fig. 6.2 and Fig. 6.4).
Upper part slots
Fig. 6.4 Lower and upper parts of the dispensing cassette inserted evenly into their slots
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
22
Chapter 6: Routine Operation
5.
Ensure that all the tubes are evenly placed on the rotor needles (Fig. 4.1, item 11), four tubes
on each half of the rotor.
6.
Also ensure that the tension limiting wires have a loose fit around the rotor shaft.
7.
Ensure that the priming vessel is inserted correctly into its slot on the left of the plate carrier.
You have fastened it correctly when it snaps slightly and is inserted into the sensor slot
(Fig. 6.5).
Priming vessel
sensor slot
Fig. 6.5 Inserting the priming vessel into place
8.
PULL THE ROTOR COVER OVER THE ROTOR (Fig. 6.6). The plate carrier will then return to
the furthest right of the instrument, that is, the home position. The microswitch controls that
the cover is properly placed over the rotor. The Multidrop DW is now ready for priming.
Fig. 6.6 Pulling the rotor cover over the rotor
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
23
Chapter 6: Routine Operation
Note: If the rotor cover is not pulled over the rotor, the instrument will not function.
Note: We recommend you change the dispensing cassette or tubing set after
dispensing about 3000 to 5000 96-well plates depending on the dispensing frequency
and the liquids used.
Note: Keep the dispensing cassette in the rest position (Fig. 6.24) when the dispensing
cassette is not in use.
6.4 How to place different types of plates
6.4.1 Without plate adapters
The Multidrop DW does not require a plate adapter for a) deep-well plates or b) Micronic tube
racks.
The deep-well plate is placed onto the plate carrier in the following way:
Α
Place the deep-well plate straight onto the plate carrier with the A1 corner of the plate located
top left of the plate carrier (Fig. 6.7).
A1 corner
Fig. 6.7 Deep-well plate placed onto the plate carrier
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
24
Chapter 6: Routine Operation
The Micronic tube rack is placed onto the plate carrier in the following way:
Β
Place the Micronic tube rack straight onto the plate carrier with the A1 corner of the rack
located top left of the plate carrier (Fig. 6.8).
A1 corner
Fig. 6.8 Micronic tube rack placed onto the plate carrier
6.4.2 With plate adapters
The Multidrop DW requires a plate adapter for the plate carrier to function when you use a) 96well plates or b) tubes in a 96-channel rack. Two different plate adapters are included (Fig. 6.9).
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
25
Chapter 6: Routine Operation
This plate adapter is suitable for 96-well plates
(Plate adapter for normal plate, Cat. no. 21040620).
This plate adapter is suitable for tubes in a 96-channel
rack (Plate adapter for 1.1 ml Thermo Scientific tubes,
Cat. no. 10260260).
Fig. 6.9 Two different plate adapters
A
The plate adapter for the 96-well plate is inserted into the plate carrier in the following way:
•
First place the plate adapter for the 96-well plate into the plate carrier (Fig. 6.10).
Fig. 6.10 Plate adapter for the 96-well plate inserted into the plate carrier
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
26
Chapter 6: Routine Operation
•
Then place the 96-well plate onto the plate adapter with the A1 corner of the plate located top
left of the plate carrier (Fig. 6.11).
A1 corner
Fig. 6.11 96-well plate placed onto the plate adapter
Note: Ensure that the dispensing cassette is attached and the rotor cover has been
pulled over the rotor.
B
The plate adapter for tubes in a 96-channel rack is inserted into the plate carrier in the
following way:
•
First place the plate adapter for the tubes in a 96-channel rack firmly into the plate carrier
(Fig. 6.12).
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
27
Chapter 6: Routine Operation
Fig. 6.12 Plate adapter for tubes in a 96-channel rack inserted into the plate carrier
•
Then place the tubes in the 96-channel rack onto the plate adapter with the A1 corner of the
rack located top left of the plate carrier (Fig. 6.13).
A1 corner
Fig. 6.13 Tubes in the 96-channel rack placed onto the plate adapter
Note: Ensure that the dispensing cassette is attached and the rotor cover has been
pulled over the rotor.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
28
Chapter 6: Routine Operation
•
The 1.1 ml tubes in the 96-channel rack (Cat. no. 9520770) are covered with 8-channel covers
as shown below (Fig. 6.14). These cover strips can easily be removed or put on.
Fig. 6.14 1.1 ml tubes in the 96-channel rack
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
29
Chapter 6: Routine Operation
6.5 How to use the control panel
The keypad has an embossed, tactile membrane with five keys. There are two control panel
indicators (LEDs) in all.
The control panel is shown in Fig. 6.15.
Fig. 6.15 Multidrop DW control panel
6.5.1 Control panel indicators
power
The green power LED indicates that the dispenser is powered.
error
The red error LED shows when the dispenser has some kind of error.
6.5.2 Control panel thumbwheels (digital switches)
volume µl
Reagent volume thumbwheels: sets the volume to be dispensed per well.
col
Column thumbwheels: sets the number of columns per plate, up to 12 with
deep-well plates, tubes in a 96-channel rack or 96-well plates.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
30
Chapter 6: Routine Operation
6.5.3 Control panel keys
start
Starts the dispensing cycle.
prime/
drop
Primes the cassette if the tips are above the priming vessel.
Primes continuously as long as the user presses the key.
Dispenses the adjusted volume if the tips are above the wells.
empty
Empties the liquid from the dispensing cassette into the liquid reservoir.
step
Advances the deep-well plate by one column.
stop
Terminates the operation and returns the plate to the home position.
6.6 How to prime
Note: Ensure that the instrument is sufficiently level on the table to prevent liquids
from overspilling when priming.
Note: Ensure that the liquid temperature is close to the calibration temperature of
22°C. Differences greater than ± 2°C may cause an error in the results.
Note: Silicon tubing (silicon rubber) is resistant to a number of chemical liquids. Most
water solutions typically used in a medical laboratory can be dispensed with the
Multidrop DW. Some strong substances may, however, harm the silicone tubing and
cause swelling or breaking. If the substance has even a slight effect on silicone, the
calibration of the cassette may be affected and the cassette may become unusable.
1.
Place the eight tube ends into the same liquid reservoir or into different reservoirs taking care
that all tube ends are below the liquid level. Use the tubing weight (Fig. 4.4,
item 7) provided if possible.
2.
Check that the priming vessel is firmly inserted next to the plate carrier. The Multidrop DW
will not operate if the priming vessel is not completely inserted into its slot.
3.
Press the prime/drop key until all eight channels are dispensing continuously into the
priming vessel and there are no more air bubbles in the tubes. We recommend you fill the
priming vessel two or three times with the reagent to be used before starting the actual
dispensing in order to bring the tubes into working condition.
Note: If you press the prime/drop key at the same time as the rotor cover is pulled
over the rotor, the program enters into the volumetric calibration mode.
Note: You can empty the contents of the priming vessel back into the reagent reservoir
by pulling the priming vessel out of its slot if necessary. We recommend you do this if
you use expensive reagents. Therefore, wash the priming vessel always after each use
to avoid any cross-contamination. Refer to Section 11.4 Consumables.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
31
Chapter 6: Routine Operation
6.7 How to check and adjust the pump rotor height for 50 mm high
plates
The pump rotor height may have to be adjusted if high (50 mm) deep-well plates are used.
Fig. 6.16 shows the adjustment screws that may have to be adjusted.
Fig. 6.16 Location of the adjustment screws that may have to be adjusted
1.
Switch off the power. The rotor cover is not pulled over the pump rotor.
2.
First place the deep-well plate straight onto the plate carrier (Fig. 6.7). Note that you must
have the dispensing cassette ready installed. Refer to Section 6.3 How to insert the
dispensing cassette and the priming vessel.
3.
Then place the black flat pump head adjustment plate supplied on top of the deep-well plate
(Fig. 6.17).
Fig. 6.17 Placing the adjustment plate onto the deep-well plate
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
32
Chapter 6: Routine Operation
4.
After that manually move the deep-well plate and the adjustment plate carefully under the
pump rotor (Fig. 6.18). Do not move the plate under the dispensing cassette against the tips
with force. The tips might get bent or deteriorated.
Fig. 6.18 Moving the deep-well plate and adjustment plate under the dispensing cassette
5.
If the deep-well plate and the adjustment plate do not fit correctly under the pump rotor, first
remove the priming vessel and place the eight tubes of the dispensing cassette on top of the
instrument. Adjust the pump rotor height by loosening either one or both of the adjustment
screws with the adjustment tool supplied (Fig. 6.19).
6.
Support the pump rotor with your hand and loosen slightly the pump head adjustment
screws (Fig. 6.19). Do not loosen the adjustment screws entirely. Loosen the screws in
succession.
Fig. 6.19 Loosening the adjustment screws
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
33
Chapter 6: Routine Operation
7.
Lift the pump rotor and manually move the deep-well plate and the adjustment plate
carefully under the dispensing cassette (Fig. 6.20), so that the tip protection pins rest on the
adjustment plate (Fig. 6.21).
The adjustment plate corresponds to the distance with which the tips and deep-well plate
must be separated. The tip protection pins rest on top of the adjustment plate when the
distance is correct. There is only an extremely narrow gap between the tips and the
adjustment plate (Fig. 6.21).
Fig. 6.20 Moving the deep-well plate under the dispensing cassette
Tip protection pins
Fig. 6.21 Tip protection pins resting on the adjustment plate
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
34
Chapter 6: Routine Operation
8.
When the pump rotor height has been adjusted, tighten the adjustment screws (Fig. 6.22).
Ensure that the pump rotor height is correct by placing the deep-well plate and the
adjustment plate again under the dispensing cassette (Fig. 6.21).
Fig. 6.22 Fastening the adjustment screws
9.
Once the pump rotor height has been adjusted correctly for the 50 mm high deep-well plate,
dispensing can proceed normally (Fig. 6.23).
Fig. 6.23 Dispensing into a deep-well plate once the pump rotor height has been adjusted
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
35
Chapter 6: Routine Operation
6.8 Dispensing
6.8.1 How to dispense automatically
1.
Set the required reagent volume per well. The permitted volume range is 20 to 995 µl for
deep-well plates, tubes in a 96-channel rack or 96-well plates.
2.
Set the required number of columns to be dispensed per plate.
3.
Place the deep-well plate straight onto the plate carrier without any adapter. However, place
the tubes in a 96-channel rack or a 96-well plate onto the plate carrier equipped with a
suitable plate adapter. Refer to Section 6.4 How to place different types of plates.
4.
Press the prime/drop key to prime the tubes. Refer to Section 6.6 How to prime.
5.
Press the start key. The Multidrop DW will now automatically dispense the preselected
volume of reagent into the preselected number of columns. When dispensing has been
completed, the plate carrier will automatically return to its home position.
6.
Now you can dispense any number of plates by only changing the plate and pressing start.
Note: Check that there is always enough liquid in the reservoir and ensure that all the
tube ends are below the liquid level. Use the tubing weight supplied.
Note: Check after each dispensing that the priming vessel has not filled up. Empty it if
necessary, otherwise the liquids may spill over during operation. Note that the
Multidrop DW always primes itself before each plate filling to ensure maximum
accuracy and precision. We recommend you empty the priming vessel after dispensing
30 to 40 plates.
6.8.2 How to dispense manually into the specified column(s)
1.
Set the desired reagent volume. The permitted volume range is 20 to 995 µl for deep-well
plates, tubes in a 96-channel rack or 96-well plates.
2.
Place the deep-well plate onto the plate carrier. Use a suitable plate adapter for tubes in a
96-channel rack or 96-well plates.
3.
Use the step key to move the plate carrier column by column until you reach the desired
column.
4.
Press the prime/drop key. The Multidrop DW will now dispense the preselected volume
into the column desired. The plate carrier will return to the home position after reaching the
last column. If you use the start key, the instrument will automatically dispense the selected
volume from the set position to the last column selected.
Note: If you have pressed either the stop or empty key, the Multidrop DW refuses to
start dispensing before you have pressed the prime/drop key once again.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
36
Chapter 6: R outi ne O per ati on
6.8.3 How to dispense multiple volumes
Ten- or hundredfold volumes can be dispensed compared to the volume chosen.
6.8.3.1 Dispensing tenfold and hundredfold volumes
1.
Press the stop and prime/drop keys simultaneously.
2.
Having done that first, release the stop key and then the prime/drop key. Note that the
Multidrop DW is in the volumetric calibration mode.
3.
Now you can push the plate carrier into the desired dispensing position.
4.
Set one tenth of the volume required on the thumbwheel. Then press the start key and a
tenfold volume will be dispensed.
5.
Set one hundredth of the volume required on the thumbwheel. Then press the step key and
a hundredfold volume will be dispensed.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
37
Chapter 6: Routine Operation
6.8.4 After use
After all the plates have been dispensed, press the empty key to return the reagent or liquid from
the tubes to the reservoir. This saves expensive reagents.
If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the empty key, release the tubing tension by pulling the upper part of
the cassette from its slots and place it into its rest position slots located on the left-hand side of
the rotor (Fig. 6.24).
If there is a longer break (for example, a day), wash the dispensing cassette by priming it with
deionized distilled water or with special washing detergent, if necessary. In the end, rinse the
washing detergent away with deionized distilled water.
After washing, store the dispensing cassette in the rest position (Fig. 6.24).
Note: Keep the dispensing cassette in the rest position (Fig. 6.24) when the dispensing
cassette is not in use.
Fig. 6.24 Rest position of the dispensing cassette
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
38
Chapter 6: Routine Operation
6.9 Shutdown
Remove any deep-well plates still on the plate carrier. Dispose of all deep-well plates,
tubes in a 96-channel rack, 96-well plates and microstrips as biohazardous waste.
After all the deep-well plates have been dispensed, press the empty key to return the reagent or
liquid from the tubes to the reservoir. This saves expensive reagents.
If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the empty key, release the tubing tension by pulling the upper part of
the cassette from its slots and place it into its rest position slots located on the left-hand side of
the rotor (Fig. 6.24).
If there is a longer break (for example, a day), wash the dispensing cassette by priming it with
deionized distilled water or with special washing detergent followed by extensive washing with
deionized distilled water, if necessary.
After washing, store the dispensing cassette in the rest position (Fig. 6.24).
Switch the Multidrop DW off by pressing the mains switch (Fig. 4.2) at the end of the left side
panel of the instrument into the OFF position.
Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled
water, a mild detergent (SDS, sodium dodecyl sulfate) or soap solution.
If you have spilt infectious agents on the dispenser, disinfect with 70% alcohol or some other
disinfectant (see Section 7.8 Decontamination procedure).
Last of all put the dust cover on.
6.10 Guidelines for use
•
When the error LED is on, the start or step keys will not function.
•
To clear the error and continue the dispensing procedure, press the stop key and wait until
the plate carrier has returned to the home position. The dispensing procedure can then be
restarted.
•
If you press the stop key, all operations will stop. The plate carrier is automatically returned
to the home position. The Multidrop DW will now require priming again.
•
Preventative maintenance should be followed closely to keep the deep-well plate dispenser in the
best condition for maximum reliability. A poorly maintained instrument will not give the best
results.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
39
Chapter 7: Maintenance
7 Maintenance
7.1 Regular and preventive maintenance
7.1.1 General
Routine and service procedures must be performed by the user to prevent unnecessary wear or
hazards and are described below at the frequency with which they should be applied.
Abrasive cleaning agents are not recommended, because they are likely to damage the paint
finish.
We recommend you clean the case of the instrument periodically to maintain its good
appearance. A soft cloth dampened in a warm, mild detergent solution will be sufficient.
Clean the outside of the instrument and the plate carrier with clean low-pressure compressed air
or a cloth dampened with water or a mild detergent when necessary.
Painted surfaces can be cleaned with most laboratory detergents. Dilute the cleaning
agent as recommended by the manufacturer. Do not expose painted surfaces to
concentrated acids or alcohols for prolonged periods of time as damage may occur.
Clean the control panel with a mild laboratory detergent.
Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol.
If any surfaces have been contaminated with biohazardous material, a mild sterilizing
solution should be used.
The dispensing cassette is autoclavable. Do not autoclave any other part of this
instrument.
7.1.2 Immediate
Although the Multidrop DW is constructed from high-quality materials, you must immediately
wipe away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to
prevent damage and wipe with deionized distilled water.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
40
Chapter 7: Maintenance
7.2 How to wash the dispensing cassette
Wash the tubes by priming them with deionized distilled water. Make sure all the tubes are
washed properly.
If cleaning with water is insufficient, use a mild laboratory detergent and then prime with large
amounts of deionized distilled water.
The dispensing cassette can be dried at room temperature.
After washing, store the dispensing cassette in the rest position (Fig. 6.24).
If the instrument is not used for a couple of hours, insert the dispensing cassette into the rest
position (Fig. 6.24). Refer to Section 6.8.4 After use. Handle the dispensing cassette with great
care so that you do not damage the tubes and dispensing tips. These factors will affect the useful
life of the dispensing cassette.
7.3 How to autoclave the dispensing cassette
The dispensing cassette can be autoclaved 50 times with the same tubing set depending on the
reagent used.
The autoclaving conditions of the dispensing cassette are as follows: 1 bar pressure at 121°C for
20 minutes.
Note: After autoclaving, the dispensing cassette must cool down to room temperature
before use.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
41
Chapter 7: Maintenance
7.4 How to replace the fuses
1.
Switch off (Fig. 4.2) and unplug the instrument from the mains input socket (Fig. 4.5).
2.
Remove the fuse holder cap with a screwdriver or similar tool. Press both ends of the fuse
holder cap with the tool (Fig. 7.1 and Fig. 7.2).
Fig. 7.1 Removing the fuse holder cap (A)
Fig. 7.2 Removing the fuse holder cap (B)
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
42
Chapter 7: Maintenance
3.
Check the fuses in the fuse holder cap (Fig. 7.3).
Fig. 7.3 Fuse holder cap with fuses
4.
Replace the blown fuse with the same type and push the fuse holder cap back into its
position (Fig. 7.4).
Fig. 7.4 Inserting the fuse holder cap
5.
Reconnect to the mains input socket and switch the power on.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
43
Chapter 7: Maintenance
7.5 How to recalibrate the dispensing cassette
The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at
22°C ± 2°C. We recommend you recalibrate the cassette after dispensing about 1000 96-well
plates or after every three months. We also recommend you recalibrate the dispensing cassette if
you have forgotten the cassette in the Multidrop DW under tension for prolonged periods, for
example, a weekend or longer.
Two different calibration methods are described below, that is, volumetric calibration and
gravimetric calibration.
7.5.1 Volumetric calibration
For volumetric calibration, use the following steps:
1. Remove the priming vessel and deep-well plate.
2. Install the dispensing cassette into the Multidrop DW if it is not already installed. Place the
dispensing cassette into the rest position (Fig. 6.24). Refer to Section 6.3 How to insert the
dispensing cassette and the priming vessel.
3. Connect the two parts of the volumetric calibration device (Cat. no. 2406910) by inserting the
syringe firmly into its slot in the calibration pipette. Then install the tube of the volumetric
calibration device into the tip of the channel to be recalibrated (Fig. 7.5), for example, A.
Fig. 7.5 Placing the tube onto the tip of channel A
4. Insert the dispensing cassette into the working position so that the lower and upper parts of
the dispensing cassette are inserted into their slots (Fig. 6.4).
5. Pull the rotor cover over the rotor.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
44
Chapter 7: Maintenance
6. Place the tube of channel A into the reservoir with water at room temperature (working
temperature).
7. Open the calibration screw cover on the upper part of the cassette (Fig. 7.7 and Fig. 4.4).
8. Set the volume to 100 µl.
9. Press the stop and prime/drop keys at the same time. After that first release the stop key
and then the prime/drop key. This brings the Multidrop DW into the volumetric calibration
mode.
10. Press the start key as many times until the water enters the calibration pipette.
Note: Take care not to press the step key accidentally or otherwise 10 ml of water will
be dispensed.
11. Adjust the water level in the pipette to the lower mark by pulling or pushing the syringe piston
(Fig. 7.6).
Upper mark
Lower mark
Fig. 7.6 Adjusting the liquid level first to the lower mark and then to the upper mark
12. Press the start key and wait until the water level stops. It should be at the upper mark,
1000 µl (± 1.5%) (Fig. 7.6).
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
45
Chapter 7: Maintenance
13. If the volume is higher than 1000 µl, turn the calibration screw (Fig. 7.7) clockwise with a
screwdriver; one turn for each 10 µl (1% from the volume dispensed); if the volume is lower
than 1000 µl, turn in the opposite direction.
Calibration screw
cover opened
Fig. 7.7 Screwing the calibration screws according to the calibration results
14. Press the empty key and adjust the water level in the pipette to the lower mark and repeat
Steps 10 to 13 until the result is 1 ml. Three accurate calibrations must be obtained.
15. Change the tube to the next channel and repeat Steps 10 to 14.
16. After all the channels have been recalibrated, press the stop key to reset the Multidrop DW
into normal operation and reinstall the priming vessel.
17. Close the calibration screw cover.
7.5.2 Gravimetric calibration
For gravimetric calibration, use the following steps:
1.
Install the dispensing cassette into the working position so that the lower and upper parts of
the dispensing cassette are inserted into their slots (Fig. 6.4).
2.
Pull the rotor cover over the rotor.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
46
Chapter 7: Maintenance
3.
Ensure that the priming vessel is installed. Refer to Section 6.3 How to insert the dispensing
cassette and the priming vessel.
4.
Ensure that you have the plate adapter for 96-well plates installed (Fig. 6.9). Refer to Section
6.4 How to place different types of plates.
Note: Keep the dispensing cassette in the rest position (Fig. 6.24) when the dispensing
cassette is not in use.
5.
Place the tube of channel A into the reservoir with water at room temperature (working
temperature).
6.
Use 8 x 12 well microstrips and adjust the Multidrop DW for 100 µl in 10 columns (Fig. 7.8).
Fig. 7.8 8 x 12 well microstrips adjusted onto the plate adapter
7.
Press the prime/drop key to prime the tubes. Refer to Section 6.6 How to prime.
8.
Open the calibration screw cover on the upper part of the cassette.
9.
Tare an analytical balance (resolution 0.01 mg) with one empty, dry 1 x 12 well microstrip.
10. Place the microstrip into the microplate frame on the row corresponding to the channel to be
recalibrated (from A to H). Press the start key.
11. Weigh the filled microstrip (Fig. 7.9). The balance should now read 1000 mg ± 20 mg.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
47
Chapter 7: Maintenance
Fig. 7.9 Removing the filled microstrip to be weighed
12. If the balance reads less than 980 mg, turn the calibration screw counterclockwise: one turn
for each 10 mg (1% from the volume dispensed). If the balance reads more than 1020 mg,
turn in the opposite direction (Fig. 7.10). Recheck the delivery after each adjustment using
Steps 9 to 11. Three acceptable calibrations must be obtained.
Fig. 7.10 Screwing the calibration screw according to the calibration results
13. Continue in the same way with each channel until all eight channels have been recalibrated.
14. Close the calibration screw cover.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
48
Chapter 7: Maintenance
7.6 How to change the tubing set
Note:
You can also order a complete calibrated dispensing cassette, thereby eliminating
the need to change the tubing set (see Section 11.2 Dispensing cassettes).
7.6.1 Changing the tip band
1.
Place the dispensing cassette onto the laboratory bench with the dispensing tips pointing
down.
2.
Unfasten the four cover retaining screws of the lower part of the dispensing cassette
(Fig. 7.11).
Fig. 7.11 Removing the cover plate of the lower part of the dispensing cassette
3.
Remove the tip manifold, the tube fasteners and the rest of the tubing from the lower part of
the dispensing cassette. Then detach the tip band from the tubing by pulling the tubes away
from the tip band (Fig. 7.12).
Fig. 7.12 Removing the old tip band
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
49
Chapter 7: Maintenance
4.
Replace the tip band (Fig. 7.13). The tips of the tip band must not be clogged in any way but
be clean to function correctly (Fig. 7.14).
Fig. 7.13 Tip band removed
Insert tube
Tip
Fig. 7.14 New tip band
7.6.2 Fitting the new tubing set step by step
1.
First place the new tubing set onto the laboratory bench with the tip band attached (Fig.
7.15). Ensure that you have saved the eight calibration screws (Fig. 7.21 and Fig. 7.25) and
the tubing weight (Fig. 4.4, item 7 and Fig. 7.35) from the previous tubing set.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
50
Chapter 7: Maintenance
Tubing identification
plate
Fig. 7.15 New tubing set with the tip band attached
2.
Remove the four cover retaining screws on the lower part of the dispensing cassette, the
tubes with the tube fasteners and the tip manifold with the tips, the three cover retaining
screws on the upper part of the dispensing cassette, the two tension limiting wires, the
calibration screws and the tubes with the tube fasteners. Save the calibration screws and the
tubing weight for further use when installing the new tubing set.
3.
Insert the tip manifold so that the tips go through the holes in the lower part of the
dispensing cassette (Fig. 7.16) and guide the tubes through the eight openings in the side
wall by pressing the small cubic tube fasteners into place (Fig. 7.17). Ensure that each tube is
in its designated opening (Fig. 7.18).
Tip band
Tube fasteners
Fig. 7.16 Inserting the tip manifold into the lower part of the dispensing cassette
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
51
Chapter 7: Maintenance
Fig. 7.17 Pressing the tube fasteners into place
Fig. 7.18 New tubing inserted into the lower part of the dispensing cassette
4.
Attach the tension limiting wires into their slots (Fig. 7.19).
Slots for tension limiting wires
Fig. 7.19 Tension limiting wires attached
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
52
Chapter 7: Maintenance
5.
Fasten the four cover retaining screws of the lower part of the dispensing cassette (Fig. 7.20).
Fig. 7.20 Replacing the lower part cover of the dispensing cassette
6.
Once the lower part of the dispensing cassette has been fitted, place the remaining parts
belonging to the upper part of the dispensing cassette onto the laboratory bench (Fig. 7.21).
Fig. 7.21 Parts belonging to the upper part of the dispensing cassette
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
53
Chapter 7: Maintenance
7.
First place the plastic sight window with the grooves pointing upwards onto the bottom
cover of the upper part of the dispensing cassette, if it is not already there (Fig. 7.22).
Fig. 7.22 Placing the sight window
8.
After that press the white tubing identification plate into place on the right-hand side of the
bottom cover of the upper part of the dispensing cassette (Fig. 7.23).
Fig. 7.23 Pressing the tubing identification plate into place
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
54
Chapter 7: Maintenance
9.
Then press each tube fastener into place in its groove on the plastic sight window (Fig. 7.24).
Fig. 7.24 Pressing the tube fasteners into place
10. Fasten the calibration screws from the previous tubing into their places using a hexagonal
screwdriver (Fig. 7.25).
Fig. 7.25 Fastening the calibration screws
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
55
Chapter 7: Maintenance
11. Insert the tension limiting wires into their slots into the upper part of the dispensing cassette
(Fig. 7.26).
Slots for tension limiting wires
Fig. 7.26 Calibration screws fastened
12. Replace the upper part cover of the dispensing cassette carefully (Fig. 7.27).
Fig. 7.27 Replacing the cover of the upper part of the dispensing cassette
13. Ensure that you do not get the tubing trapped or bent between the bottom and upper cover
of the upper part of the dispensing cassette (Fig. 7.28).
Fig. 7.28 Ensuring the tubing does not get trapped
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
56
Chapter 7: Maintenance
14. Fasten the three cover retaining screws of the upper part of the dispensing cassette, leaving
the front screw slightly open (Fig. 7.29).
Fig. 7.29 Fastening the cover retaining screws
15. Slide the calibration screw cover easier by lifting or bending the upper cover slightly
(Fig. 7.30).
Fig. 7.30 Sliding the plate onto the calibration screws
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
57
Chapter 7: Maintenance
16. Fasten the front cover retaining screw (Fig. 7.31).
Fig. 7.31 Lower and upper part of the dispensing cassette fitted
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
58
Chapter 7: Maintenance
17. Turn the dispensing cassette so that the eight sight strips are visible (Fig. 7.32). Look at the
eight sight strips in the upper part of the dispensing cassette. You will notice that the tubes
are not precalibrated. First open the calibration screw cover. For precalibration of the
dispensing cassette, adjust the tube fasteners in the sight strips roughly to the middle using a
hexagonal screwdriver (Fig. 7.33).
Sight strips
Fig. 7.32 Dispensing cassette before precalibration
Fig. 7.33 Precalibrating the dispensing cassette
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
59
Chapter 7: Maintenance
18. When the tube fasteners have all been adjusted roughly to the middle of the sight strip, close
the calibration screw cover (Fig. 7.34).
Fig. 7.34 Dispensing cassette precalibrated
19. Insert the eight tubes into the tubing weight either at this stage or earlier (Fig. 7.35). The new
tubing set has now been successfully fitted.
Fig. 7.35 Inserting the tubes into the tubing weight
20. Calibrate the dispensing cassette according to the instructions in Section 7.5 How to
recalibrate the dispensing cassette.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
60
Chapter 7: Maintenance
7.7 Disposal of materials
Refer to local regulations for the disposal of infectious material.
The samples can be potentially infectious. Dispose of all used deep-well plates, tubes
in a 96-channel rack, 96-well plates, microstrips, tip bands, disposable gloves,
syringes, disposable tips, etc. as biohazardous waste.
7.8 Decontamination procedure
Decontamination should be performed in accordance with normal laboratory procedures. Any
decontamination instructions provided with the reagents used should be followed.
A decontamination procedure is only recommendable when infectious substances have been in
direct contact with any part(s) of the instrument.
If there is any risk of contamination with biohazardous material, the procedure recommended
below or some other corresponding efficient decontamination procedure must be performed.
You must perform the complete decontamination procedure before shipping the instrument to
Thermo Fisher Scientific Oy, for example, for repair, or relocating the instrument from one
laboratory to another. Refer to Section 7.10 How to pack for service. Decontamination is not
required for the proper functioning of the instrument.
Example of decontaminants
•
•
•
•
•
Ethanol
Virkon solution
Glutaraldehyde solution
Chloramine T
Microcide SQ
70%
1 ― 3%
4%
1:64
IF LOCAL OR LABORATORY REGULATIONS PRESCRIBE REGULAR DISINFECTION, IT
IS NOT ADVISABLE TO USE FORMALDEHYDE, SINCE EVEN SMALL TRACES OF
FORMALDEHYDE AFFECT THE ENZYME BEING USED IN EIA TESTS IN A NEGATIVE
WAY RESULTING IN BAD TEST RESULTS.
Always use disposable gloves and protective clothing and operate in a well-ventilated
area.
Do not autoclave any other parts of the instrument except the dispensing cassette.
1.
Prepare the decontaminant: for example, 1 ― 3% Virkon solution or 200 ml 4%
glutaraldehyde solution (or another agent recommended by your safety officer).
2.
Empty the reagent container.
3.
Switch the power OFF and disconnect the mains supply cable (Fig. 4.2).
4.
Disinfect the outside of the instrument using a wad of cotton wool/cloth soaked in 70%
ethanol.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
61
Chapter 7: Maintenance
5.
Place the plate adapter and dispensing cassette, for example, in 1 ― 3% Virkon solution or
200 ml 4% glutaraldehyde solution for 10 min and rinse them with deionized distilled water.
6.
Place the entire instrument in a large plastic bag. Ensure that the reagent containers are
disconnected and open.
7.
Place a wad of cotton wool soaked in the prepared solution into the bag. Ensure that the wad
does not make contact with the instrument.
8.
Close the bag firmly and leave the instrument in the bag for at least 24 hours.
9.
Remove the instrument from the bag.
10. Clean the instrument using a mild detergent.
11. Remove any stains using 70% ethanol.
12. Flush the fluid path of the instrument with deionized distilled water using the priming
procedure.
13. The dispensing cassette must be autoclaved.
14. Also wash and disinfect the reagent containers.
15. After performing this decontamination procedure, enclose a signed and dated Certificate of
Decontamination both inside the transport package and attached to the outside of the
package.
7.9 Maintaining a system log
A system log, which includes a short summary of the use, maintenance procedures, error
messages and other information about the use of the system can be very useful in properly
maintaining the system. The information in the log can frequently provide the service engineer
with information that can assist in the diagnosis of problems and minimize the down time. An
example of a typical user log is presented in the following table.
The format of the log can vary to meet the overall requirements of the facility but should include
all activity, problems, abnormal response and any other information that is relevant to the
operation of the system.
User
Date
Date of calibration /
dispensing cassette change /
tubing set change
Liquid dispensed
# Plates run
Comments
J. Smith
3/6/07
_
PBS
41
OK
R. Jones
3/6/07
_
DMSO
5
OK
C. Mayo
3/8/07
_
PBS-Tween
26
J. Smith
3/9/07
New disp. cassette 3/9/07
7:16 PM
PBS
75
Used new
liquid
OK
A blank system log table that can be copied for use can be found in Appendix A. System Log.
Copy the table as many times as needed but leave the blank original inside the user manual.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
62
Chapter 7: Maintenance
7.10 How to pack for service
When you ship the instrument for service remember to:
•
•
•
•
•
•
•
Inform about the use of hazardous materials.
Decontaminate the instrument beforehand.
Pack the instrument according to the enclosed packing instructions.
Use the original packaging to ensure that no damage will occur to the instrument during
shipping. Any damage will incur additional labor charges.
Enclose a dated and signed Certificate of Decontamination (see Section 9.5) both inside and
attached to the outside of the package, in which you return your instrument (or other items).
Enclose the return authorization number (RGA) given by the Thermo Fisher Scientific
representative.
Indicate the fault after you have been in touch with your local Thermo Fisher Scientific
representative or the Thermo Fisher Scientific technical service department.
See Section 8.1 for details on storage and transportation temperatures.
7.11 Service contracts
We recommend you maintain and service the instrument regularly every twelve months on a
contract basis by the manufacturer's trained service engineers. This will ensure that the product is
properly maintained and gives trouble-free service. Contact the Thermo Fisher Scientific technical
service department for more details.
7.12 Disposal of the instrument
•
Decontaminate the instrument prior to disposal. See Sections 7.8 and 9.5 on
decontamination. Also wash and disinfect the plate adapters and the dispensing
cassettes prior to disposal.
•
Dispose of the instrument according to the legislation stipulated by the local
authorities concerning take-back of electronic equipment and waste. The proposals
for the procedures vary by country.
Thermo Fisher Scientific has contracted with one or more recycling/disposal
companies in each EU Member State (European Country), and this product should
be disposed of or recycled through them. Further information on Thermo Fisher
Scientific’s compliance with these Directives, the recyclers in your country, and
information on Thermo Scientific products which may assist the detection of
substances
subject
to
the
RoHS
Directive
are
available
at
www.thermo.com/WEEERoHS.
•
Regarding the original packaging and packing materials, use the recycling operators
known to you.
•
Further information can be obtained from your local Thermo Fisher Scientific office
or local supplier.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
63
Chapter 8: Technical Specifications
8 Technical Specifications
8.1 General specifications
Overall dimensions
310 mm (12.2 in.) (W) x 320 mm (12.6 in.) (D) x 180 mm (7.1 in.) (H)
Weight (total)
Instrument
Mains power supply
100 ― 120 Vac, 50/60 Hz, nominal (operating range 90 ― 130 Vac)
200 ― 240 Vac, 50/60 Hz, nominal (operating range 180 ― 256 Vac)
Power consumption
Heat dissipation
50 VA max.
171 BTU max.
Fuses
2 x 300 mA for 200 ― 240 V, UL 198G Time Delay, 5 x 20 mm
2 x 600 mA for 100 ― 120 V, UL 198G Time Delay, 5 x 20 mm
Operating conditions
(indoor use)
+10°C to +40°C, packed in transport packaging
Transportation
conditions
Storage conditions
Autoclaving conditions
of the dispensing
cassette
-40°C to +70°C, packed in transport packaging
Control panel
Keys: start, prime/drop, empty, step, stop
Selection switches: volume µl, col (= column)
Interface
Plates
Serial RS-232C
96-well deep-well plates
96-well microplates
Micronic 96-place tube racks
1.1 ml tubes in a 96-channel rack
Dispensing volume
20 – 995 µl in 5 µl increments
Number of channels
Dispensing speed
Eight
5 s/20 µl into 96 wells
11 s/100 µl into 96 wells
26 s/300 µl into 96 wells
51 s/600 µl into deep-well plates
74 s/900 µl into deep-well plates
Dispensing accuracy
± 3% or ± 1 µl, whichever is greater
20 µl: ± 2% (typical*)
100 µl: ± 1% (typical*)
Valid when counted for the entire plate
20 ― 995 µl: CV ≤ 3.0% or SD ≤ 0.4 µl, whichever is greater
20 µl: ≤ 1.5% (typical)
100 µl: ≤ 1% (typical)
900 µl: ≤ 0.5% (typical)
Valid when counted for the entire plate
Dispensing precision
6.2 kg (13.7 lbs.)
Dispensing cassette
124 g (0.3 lb.)
-25°C to +50°C, packed in transport packaging
pressure: 1 bar
temperature: 121°C
time: 20 minutes
autoclavings: 50 times
* using the calibration procedure in Section 7.5.1 the accuracy can be improved to ± 1.5%
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
64
Chapter 8: Technical Specifications
8.2 Safety specifications
8.2.1 Live parts
The instrument is safe to operate with the covers fitted and these must not be removed during
operation. The covers protect the user from live parts and they should only be removed after
switching the instrument off and disconnecting the mains supply cable, and only by suitably
qualified maintenance and repair personnel.
Voltages dangerous for human beings are present in this instrument. Before removing
any covers, disconnect the instrument from the power supply.
The Multidrop DW fulfils the following requirements:
EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995
taking into account US and CA National differences
EN 61010-1:2001 (Ed. 2)
The safety specifications are also met under the following environmental conditions in addition to
or in excess of those stated in the operating conditions:
Altitude
Temperature
Mains supply fluctuations
Installation category (overvoltage category)
Pollution degree
up to 2000 m
+5°C ― +40°C
± 10% from nominal
II according to IEC 60664-1 (see Note 1)
2 according to IEC 60664-1 (see Note 2)
Notes
1. The installation category (overvoltage category) defines the level of transient overvoltage,
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its means of overvoltage protection. For example, in CAT II, which is
the category used for instruments in installations supplied from a supply comparable to public
mains, such as hospital and research laboratories and most industrial laboratories, the
expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.
2. The pollution degree describes the amount of conductive pollution present in the operating
environment. Pollution degree 2 assumes that normally only nonconductive pollution, such as
dust, occurs with the exception of occasional conductivity caused by condensation.
Both of these affect the dimensioning of the electrical insulation within the instrument.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
65
Chapter 8: Technical Specifications
8.3 In conformity with the requirements
The Multidrop DW bears the following markings:
Type 833
100 ― 120 Vac, 200 ― 240 Vac 50/60 Hz, 50 VA
CE mark
CSA monogram
The Multidrop DW conforms to the following requirements:
2006/95/EC (Low Voltage Directive)
2004/108/EC (Electromagnetic Compatibility Directive, EMC)
FCC Part 15, Subpart B/Class B
2002/96/EC (Waste of Electrical and Electronic Equipment)
Safety performance:
EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995
taking into account US and CA National differences
EN 61010-1:2001 (Ed. 2)
EMC performance:
EN 50081-1:1992
Generic emission standard. Residential, commercial and light industry.
EN 50082-1:1997
Generic immunity standard. Residential, commercial and light industry.
EN 61326-1:1997 + A1:1998 Product family standard.
+ A2:2001 + A3:2003
Test standards
Performance limits
EN 55022:1998
Class B, 150 kHz – 1 GHz
EN 61000-3-2:1995 + A1:1998 +
A2:1998 + A13:1997 + A14:2000
EN 61000-3-3:1995
ANSI C63.4:1992
Class A
EN 61000-4-2:1995 + A1:1998
EN 61000-4-3:1996 + A1:1998
EN 61000-4-4:1995
EN 61000-4-5:1995
EN 61000-4-6:1996
EN 61000-4-8:1994
EN 61000-4-11:1994
4 kV CD, 8 kV AD, Criteria B
3 V/m, 80 MHz ― 2 GHz, Criteria A
1 kV, Criteria B
2 kV line to ground, 1 kV line to line, Criteria B
3 Vrms, 150 kHz ― 80 MHz, Criteria A
3 A/m, Criteria A
30%/10 ms, Criteria B
60%/100 ms, Criteria C
100%/20 ms, Criteria B
> 95%/5 s, Criteria C
Class B, 450 kHz – 1 GHz
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
66
Chapter 8: Technical Specifications
8.4 Remote control to Multidrop DW
The interface system consists of the serial RS-232C computer port which is used for computer
control.
8.4.1 Hardware for the computer port
The character length is 10 bits with 1 start bit, 8 data bits, 1 stop bit and no parity. The format
cannot be changed.
The following signals are connected to the computer port DB-25 connector at the rear of the
instrument:
DB-25 CONNECTOR
Pin no.
Signal name
2
TxD
3
RxD
4
RTS
7
GND
20
DTR
Signal description
2:
3:
7:
4/20:
TxD, transmit data (output)
RxD, receive data (input)
GND, signal ground
RTS/DTR (handshake lines)
The handshake lines:
RTS/DTR are not in use in the standard Multidrop DW.
8.4.2 Multidrop DW PC interface
Baudrate
Parity
Data bits
Stop bits
Handshake
9600
None
8
1
XON/XOFF
Commands
Commands to the Multidrop DW have two to three fields as described in the following syntax. The
first letter identifies the command. Then a numeric argument field is optional for some and
required for other commands. The numeric field immediately follows the uppercase letter without
any separating characters. The last character of a command is a linefeed character <LF> or a
carriage return character <CR>. Commands may also be terminated to <CR><LF> or
<LF><CR>, since the instrument ignores empty commands.
Note:
The Multidrop 384 microplate dispenser contains the same program version
and commands as the Multidrop DW. Therefore, the Multidrop 384 commands
are only valid with the Multidrop 384 microplate dispenser.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
67
Chapter 8: Technical Specifications
In the following list of commands, the command arguments in square brackets are optional.
D<LF>
Dispense the volume set by the “V” command to the entire plate.
Primes 10 µl into the priming vessel before dispensing.
E<LF>
Empty the pump. A volume of 880 µl is pumped backwards.
G<LF>
Dispense the plate using parameters from the plate switch and the volume/columns
thumbwheels. This command has the same effect as pressing the start key. This
command has been added to version 1.7.
M[c]<LF>
Dispense the given number of columns starting from the current column. If the
column count is not given, one column is dispensed. The dispensing volume is set
by the “V” command. After the command is completed, the dispensing tips remain at
the last column dispensed. If this command is received when the plate is in the home
position, dispensing starts from column 1. ER3 is reported if more columns are
requested than left over from the current column to the last column of the plate.
N<LF>
Report the internal software version of the instrument.
O<LF>
Drive the plate out to the priming position.
P[v]<LF>
Prime the given volume. For a 96-well plate the volume must be in the range of 5 to
1000 µl. For a 384-well plate the range is 5 to 100 µl. The volume must be dispensed
in 5 µl increments. If the volume is not given, 200 µl is primed. If the plate is not in the
home position when the command is received, it is first driven into home position.
Q<LF>
Reset the instrument. There is no response to this command.
S[c]<LF>
Drive the requested column under the dispensing tips. Valid columns are 1 to 12 for
a 96-well plate and 1 to 24 for a 384-well plate. If the column is not given, the steps
are one column forward or the plate returns to home position after the last column.
Tt<LF>
Set the plate type assumed for all other commands except the “G” command. At
startup the default type is read from the plate switch.
t = 0 96-well plate
t = 1 384-well plate
This command has been added to version 1.7.
V<LF>
VER<LF>
Report the internal software version of the instrument just like the “N” command.
Vv<LF>
Sets the volume for dispensing. For a 96-well plate the volume must be in the range
of 5 to 1000 µl. For a 384-well plate the range is 5 to 100 µl. The volume must be
dispensed in 5 µl increments.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
68
Chapter 8: Technical Specifications
Responses and error codes
The instrument sends a response to each command after the command has been executed. The
only exception is the “Q” command, for which there is no response.
The response to a “N” command is as follows:
MdropDW Vr.l[-b]<CR><LF>
where:
r is the release number;
l is the level number, and
b is the optional branch number.
The response to other commands, if the command is executed successfully, is as follows:
OK<CR><LF>
If an error is detected, the response is one of the following:
ER3<CR><LF>
ER4<CR><LF>
ER5<CR><LF>
ER6<CR><LF>
Unrecognized command or invalid command argument.
The pump is not primed.
The priming vessel is not inserted into its slot.
Hardware error. The instrument stops and must be reset manually.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
69
Chapter 9: Troubleshooting
9 Troubleshooting
9.1 Troubleshooting guide
The problems covered below are considered as faults that require repair or corrective work. If the
installation procedure is carefully followed, no faults should arise. However, if problems do occur
or reoccur, contact authorized technical service immediately.
Error
Cause
Action
No power to the
instrument
The instrument has not been connected to the
mains.
Connect the instrument to the mains.
A fuse has blown.
Replace the blown fuse (see Section 7.4).
Check that the rotor cover is over the
rotor.
The keys do not function
Drop on the end of the tip
exceptionally large
Large air bubbles in the
tubes
Poor dispensing accuracy
and precision
The tip is defective or clogged.
The tube is defective.
Replace the dispensing cassette or the
tubes.
The dispensing cassette is not properly
installed.
The instrument is not primed.
Reinstall the cassette.
The dispensing cassette is not properly
installed.
Reinstall the cassette.
The tubes are worn out.
Replace the dispensing cassette or the
tubes.
There is friction on one of the rotor needles.
Bearing malfunction.
The rotor needles should spin without any
friction. If even a slight friction is present,
contact authorized technical service.
Reinstall the cassette.
Liquid being dispensed
does not fall into the
required well of the deepwell plate
The dispensing cassette is not properly
installed.
Dispensing does not start
The deep-well plate hits the tip ends or the tips
do not hit the well.
There is foreign matter in the way of the
carrier.
Plate carrier moves
incorrectly
Instrument does not
function
The error LED operates
when trying to prime
Check that the priming vessel is properly
inserted into its slot.
Clear the tip with a needle or replace the
tip strip.
The tip is defective or clogged.
Prime (see Section 6.6).
Clear the tip with a needle or replace the
tip strip.
Adjust the height of the pump module.
Remove the foreign matter.
The drive band of the drive wheel is worn out
or broken.
Contact authorized technical service.
The drive wheel is loose.
Contact authorized technical service.
The drive wheel is not properly adjusted.
Contact authorized technical service.
Contact authorized technical service.
The priming vessel is not present.
Insert the priming vessel.
There is dirt in the priming vessel sensor.
Clean the priming vessel sensor.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
70
Chapter 9: Troubleshooting
9.2 Frequently asked questions (FAQ) about the Multidrop DW
Q1: Can the same dispensing cassette be used in all Multidrop models?
A1: No, use only dispensing cassettes designed for Multidrop DW. It is not possible to use small
tube dispensing cassettes.
Q2: How many times can the dispensing cassette be autoclaved?
A2: Fifty times.
Q3: What are the autoclaving conditions for the dispensing cassette?
A3: The autoclaving conditions are as follows: 1 bar pressure at 121°C for 20 minutes.
Q4: What is the dead volume of the standard 40 cm tubing set?
A4: The dead volume is about 5.6 ml. Note that you do not waste the reagent due to the special
backflush feature of the Multidrop DW, with which you can empty your reagents from the tubing
back into the original container.
Q5: What liquid is recommended to be used for washing the Multidrop DW tubes?
A5: We recommend you use deionized distilled water and/or detergent solutions, for example,
0.2 – 1% Tween followed by deionized distilled water.
Q6: What is the material of the Multidrop DW priming vessel?
A6: The Multidrop DW priming vessel is made of black polyvinylchloride (PVC).
Q7: Can the priming vessel be autoclaved?
A7: No, it cannot be autoclaved.
Q8: What is the material of the dispensing cassette tip band?
A8: The tip band (Fig. 7.14) material is polypropylene (PP). The melting temperature of the
material is 160 to 165°C.
Q9: How long can the tubes be used?
A9: We recommend you change the tubing set after dispensing about 3000 to 5000 96-well plates
depending on the dispensing frequency and the liquid used.
Q10: Can ultraviolet light be used for sterilization?
A10: Ultraviolet rays can be used to sterilize objects, for example, the surface of the Multidrop DW
instrument and other areas where destruction of bacteria is necessary.
Q11: When should I calibrate the dispensing cassette?
A11: The dispensing cassette needs to be calibrated when the silicone tubes have been changed.
We also recommend you recalibrate the dispensing cassette after dispensing about a 1000 96-well
plates or after every three months. The dispensing cassette must also be recalibrated if it has been
forgotten under tension for prolonged periods, for example, a weekend or longer.
Q12: What is the dead volume of the 2 m tubing set?
A12: The dead volume is about 24 ml.
Q13: How high deep-well plates can be used?
A13: Up to 50 mm high deep-well plates can be used.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
71
Chapter 9: Troubleshooting
9.3 Hazards
This instrument is designed to provide full user protection. When correctly installed, operated and
maintained, the instrument will present no hazards to the user.
The following recommendations are given to ascertain added user safety.
9.3.1 Electrical
Ensure that the mains supply cable supplied with the unit is always used. If a correct type of
mains cable is not provided, use only cables certified by the local authorities.
The mains plug should only be inserted into a socket outlet provided with a protective ground
contact. The protective action must not be negated by use of an extension cable without a
protective ground wire.
When the instrument is connected to the mains supply, the opening of covers or removal of
components is likely to expose live parts. The instrument should be disconnected from all voltage
sources by disconnecting the mains supply cable, before it is opened for any adjustment,
replacement, maintenance or repair purposes.
Any adjustment, maintenance or repair of the opened instrument under voltage should be
avoided, if possible, but if unavoidable, should only be carried out by a skilled technician aware of
the hazard.
Voltages dangerous for human beings are present in this instrument. Before removing
any covers, disconnect the instrument from the power supply.
The same precautions applicable when using any electrical equipment should certainly be
observed with this instrument. Do not touch switches or electrical outlets with wet hands. Switch
the instrument off before disconnecting it from the mains supply.
9.3.2 Mechanical
The dispensing cassette is a free-moving mechanical device controlled by the instrument and not
directly by the operator. It is designed for “hands-off” operation and should be used as such.
Never reach into the work space, while the instrument is performing a dispensing cycle. If it is
necessary to stop the operation of the equipment, press the stop key on the control panel.
9.3.3 Environmental
Infectious samples and corrosive fluids are commonly used with this equipment. The “hands-off”
nature of the system allows the user to dispense into the reaction wells without getting into direct
contact with these fluids. However, the wells that have been in contact with potentially hazardous
fluids must be handled before and after the dispensing process, and this should be done with
utmost care. Hand and eye protections should always be worn as well as corrosive resistant
laboratory coats.
Observe normal laboratory procedures for handling potentially hazardous samples.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
72
Chapter 9: Troubleshooting
9.3.4 Defects and abnormal stresses
Whenever it is likely that the protection against safety hazards has been impaired, the instrument
should be made inoperative and be secured against any unintended operation.
The protection is likely to be impaired if, for example, the instrument:
1.
2.
3.
4.
shows visible damage;
fails to perform the intended functions;
has been subjected to prolonged storage under unfavorable conditions, or
has been subjected to severe transport stresses.
9.4 Service request protocol
If the Multidrop DW requires service, contact your local Thermo Fisher Scientific representative or
the Thermo Fisher Scientific technical service department. Do not under any circumstances send
the instrument for service without any prior contact. It is imperative to indicate the fault and nature
of the required service. This will ensure a faster return of the instrument to the customer.
Your local Thermo Fisher Scientific representative or distributor will take care of sending a
complaint form (that is, the Warranty Claim Technical Sheet) to the Thermo Fisher Scientific
technical service department. The Warranty Claim Technical Sheet contains a more detailed
description of the fault, symptom or condition. Give all the necessary information to the
distributor, who will fill out and forward the Warranty Claim Technical Sheet to the Thermo Fisher
Scientific technical service department.
Check Section 7.10 How to pack for service. You will find instructions on how to proceed before
shipping the instrument for service to Thermo Fisher Scientific Oy.
Check that any necessary decontamination procedure has been carried out before packing. See
Sections 7.8 and 9.5 on decontamination. Ensure that the Certificate of Decontamination as well as
the return authorization number (RGA) are sent with the instrument.
The Thermo Fisher Scientific technical service department will keep you up to date with the
progress of service and provide you with any further details you might need, for example, on
maintenance, serviceability, troubleshooting and replacement.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
73
Chapter 9: Troubleshooting
9.5 Certificate of Decontamination
The decontamination procedure is required prior to shipping the instrument to Thermo Fisher
Scientific Oy, for example, for repair. If, for any reason, the instrument is shipped back to Thermo
Fisher Scientific Oy, it must be accompanied by a dated and signed Certificate of
Decontamination, which must be attached to the outside of the package containing the
instrument. See Section 7.8 Decontamination procedure.
Failure to confirm decontamination will incur additional labor charges or at worst the items will be
returned for proper cleaning.
Before returning any instrument(s) or item(s), ensure that they are fully decontaminated. Confirm
A or B status:
Name:
Address:
Tel./Fax:
Name:
Serial no.:
A)
I confirm that the returned items have not been contaminated by body fluids, toxic, carcinogenic or
radioactive materials or any other hazardous materials.
B)
I confirm that the returned items have been decontaminated and can be handled without exposing the
personnel to health hazards.
Materials used in the unit:
Chemicals +
Biological •
Radioactive *)
Specific information
about contaminants:
Decontamination
procedure1:
Date and place:
Signature:
Name (block capitals):
*) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive
materials.
This unit is certified by the undersigned to be free of radioactive contamination.
Date and place:
Signature:
Name (block capitals):
PHOTOCOPIABLE
1
Please include decontaminating solution used.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
74
C h ap te r 10: W ar r an ty C e r ti fi c ate
10 Warranty Certificate
Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed
against defective parts and materials, including defects caused by poor workmanship, for a period
of one year from the date of delivery.
Thermo Fisher Scientific will repair or replace defective parts or materials during the term of
warranty at no extra charge for materials and labor provided that the products were used and
maintained in accordance with Thermo Fisher Scientific’s instructions. The warranty is invalid if
products have been misused or abused.
For the warranty to be effective, the product must have been purchased either directly from
Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor. The guarantee
is not transferable to a third party without prior written approval from Thermo Fisher Scientific.
This guarantee is subject to the following exclusions:
•
Any defects caused by normal wear and tear.
•
Defects caused by fire, lightning, flood, earthquake, explosion, sabotage, war, riot, or any
other occurrence of the type listed above.
•
Refurbished products that are subject to different warranty conditions.
THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES, INCLUDING
BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A
PARTICULAR PURPOSE. The seller is not liable for any loss or damage arising out of or in
connection with the use of the product or other indirect damages.
These warranty terms and conditions can be obtained from your local Thermo Fisher Scientific
dealer.
10.1 Warranty limitations
Consumables are not included in the warranty.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
75
Chapter 11: Ordering Information
11 Ordering Information
11.1 Instruments
Code
Instrument
5840170
5840177
Multidrop DW, 220 – 240 V 50/60 Hz
Multidrop DW, 100 – 120 V 50/60 Hz
11.2 Dispensing cassettes
Code
Dispensing cassette
24072670
24072671
24072672
24072677
Standard tube dispensing cassette (with 40 cm tubing set)
Standard tube dispensing cassette 5-pack
Standard tube dispensing cassette 10-pack
Long standard tube dispensing cassette (length of tubing 50 – 200 cm in 10 cm
increments, please specify the length when ordering)
Dispensing cassette Machined covers (with 40 cm tubing set) (Fig. 11.1)
Special dispensing cassette Multidrop, Machined covers (length of tubing 50 – 200
cm in 10 cm increments, please specify the length when ordering)
24070300
24070307
Fig. 11.1 Dispensing cassette, machined covers
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
76
Chapter 11: Ordering Information
11.3 Spare parts for dispensing cassettes
Code
Item
24070290
24070297
Tubing set for dispensing cassette (with 40 cm tubing set)
Special tubing set for dispensing cassette, special tubing set Multidrop (length of
tubing 50 – 200 cm in 10 cm increments, please specify the length when ordering)
Tube tip manifold
Tubing weight, eight tubes
Tubing weight, one tube
Tube holder assembly
1046200
1190780
1002430
2805980
11.4 Consumables
Code
Item
15280030
Priming vessel, high version
11.5 List of accessories
Code
Item
2406910
1507060
1610650
2305290
24072000
1012080
21040620
10260260
Volumetric calibration device
Multidrop DW User Manual
Dust cover
Serial cable RS-232C
1.1 ml Thermo Scientific test rack
Pump head adjustment plate (Fig. 6.17)
Plate adapter for normal plate (Fig. 6.9)
Plate adapter for 1.1 ml Thermo Scientific tubes (Fig. 6.9)
11.6 List of plates (consumables)
Code
3355
9502227
7571
9502887
7605
9502867
6310
95040450
9520770
Item
96-WELL MICROPLATES
Immulon 1B Plate, Flat bottom
96 Well Plate UB, Flat bottom
Microlite 1 + Plate, Flat bottom
White 96 Well Plate UB, Flat bottom
Microfluor 1 Black Plate, Flat bottom
Black 96 Well Plate UB, Flat bottom
Immulon 1 B 1x12 Strip Plate, Flat bottom
Microtiter 96 Deep Well Plate
Tube rack for 96 tubes
Qty
50 pcs/box
50 pcs/box
50 pcs/box
50 pcs/box
50 pcs/box
50 pcs/box
100 pcs/box
50 pcs/box
10 pcs/box
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
77
Chapter 11: Ordering Information
11.7 List of spare parts
Code
Item
Qty
1210810
1210850
Fuse 300 mA
Fuse 600 mA
10 pcs/box
10 pcs/box
11.8 List of recommended spare parts
Code
Item
1190760
1210810
1210850
Tension limiting wire
Fuse 0.6 A slow 5 x 20 (115 V)
Fuse 0.3 A slow 5 x 20 (230 V)
1 − 2 unit(s) / year
10 units / year
4
1
1
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
6
1
1
78
Chapter 12: Glossary and Abbreviations
12 Glossary and Abbreviations
Dispense
To distribute (pipette) liquid into the wells of the deep-well plate, tubes in a
96-channel rack, 96-well plate or the preselected strips.
Dispensing
cassette
The dispensing cassette contains eight individual tubes. The dispensing
cassette is detachable. Each reagent can have a separate dispensing cassette.
Alternatively, the same dispensing cassette can be used with several reagents
using the backflush feature. Refer to Section 11.2 Dispensing cassettes.
The dispensing cassettes contain either machined or extruded covers. The
tubing length also varies from 40 cm to a selectable length of 50 to 2000 cm.
All dispensing cassettes can be autoclaved up to 50 times.
To avoid damaging the tubes, follow the dispensing cassette maintenance,
washing, autoclaving and storage instructions carefully. Refer to Sections
6.8.4 After use, 7.2 How to wash the dispensing cassette and 7.3 How to
autoclave the dispensing cassette.
EIA
Enzyme immunoassay.
Empty
The operation of discharging the contents of the tubing of fluid.
EN
European Norm.
EU
European Union.
Home position
The plate carrier is positioned to the furthest right of the transfer rails (Fig. 4.1).
IEC
International Electrotechnical Commission.
Plate adapter
The Multidrop DW requires a plate adapter for the plate carrier to function when
you use a) 96-well plates or b) tubes in a 96-channel rack. Two different plate
adapters are included (Fig. 6.9).
If needed, insert the plate adapter correctly. Refer to Section 6.4 How to place
different types of plates.
PP
Polypropylene.
Prime
The operation of filling a pump intake with fluid to expel the air.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
79
Chapter 12: Glossary and Abbreviations
12.1 Keywords for web pages
automated dispensers
automated fluid dispensing
automated liquid handling
deep-well plate
deep-well plate dispenser
deep wells
dispensing
drug discovery
EIA
ELISA
enzyme immunoassay
immunoassay
liquid handling
microplate
microplate dispenser
Microtiter plate
microwell plate
multidispense
multidrop
Multidrop
Multidrop DW
multidrops
plate
robotic platform
sandwich assay
separation technique(s)
solid phase assay(s)
Thermo Fisher Scientific
Thermo Scientific
tubes in a 96-channel rack
12.2 Literature
Ennis, C. and Tanner, A. (Fall 1999): Analysis of CHO cell requirements for assay miniaturization in
high-throughput screening. The HTS Forum 8, 5 pages.
Hudson Control Group (Jan 2001): Integrating the Thermo Labsystems Multidrop with the
PlateCrane™. IB104A, 3 pages.
Long, H., Aldredge, T., Hebert, A., Perrin, S., Smith, M., August, P., Newcomb, R. and Call, K.
(October 2000): Automation technology applications in a genomics center. Presented at the
International Symposium on Laboratory Automation and Robotics, October 1999, Boston, MA.
American Laboratory, 30 – 35.
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
80
C
13 Index
1
1.1 ml tubes in a 96-channel rack ...................................................................................................29, 64
9
96-well plates.............................................8, 9, 14, 24, 25, 26, 27, 30, 36, 39, 44, 47, 61, 64, 68, 71, 79
A
Abbreviations ........................................................................................................................................79
About the User Manual ...........................................................................................................................7
Accessories................................................................................................................................15, 16, 77
Addresses ..............................................................................................................................................91
Adjustment plate .................................................................................................................32, 33, 34, 35
Advantages of using the Multidrop DW.................................................................................................9
Application...............................................................................................................................6, 9, 80, 90
B
Blanks.....................................................................................................................................................62
Bottle ........................................................................................................................................................9
Brief User’s Guide..................................................................................................................................89
C
Cable ..................................................................................................................15, 17, 18, 61, 65, 72, 77
Calibration..................................12, 22, 31, 37, 44, 45, 46, 47, 48, 50, 51, 55, 56, 57, 59, 60, 62, 77, 88
screw cover..............................................................................................12, 45, 46, 47, 48, 57, 59, 60
Channels ........................................................................................................................44, 45, 46, 47, 48
Check .............................................................................15, 16, 18, 20, 21, 22, 31, 32, 36, 43, 70, 73, 89
Clean ......................................................................................................16, 38, 39, 40, 41, 50, 62, 70, 74
Col ................................................................................................................14, 18, 30, 31, 36, 64, 68, 89
Columns.................................................................................................10, 14, 18, 30, 31, 36, 64, 68, 89
Consumables .............................................................................................................................31, 75, 77
Control panel ...................................................................................10, 18, 20, 30, 31, 40, 64, 72, 89, 90
indicators............................................................................................................................................30
keys.....................................................................................................................................................31
Cover retaining screws ...........................................................................................11, 49, 51, 53, 57, 58
D
Dead volume .........................................................................................................................................71
Decontamination ...........................................................................................................61, 62, 63, 73, 74
Certificate of .....................................................................................................................62, 63, 73, 74
Decontamination procedure.........................................................................................39, 61, 62, 73, 74
Deep-well plate........7, 8, 9, 11, 14, 20, 24, 30, 31, 32, 33, 34, 35, 36, 39, 44, 61, 64, 70, 71, 79, 80, 89
Dimensions ............................................................................................................................................64
Directives ...............................................................................................................................................66
Disclaimer ................................................................................................................................................5
Dispenser ...............................................................................................7, 8, 9, 14, 15, 18, 30, 39, 67, 80
Dispensing ..7, 8, 9, 11, 12, 14, 16, 18, 20, 21, 22, 24, 27, 28, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,
44, 46, 47, 49, 51, 52, 53, 54, 56, 57, 58, 59, 60, 61, 62, 63, 64, 68, 70, 71, 72, 76, 77, 79, 80, 88, 89
accuracy .............................................................................................................................................64
cassette ...7, 8, 9, 11, 12, 14, 16, 18, 20, 21, 22, 24, 27, 28, 31, 32, 33, 34, 38, 39, 40, 41, 44, 46, 47,
49, 51, 52, 53, 54, 56, 57, 58, 59, 60, 61, 62, 63, 64, 70, 71, 72, 76, 77, 79, 88, 89
precision.............................................................................................................................................64
volume..........................................................................................................................................64, 68
Disposal of instrument ..........................................................................................................................63
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
81
Chapter 13: Index
Disposal of materials.............................................................................................................................61
E
Empty.................................................................18, 20, 31, 36, 38, 39, 46, 47, 61, 64, 67, 68, 71, 79, 89
Environmental requirements ................................................................................................................16
Error ...................................................................................................................18, 30, 31, 39, 62, 69, 70
code....................................................................................................................................................69
message .............................................................................................................................................62
Extruded covers ....................................................................................................................8, 12, 76, 79
F
FAQ ........................................................................................................................................................71
Feedback Form......................................................................................................................................90
Frequently asked questions..................................................................................................................71
Fuse holder cap .........................................................................................................................13, 42, 43
Fuses ..............................................................................................................................13, 42, 43, 70, 78
G
Glossary .................................................................................................................................................79
Gravimetric calibration....................................................................................................................44, 46
Guidelines for use .................................................................................................................................39
H
Hazard ................................................................................................................................................6, 72
Home position .............................................................................................14, 18, 23, 31, 36, 39, 68, 79
How to autoclave the dispensing cassette ..............................................................................14, 41, 79
How to change the tip band .................................................................................................................49
How to check and adjust the pump rotor height for 50 mm high plates ...........................................32
How to disinfect the instrument .........................................................................................39, 61, 73, 74
How to dispense automatically ............................................................................................................36
How to dispense manually into the specified column(s) ....................................................................36
How to dispense multiple volumes......................................................................................................37
How to fit the new tubing set ...............................................................................................................50
How to install the Multidrop DW ..................................................................................15, 18, 19, 20, 65
How to maintain the instrument ...........................................................................................................40
How to pack for service ............................................................................................................61, 63, 73
How to place different types of plates ...............................................................................24, 36, 47, 79
How to prime.............................................................................................................................31, 36, 47
How to recalibrate the dispensing cassette...................................................................................44, 60
How to replace the fuses ......................................................................................................................42
How to shutdown ..........................................................................................................14, 38, 39, 41, 79
How to switch on...................................................................................................................................20
How to troubleshoot the instrument ....................................................................................................70
How to use the control panel .........................................................................................................18, 30
How to wash the dispensing cassette......................................................................................14, 41, 79
I
Indicators ...............................................................................................................................................30
Installation......................................................................................................7, 15, 16, 44, 46, 51, 65, 70
check list.............................................................................................................................................15
Instrument layout ..................................................................................................................................10
Intended use ............................................................................................................................................8
K
Keys..............................................................................................................14, 30, 31, 37, 39, 45, 64, 70
Keywords for web pages ......................................................................................................................80
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
82
C
L
LED .................................................................................................................................18, 20, 30, 39, 70
Literature................................................................................................................................................80
Live parts ...............................................................................................................................................65
Lower part....................................................................................................10, 12, 21, 22, 49, 51, 52, 53
M
Machined covers ...................................................................................................................................76
Mains........................................................................11, 13, 15, 17, 18, 20, 39, 42, 43, 61, 64, 65, 70, 72
switch .........................................................................................................................11, 17, 18, 20, 39
Maintain ...............................................................................................................................20, 40, 63, 89
Maintenance ..........................................................................7, 14, 20, 39, 40, 62, 63, 65, 72, 73, 79, 89
Microplate dispenser...............................................................................................7, 8, 9, 14, 39, 67, 80
Microstrip.............................................................................................................................39, 47, 48, 61
Microswitch ...........................................................................................................................................23
Mode ....................................................................................................................................14, 31, 37, 45
Multidrop DW ...7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 23, 24, 25, 30, 31, 36, 37, 39, 40, 44, 45, 46, 47, 65,
66, 67, 71, 73, 76, 77, 79, 80, 89, 90
O
Operating precautions and limitations.................................................................................................19
Operational check......................................................................................................................15, 18, 20
list .......................................................................................................................................................20
Ordering information ..............................................................................................................................7
P
Packing.......................................................................................................................................15, 63, 73
instructions...................................................................................................................................15, 63
list .......................................................................................................................................................15
materials.............................................................................................................................................63
Plate 7, 8, 9, 10, 11, 14, 18, 20, 23, 24, 25, 26, 27, 28, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 44, 45,
46, 47, 48, 49, 54, 57, 59, 61, 62, 63, 64, 67, 68, 70, 71, 77, 79, 80, 88, 89
adapter .......................................................................20, 24, 25, 26, 27, 28, 36, 47, 62, 63, 77, 79, 89
carrier ...........................................10, 11, 14, 18, 23, 24, 25, 26, 27, 28, 31, 32, 36, 37, 39, 40, 70, 79
switch .................................................................................................................................................68
type.....................................................................................................................................................68
Precalibration...................................................................................................................................59, 60
Prime 10, 18, 20, 21, 23, 31, 32, 33, 36, 37, 38, 39, 41, 44, 45, 46, 47, 62, 64, 68, 69, 70, 71, 77, 79, 89
Prime/drop ...................................................................................................18, 31, 36, 37, 45, 47, 64, 89
Priming vessel ...........................10, 18, 20, 21, 23, 31, 32, 33, 36, 37, 44, 46, 47, 68, 69, 70, 71, 77, 89
Protocol......................................................................................................................................62, 73, 88
Pump body ................................................................................................................................10, 21, 22
Pump head adjustment plate........................................................................................32, 33, 34, 35, 77
Pump rotor.....................10, 11, 18, 20, 21, 22, 23, 24, 27, 28, 31, 32, 33, 34, 35, 38, 39, 44, 46, 70, 89
Purge......................................................................................................................................................79
R
Remote control ......................................................................................................................................67
Rest position ..........................................................................................10, 20, 24, 38, 39, 41, 44, 47, 89
Robotic...................................................................................................................................................80
Robotics .................................................................................................................................................80
Rotor ..............................10, 11, 18, 20, 21, 22, 23, 24, 27, 28, 31, 32, 33, 34, 35, 38, 39, 44, 46, 70, 89
cover.....................................................................10, 11, 18, 20, 23, 24, 27, 28, 31, 32, 44, 46, 70, 89
needles ...................................................................................................................................10, 23, 70
shaft ..............................................................................................................................................21, 23
Routine.....................................................................................................................................7, 8, 20, 40
Run .............................................................................................................................................62, 88, 89
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
83
Chapter 13: Index
S
Samples .....................................................................................................................................16, 61, 72
Service ...............................................................................................................40, 41, 61, 62, 63, 70, 73
contracts.............................................................................................................................................63
pack for...................................................................................................................................61, 63, 73
request protocol ................................................................................................................................73
Shutdown ..............................................................................................................................................39
Sight strips.................................................................................................................................12, 59, 60
Sight window...................................................................................................................................54, 55
Silicone tubing...........................................................................................................................31, 51, 71
Slots ...............................................................................................................................23, 31, 44, 69, 70
Software.............................................................................................................................................7, 68
Spare parts ......................................................................................................................................77, 78
recommended....................................................................................................................................78
Specifications ..............................................................................................................................7, 64, 65
general................................................................................................................................................64
safety ..................................................................................................................................................65
Startup .......................................................................................................................................17, 20, 68
Step ........................................................................................................14, 18, 31, 36, 37, 39, 45, 50, 64
Switching on..........................................................................................................................................17
Symbols ...................................................................................................................................................6
T
Table of Figures.....................................................................................................................................86
Tension limiting wires .................................................................................12, 21, 22, 23, 51, 52, 56, 78
Thumbwheels ........................................................................................................................................37
Time .......................................................................................................................................................62
Tip ..........................................................................................................34, 44, 49, 50, 51, 61, 70, 71, 77
band............................................................................................................................49, 50, 51, 61, 71
manifold .................................................................................................................................49, 51, 77
protection pins ...................................................................................................................................34
Transfer rails ....................................................................................................................................10, 79
Transport .......................................................................................................................15, 16, 62, 64, 73
damage.........................................................................................................................................15, 16
package ..................................................................................................................................15, 16, 62
Troubleshooting ..........................................................................................................................7, 70, 73
guide...................................................................................................................................................70
Tube racks ...........................................................................................................................24, 25, 64, 77
Tubes .....................................................24, 25, 31, 36, 44, 45, 46, 47, 49, 51, 52, 55, 59, 60, 64, 70, 77
in a 96-channel rack.......................................................8, 9, 14, 25, 27, 28, 29, 30, 36, 39, 61, 79, 80
Tubing ........................8, 12, 18, 24, 31, 36, 38, 39, 41, 49, 50, 51, 52, 54, 55, 56, 60, 62, 71, 76, 77, 79
identification plate .............................................................................................................................54
set .......................................................................................8, 24, 33, 41, 49, 50, 51, 60, 62, 71, 76, 77
weight.............................................................................................................12, 31, 36, 50, 51, 60, 77
U
Unpack ...................................................................................................................................................15
Upper part..............................................10, 12, 21, 22, 38, 39, 44, 45, 46, 47, 51, 53, 54, 56, 57, 58, 59
User manual...........................................................................................................................7, 15, 62, 77
V
View .....................................................................................................................................10, 11, 13, 14
front ..............................................................................................................................................10, 11
internal................................................................................................................................................14
rear .....................................................................................................................................................13
Voltage .................................................................................................................................16, 19, 66, 72
Volumetric calibration ...................................................................................................31, 37, 44, 45, 77
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
84
C
W
Warnings..........................................................................................................................................16, 17
Warranty ......................................................................................................................................7, 15, 75
Weight....................................................................................................12, 16, 31, 36, 50, 51, 60, 64, 77
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
85
Chapter 14: Table of Figures
14 Table of Figures
Fig. 3.1 Multidrop DW deep-well plate dispenser ..................................................................................8
Fig. 4.1 Multidrop DW front view (A).....................................................................................................10
Fig. 4.2 Multidrop DW front view (B).....................................................................................................11
Fig. 4.3 Multidrop DW front view (C).....................................................................................................11
Fig. 4.4 Close-up of the Multidrop DW dispensing cassette, extruded covers .....................................12
Fig. 4.5 Multidrop DW rear view ...........................................................................................................13
Fig. 4.6 Multidrop DW deep-well plate dispenser ................................................................................14
Fig. 5.1 Connecting the mains supply cable..........................................................................................17
Fig. 6.1 Inserting the dispensing cassette..............................................................................................21
Fig. 6.2 Inserting the lower part of the dispensing cassette into its slots ..............................................21
Fig. 6.3 Inserting the upper part of the dispensing cassette..................................................................22
Fig. 6.4 Lower and upper parts of the dispensing cassette inserted evenly into their slots..................22
Fig. 6.5 Inserting the priming vessel into place .....................................................................................23
Fig. 6.6 Pulling the rotor cover over the rotor........................................................................................23
Fig. 6.7 Deep-well plate placed onto the plate carrier ..........................................................................24
Fig. 6.8 Micronic tube rack placed onto the plate carrier......................................................................25
Fig. 6.9 Two different plate adapters .....................................................................................................26
Fig. 6.10 Plate adapter for the 96-well plate inserted into the plate carrier ..........................................26
Fig. 6.11 96-well plate placed onto the plate adapter ...........................................................................27
Fig. 6.12 Plate adapter for tubes in a 96-channel rack inserted into the plate carrier ...........................28
Fig. 6.13 Tubes in the 96-channel rack placed onto the plate adapter..................................................28
Fig. 6.14 1.1 ml tubes in the 96-channel rack........................................................................................29
Fig. 6.15 Multidrop DW control panel ...................................................................................................30
Fig. 6.16 Location of the adjustment screws that may have to be adjusted..........................................32
Fig. 6.17 Placing the adjustment plate onto the deep-well plate ..........................................................32
Fig. 6.18 Moving the deep-well plate and adjustment plate under the dispensing cassette ...............33
Fig. 6.19 Loosening the adjustment screws ..........................................................................................33
Fig. 6.20 Moving the deep-well plate under the dispensing cassette ..................................................34
Fig. 6.21 Tip protection pins resting on the adjustment plate................................................................34
Fig. 6.22 Fastening the adjustment screws ...........................................................................................35
Fig. 6.23 Dispensing into a deep-well plate once the pump rotor height has been adjusted ..............35
Fig. 6.24 Rest position of the dispensing cassette.................................................................................38
Fig. 7.1 Removing the fuse holder cap (A) ............................................................................................42
Fig. 7.2 Removing the fuse holder cap (B) ............................................................................................42
Fig. 7.3 Fuse holder cap with fuses .......................................................................................................43
Fig. 7.4 Inserting the fuse holder cap ....................................................................................................43
Fig. 7.5 Placing the tube onto the tip of channel A ...............................................................................44
Fig. 7.6 Adjusting the liquid level first to the lower mark and then to the upper mark .........................45
Fig. 7.7 Screwing the calibration screws according to the calibration results ......................................46
Fig. 7.8 8 x 12 well microstrips adjusted onto the plate adapter ...........................................................47
Fig. 7.9 Removing the filled microstrip to be weighed .........................................................................48
Fig. 7.10 Screwing the calibration screw according to the calibration results......................................48
Fig. 7.11 Removing the cover plate of the lower part of the dispensing cassette ................................49
Fig. 7.12 Removing the old tip band......................................................................................................49
Fig. 7.13 Tip band removed ...................................................................................................................50
Fig. 7.14 New tip band...........................................................................................................................50
Fig. 7.15 New tubing set with the tip band attached.............................................................................51
Fig. 7.16 Inserting the tip manifold into the lower part of the dispensing cassette ..............................51
Fig. 7.17 Pressing the tube fasteners into place ....................................................................................52
Fig. 7.18 New tubing inserted into the lower part of the dispensing cassette......................................52
Fig. 7.19 Tension limiting wires attached ..............................................................................................52
Fig. 7.20 Replacing the lower part cover of the dispensing cassette....................................................53
Fig. 7.21 Parts belonging to the upper part of the dispensing cassette ................................................53
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
86
Chapter 14: Table of Figures
Fig. 7.22 Placing the sight window........................................................................................................54
Fig. 7.23 Pressing the tubing identification plate into place..................................................................54
Fig. 7.24 Pressing the tube fasteners into place ....................................................................................55
Fig. 7.25 Fastening the calibration screws.............................................................................................55
Fig. 7.26 Calibration screws fastened ....................................................................................................56
Fig. 7.27 Replacing the cover of the upper part of the dispensing cassette .........................................56
Fig. 7.28 Ensuring the tubing does not get trapped ..............................................................................56
Fig. 7.29 Fastening the cover retaining screws .....................................................................................57
Fig. 7.30 Sliding the plate onto the calibration screws ..........................................................................57
Fig. 7.31 Lower and upper part of the dispensing cassette fitted .........................................................58
Fig. 7.32 Dispensing cassette before precalibration..............................................................................59
Fig. 7.33 Precalibrating the dispensing cassette ...................................................................................59
Fig. 7.34 Dispensing cassette precalibrated ..........................................................................................60
Fig. 7.35 Inserting the tubes into the tubing weight..............................................................................60
Fig. 11.1 Dispensing cassette, machined covers ..................................................................................76
Fig. Appendix B.1 Multidrop DW keypad ..............................................................................................89
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
87
A ppendi x A : Sy stem L og
Appendix A. System Log
Instrument name/number:
User
Date
Date of calibration /
dispensing cassette change /
tubing set change
Liquid dispensed
# Plates run
Comments
PHOTOCOPIABLE
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
88
A p p e n d i x B : T h e r m o S c i e n t i fi c M u l t i d r o p D W B r i e f U se r ’ s G u i d e
Appendix B. Thermo Scientific Multidrop DW Brief User’s
Guide
•
Insert the dispensing cassette to be used (p. 21).
•
Check that the rotor cover is pulled over the rotor (p. 20).
•
Check that the priming vessel is installed (p. 21).
•
Use the correct plate adapter if needed (p. 24).
•
Switch the Multidrop DW on (p. 20).
•
Load the deep-well plate to be dispensed onto the instrument (p. 24).
•
Select the desired volume and column(s) from the control panel (p. 30).
•
To prime the tubes, press the prime/drop key until the reagent flows from
the tips into the priming vessel (p. 31).
•
Press the start key to start dispensing (p. 31).
•
After the end of the run, remove the deep-well plate (p. 38).
•
Press the empty key to remove the reagent from the dispensing cassette
(p. 38).
•
Put the dispensing cassette into the rest position (p. 38).
•
Maintain your Multidrop DW instrument and dispensing cassette regularly
(p. 40).
•
Switch off the instrument (p. 39).
Fig. Appendix B.1 Multidrop DW keypad
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
89
A p p e n d i x C : T h e r m o S c i e n t i fi c M u l t i d r o p D W F e e d b ac k F o r m
P l e ase se n d t o T h e r m o F i sh e r S c i e n t i fi c O y : F ax + 358-9-32910415
Appendix C. Thermo Scientific Multidrop DW Feedback Form
Cat. no.
Serial no.
PURCHASED BY
PURCHASED FROM
Company/Institute
Distributor
Department
Address
Address
Tel.
Tel.
Fax
Date of delivery
Internet home page
Date of purchase
Your research area
Dr. … Mr. … Mrs. … Ms. … Job title/Position
Surname (block capitals)
First name (block capitals)
Internet e-mail address
Excellent
Reagent kit/Instructions
…
Above
expectations
…
As
expected
…
Below
expectations
…
Instrument/User manual
…
…
…
…
Operational reliability
…
…
…
…
Design
…
…
…
…
Ease of use
…
…
…
…
Operational costs
…
…
…
…
Customer support
…
…
…
…
Comments
Further instrument/system developments desired:
Further applications desired:
Where did you first learn about the product?
Would you like to receive information about other Thermo Scientific products?
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
90
A ppendi x D: A ddr esses
Appendix D. Addresses
For the latest information on products and services, visit our websites at:
http://www.thermo.com
http://www.thermo.com/multidrop
Manufactured by:
Distributed by:
Thermo Fisher Scientific Oy
P.O. Box 100, FI-01621 Vantaa, Finland
Tel. +358-9-329 100, Fax +358-9-3291 0415
www.thermo.com
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
91
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
92
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
93
Thermo Scientific Multidrop® DW User Manual Rev. 3.2, Cat. no. 1507060
94
Thermo Fisher Scientific Oy
Microplate Instrumentation
Ratastie 2, P.O. Box 100
FI-01621 Vantaa
Finland
www.thermo.com
1507060