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Page: 25 of 35 FSPS User Manual Chapter 3.1 (Control Tab 0, Continuous or Triggered acquisition) Allows you to choose between continuous (Chapter 2) and triggered (this chapter) acquisition depending on your needs and experimental design. Chapter 3.2 (Control Tab 1, hardware info & settings) PSTH internal tab (hardware-based) Commonly, electrophysiological recording systems contain an option to perform spike isolation using hardware voltage discriminator. This makes it possible to roughly characterize neuronal activity and its specificity instantly and during recording process. This function is also offered by this software, in parallel with the Fuzzy Spike Sorting procedure. Please note, that to acquire the signal from your window discriminator hardware (in our case it is channel 2), you need to reserve a separate I/O channel at your DAQ . “A” can be used to switch auto scale for PSTH graph ON (green) or OFF (dark). UNIFE, Section of Human Physiology, 2012