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Appendix D PDQuest Version 7.0 and 7.1 The following procedure applies to new installations of PDQuest version 7.0 and 7.1 for the ProteomeWorks Plus Spot Cutter. This is for installations on a system where no other Roper Scientific software exists. Important Note: Install the camera driver software BEFORE physically installing the PCI card hardware in the computer. Installation Procedure 1. Insert The Discovery Series / PDQuest install CD in the CD-ROM. The disk will start up and show the "Discovery Series" Window. 2. Select "Explore CD". 3. Select PDQUEST folder. 4. Select CoolSNAP folder (Readme file has detailed instructions for installing PVCam Setup) 5. Select PVCamSetup. Follow on screen instructions to set up the camera software. Select standard installation Use the default destination folder location C\: Program Files\Roper Scientific\PVCam 6. When software installation is complete, select "No. I will restart my computer later," remove CD, click finish, and shut down computer. 7. Install the PCI Card in an open PCI slot on the computer mother-board and connect the camera cable. Note: Complete Spotcutter, camera and cable setup before proceeding to Step 8. 8. Reboot computer and turn camera on. 9. Run "RSConfig" program (Start\Programs\RoperScientific\RSConfig.) to confirm that camera name is entered. Do not rename the camera in the RSConfig program, leave the default name "Camera 1". 10. Run PVCam test to confirm that camera is functional. Make sure that the camera is on and connected to the PCI Card. Start\Program|Roper Scientific\PVCam test. 11. Click on collect image to acquire an image and verify camera functionality. 12. When completed, install PDQuest software as described in the PDQuest manual. Troubleshooting Camera Driver and PCI Card Installation The camera driver for the ProteomeWorks Plus spot cutter camera exists on the install disk for the Discovery Series software. Beginning with PDQuest version 7.0, the camera driver software has changed to a new version (version 2.5.8). The installation procedure for new installations has changed significantly from the earlier version of the camera driver (which existed on the PDQuest 6.2.1 version). The new procedure has been included with the spot cutter manual 4006196 beginning with Revision E. 59