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A: 2%
G: 1%
T: 97%
G: 1%
T: 99%
400
300
200
100
0
E
S
A
T
C
G
T
5
G
C
A
A
G
10
C
A
T
G
C
15
T
G
Figure 8. Pyrogram trace obtained after analysis of a sample with a normal genotype
in codons 858–861 with the “Sequence to Analyze” CKGGCCAAACDGCTGGGT.
---------------: 1%
GGAATTAAGAGAAGC: 99%
200
150
100
50
0
E
S
C
T
A
T
C
5
A
C
T
G T C
10
A
G C
T C
15
G A
T
C G T
20
C
A
T C
25
G T
C
A C
30
G C
Figure 9. Pyrogram trace obtained after analysis of a sample with a normal genotype
in exon 19.
---------------: 33%
GGAATTAAGAGAAGC: 67%
250
200
150
100
50
0
E
S
C
T
A
T
C
5
A
C
T
G T C
10
A
G C
T C
15
G A
T
C G T
20
C
A
T C
25
G T
C
A C
30
G C
Figure 10. Pyrogram trace obtained after analysis of samples with a
GGAATTAAGAGAAGC deletion in exon 19.
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EGFR Pyro Handbook 09/2010