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Applied Biosystems
Figure 6-3.
Protected exocyclic base amines. Adenosine (A) and cytosine (C) are protected by a
benzoyl group (Bz), and guanosine (G) by an isobutyryl group (Ib). Thyrnidine (T)
does not need a protecting group.
DNA Synthesis Chemistry Cycle
Each cycle of base addition consists of four steps:
1. Detritylation
2. Coupling
3. Capping
4. Oxidation.
These reaction steps are repeated in the above order until all bases are added. Following synthesis,
the DNA chain must be manually cleaved and deprotected from the solid support. Each step will be
discussed in detail.
Section 6: Chemistry for Automated DNA Synthesis
6-7