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CONVERSION OF HYDROGEN-PHOSPHONATE
TO PHOSPHATE ANALOGS
Figure 6-12.
5' Attachments
In the last several years many applications have been identified and developed for the covalent attachment of small molecules to oligonucleotides. These molecules include fluorescent dyes30,
biotin31, proteins32, and other species that allow the identification of oligonucleotides in biological
systems. In addition, oligonucleotides can be derivatized to allow attachment to solid supports.33 In
this way, for example, an affinity matrix can be constructed to purify a sequence complementary to
the support-bound sequence.
The attachment can be made at several sites on the oligonucleotide. However, this site must not impair hybridization or confer chemical instability. For these reasons and for synthesis ease and efficiency, the 5' terminus is usually the preferred location for derivatization of an oligonucleotide.
Section 6: Chemistry for Automated DNA Synthesis
6-27