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tmd017.0708.qxp 7/25/2008 4:12 PM Page 25 Processing Sample Data for Databasing or Paternity Samples Import sample files into a new project as described in the Applied Biosystems GeneMapper ® ID Software Human Identification Analysis Tutorial. 2. In the Sample Type column, use the drop-down menu to select “Ladder”, “Sample”, “Positive Control” or “Negative Control”. Every folder in the project must contain at least one ladder that is designated as such for proper genotyping. 3. In the Analysis Method column, select the analysis method created in the Creating a Databasing or Paternity Analysis Method section. 4. In the Panel column, select “PowerPlex_ES_ID3.2.X”, where “X” refers to the most recent version of the panel files. This is the panel set that was imported in Section 6.A. 5. In the Size Standard column, select the size standard that was created in Creating a Size Standard section. 6. If analyzing data from an ABI PRISM® 310 Genetic Analyzer, ensure that the appropriate matrix file is selected in the Matrix column. 7. Select “Analyze” (green arrow button) to start the data analysis. 5785TB 1. Figure 7. The Allele tab with settings for using a 20% peak filter. Select the bin set “Promega_Bins_ID3.2.x.txt”, where “x” refers to the most recent version of the bin set. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Revised 7/08 Part# TMD017 Page 25