Download Multilocus Variable Number Tandem Repeat - MIRU

Transcript
In contrast, the occurrence of 2 alleles is observed in a single locus rather suggests the
presence of a given allelic variant within a clonal isolate.
2.6.2.11 Viewing and analyzing the second half of the capillary lanes
If applicable, the second half of the capillary lanes can be analyzed according to the same
procedure as described above.
To select this half, click the Dye/Lane Window button, then select the appropriate dyes, by
clicking the dyes from the first lane and then Shift+clicking the dyes from the last lane. Note: when
using a 96-capillary system, include also the last sample from the first half, to control the
occurrence of potential fluorescence cross-talks.
2.6.2.12 Final sorting by file names
After analysis of all capillaries, all sample files should be sorted by file names, as indicated
below. This mode of final sorting choice is more reliable and convenient to compare result tables
from different analysis runs, among which sample plate mappings are not necessarily identical.
1. To select all samples, click the Dye/Lane Window button, then press Ctrl+A to
select all lanes.
2. From Genotyper main menu, select Views, then Dye/Lane Sorting, then select
Sample Name and Dye Color from the first and second pull-down menus,
respectively, both in ascending order, and then press OK. Note: this sorting can also
be performed after the creation of the final results table, by selecting Table from the
main menu, then Sort Table, and selecting Sample Info from the first pull-down
menu.
2.6.2.13 Creating final results tables and verifying results
Final results tables (see example below) are created using a macro called “final table” in the
Genotyper templates. These tables are used to check, and subsequently export the results.
Columns of interest in this final table include the following information:
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Sample information
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