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Understanding the ChromAlign Process
Aligning Over Different Abundance Levels
Aligning Over Different Abundance Levels
Biomarker discovery detects low abundance components in the presence of high abundance
components that remain essentially unchanged between the controls and samples. The
following example shows the alignment of low abundance components in the presence of high
abundance components. Figure 20 shows reconstructed ion chromatograms of a horse
myoglobin peptide added to the nine-protein mix at three different concentrations (500 fmol
[red], 250 fmol [green], and 100 fmol [blue]) and compared to the control sample (1000 fmol
[black]).
50000
0
Abundance
100000
150000
Figure 20. Original reconstructed ion chromatograms
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SIEVE User Guide
Thermo Fisher