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Table of Contents
SUBJECT
1 Introduction
2 Items of Particular Interest
3 Cell Electrofusion Tutorial
3.1 Applications
3.1.1 Hybridoma Production
3.1.2 Immunotherapy
3.1.3 Nuclear Transfer
3.2 Cell Fusion Methods
3.3 Electrofusion
3.3.1 Cell Alignment (Dielectrophoresis)
3.3.2 Waveforms for Electrofusion
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3.3.2.1 Cell Alignment and Compression
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3.3.2.2 AC Sinewave Waveforms
19-22
3.3.2.3 Electrofusion Pulse
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3.3.2.4 Post Fusion Pulse Sinewave
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3.3.2.5 Parameters Range of HybrimuneTM Waveform Generator
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3.3.2.6 Published Waveform Protocols
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4 Hybrimune™ System Components and Set-up
4.1 Introduction24
4.2 The Hybrimune™ Waveform Generator
4.3 The Large Volume Chambers
4.4 Cytofusion® Medium
4.5 Setting up the Hybrimune™ System
4.6 Waveform Generator Front Panel Display
4.6.1 System Status Group
4.6.2 Process Status Group
4.7 Waveform Generator Interface Software Installation
4.7.1 Set-up using a USB-to-Serial Converter
4.8 System Test
4.9 Elementary Verification Tests
4.10 The Waveform Generator Interface Software
4.10.1 The Tool Bar
4.10.2 Tools Area
4.10.3 Status Area
4.10.4 Last Protocol Log Window
4.10.5 Control Panel Area
4.10.6 Running a Protocol
4.10.7 File Management
4.11 Programming Waveform Protocols
4.11.1 Programming the Protocol
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Hybrimune™ Hybridoma Production System • User’s Manual
(toll free) 800.272.2775 • 508.893.8999 • www.btxonline.com
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