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Items of Particular Interest
The Hybrimune™ Commercial Electrofusion System is the most sophisticated system available for fusing dissimilar
cells. The system is available only under license directly from Cellectis. The following are important:
1. The System must remain in the possession of the licensee
2. The System has four components, the Hybrimune™ Waveform Generator, the user interface software,
the fusion chambers and the Cytofusion® medium. These components were all designed to operate as a
system. Using other components may damage the system and will certainly provide degraded performance.
3. The System shall only be used as described in this User Manual.
4. Certain concepts and processes are covered by BTX patents and patents pending.
5. Other ionic medium shall not be used; it will generate significant heating and kill cells.
6. The chamber must be cleaned after each use to prevent ionic build up which will cause heating and
kill cells and reduce efficiency.
7. The cover must be used on the chamber while the waveforms are being applied.
8. Do not connect or disconnect the chamber from the waveform generator when the waveforms are being
applied to the chamber.
9. Do not put your fingers or any other object in the chamber while the waveforms are being applied to the chamber.
10. This system produces voltages as high as 1000 volts and may cause injury or death if used improperly.
11. The user must read the User Manual. If the user has any questions, contact BTX.
BTX would welcome any comments from the users to improve this manual.
Hybrimune™ Hybridoma Production System • User’s Manual
(toll free) 800.272.2775 • 508.893.8999 • www.btxonline.com
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