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Automated Protocol d Note what step the plate is queued for, and proceed with that step. For information about how to use Illumina LIMS, see the Illumina LIMS User Guide. Steps to Incubate MSA3 Plate OVERNIGHT INCUBATION Incubate MSA3 plate in the Illumina Hybridization Oven for at least 20 hours but no more than 24 hours at 37°C. 1 Record the start and stop times on the lab tracking form. NOTE To record information about your assay such as operator information, start and stop times, and barcodes, use the lab tracking form provided at www.illumina.com/documentation. This form can be filled out and saved online, or printed and filled in by hand. 2 122 Proceed to the next step. Part # 15045738 Rev. A