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38 VPrep User Manual 3.0 Mix The Mix task repeatedly aspirates and dispenses a liquid sample in a single plate or reservoir. The Mix task can be used to mix samples or to wash the pipette tips in a reservoir or a MicroWash tray (see Figure 23). Parameter Definition Shelf Specifies the shelf in which to mix. For information on configuring the shelves, see “Configure Shelves” on page 31. Pre-aspirate volume Specifies the volume of air to draw into the tip before the first aspiration stroke. Mixing volume Specifies the volume of fluid to be repeatedly aspirated and dispensed. Blowout volume Specifies the volume dispensed from the tips following the last mix step. The tips are still in the wells. Mixing cycles Specifies how many times to repeat the aspirate/ dispense cycle. Quadrant(s) Specifies the quadrant(s) in which to mix. Tips distance from well bottom Specifies the distance (in mm) from the bottom of the well at which the mix operation will be performed. Liquid class Specifies the liquid class used in the mix task. Aspirate velocity Specifies the speed at which the fluid is aspirated during the mix cycle. Aspirate acceleration Specifies the acceleration at which the fluid is aspirated. Dispense velocity Specifies the speed at which the fluid is dispensed during the mix cycle. Dispense acceleration Specifies the acceleration at which the fluid is dispensed. Dynamic tip retraction Specifies the distance in millimeters to lower the head for each microliter aspirated and raise for each microliter dispensed.